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Revista de biología marina y oceanografía

On-line version ISSN 0718-1957


ARRIAGADA, Daniel; LEPEZ, Irene; RUIZ, Maryori  and  CONTRERAS, Ingrid. Induction of spawning of the razor clam Ensis macha by injection of serotonin. Rev. biol. mar. oceanogr. [online]. 2013, vol.48, n.3, pp.653-660. ISSN 0718-1957.

The effect of serotonin spawning stimulating agent, was evaluated in Ensis macha sexually mature broodstock, conditioned in laboratory. Neurotransmitter 0.9 ml at a concentration of 10-5 M was injected into the foot of each specimen. 5-HT induced gamete release in 92% of injected broodstock, always producing the fastest response in males, removing sperm at 36 min (± 15.9) after injection. The release of mature oocytes began at 170 min (± 42.4) post-injection. Embryonic development to the veliger larva formation, straight hinge D had a survival of 88%, no malformations have been observed in larvae. In only one trial, fertilized oocytes were not viable. Finally, we can confirm that there is a pattern in the release of mature gametes E. macha broodstocks due to the action of serotonin 5-HT and could be a useful tool for aquaculture practices.

Keywords : Ensis macha; serotonin; spawning.

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