Resumen

KARAMI, Leila et al. Oak wood inhabiting fungi and their effect on lignin studied by UV microspectrophotometry. Maderas, Cienc. tecnol. [online]. 2014, vol.16, n.2, pp.149-158.  Epub 17-Mar-2014. ISSN 0718-221X.

Decayed-wood samples were collected from a naturally-infected bridge made of Quercus robur. Fruiting bodies of the white-rot basidiomycetes Hymenochaete rubiginosa and Stereum hirsutum were sampled. The white-rot fungus Fuscoporia ferrea and the secondary saprobiont Mycena galericulata were identified from the rotten wood by means of rDNA-ITS sequencing. The topochemistry of lignin degradation within individual cell-wall layers was determined by cellular UV-microspectrophotometry (UMSP) at 278 nm wavelength. Increased delignification occurred in compound middle lamella regions (CML), secondary wall layers of fibres (S2), longitudinal and ray parenchyma cells as well in vessels. The highest lignin content at initial and medium decay was recorded in the CML, whereas in advanced decay secondary wall layers of the vessels exhibited the highest content of residual lignin. In all stages of degradation, the S₂ layers of fibres had the lowest lignin amount.

Palabras clave : Oak wood degradation; fungi; rDNA-ITS sequencing; lignin content; UV-microspectrophotometry.

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