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Revista de biología marina y oceanografía

On-line version ISSN 0718-1957

Abstract

MOLINA-CARDENAS, Ceres A; SANCHEZ-SAAVEDRA, M. del Pilar  and  LICEA-NAVARRO, Alexei Fedorovish. Decreasing of bacterial content in Isochrysis galbana cultures by using some antibiotics. Rev. biol. mar. oceanogr. [online]. 2016, vol.51, n.1, pp.101-112. ISSN 0718-1957.  http://dx.doi.org/10.4067/S0718-19572016000100010.

The axenic microalgae cultures are a difficult task and they are hard to maintain. Microalgae cultures with reduced bacterial load can be an option to axenic microalgae cultures to produce compounds with biotechnological and pharmaceutical potential. Also they can be used for cryopreservation and in biochemical, physiology, ecology and genetic studies. The aim of this study was to develop a protocol to decrease the bacterial load in an Isochrysis galbana culture, through washes by centrifugation and the administration of various antibiotics (ampicillin, neomycin, kanamycin, chloramphenicol, sulphate G418, streptomycin, and carbencillin), at several doses and combinations. The concentrations of heterotrophic bacteria and I. galbana cell densities were monitored daily. Maximum non-lethal concentration and lethal concentration 50% (LC50) were calculated. Individually, antibiotics and washes by centrifugation failed to reduce bacterial load, but their combination removed bacteria from the cultures. Peak survival (84.6 ± 1.4%) and reduction of bacterial load in I. galbana cultures were effected with the combination of 5 washes by centrifugation and administration of a cocktail, comprising ampicillin, kanamycin, neomycin, and streptomycin at 48 h. Values of maximum non-lethal concentration varied from 75 to 106 µg mL-1 and LC50 between 194 and 332 µg mL-1, thus, our protocol is an effective and rapid method of producing I. galbana cultures with reduced bacterial load.

Keywords : Bacterial load; Isochrysis galbana; antibiotics; centrifugation.

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