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Revista de biología marina y oceanografía

On-line version ISSN 0718-1957

Abstract

RODRIGUEZ-IBARRA, L. Estela et al. Effect of eggs desticking using protease proteolytic enzyme and pineapple juice in the larviculture of the puffer fish Sphoeroides annulatus. Rev. biol. mar. oceanogr. [online]. 2013, vol.48, n.2, pp.379-385. ISSN 0718-1957.  http://dx.doi.org/10.4067/S0718-19572013000200017.

Recent findings show that eliminating the gelatinous egg capsule surrounding the fertilized eggs of the puffer fish Sphoeroides annulatus improves embryonic development and hatching rate. In this work, the following 3 different desticking procedures were used to evaluate larval growth and survival: 5 ml L-1 proteolytic protease enzyme (EPP), 1% of pineapple juice (JP) and eggs with no sticky coating on the surface as a control (C). All treatments were carried out in triplicate and the experiment lasted for 33 days. The fertilization percentage obtained was 98.6 ± 1.8% in all treatments. The highest hatching percentage was achieved with EPP (93.03 ± 3.01%) and the lowest with JP (80.97 ± 3.97%). Both total length (LT) (14.4 ± 0.40 mm) and weight (P) (0.118 ± 0.004 g) were significantly higher in the JP treatment. The highest survival rate was obtained with EPP (19.34 ± 1.98%) and differences were found to be statistically significant. The desticking of eggs treated with JP gave a low hatching percentage due to the fruits pH reading of 4 and since fewer organisms were produced, higher growth rate was obtained. Thus, we conclude that removing the adhesive layer of the egg does not affect larval growth and survival.

Keywords : Egg adhesiveness; proteolytic enzyme; pineapple; larvae culture; Sphoeroides annulatus.

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