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Ingeniare. Revista chilena de ingeniería

versão On-line ISSN 0718-3305


STREIT, Nívia M. et al. Pigment production by Aphanothece microscópica Nageli from dairy industrial waste. Ingeniare. Rev. chil. ing. [online]. 2017, vol.25, n.2, pp.350-358. ISSN 0718-3305.

The systems that use microalgae to obtain bioproducts are considered a promising area for industrial applications. The objective of this work was to evaluate the production of phycobiliproteins and chlorophyll-a by Aphanothece microscopica Nageli, when it is cultivated in autotrophic and heterotrophic systems. Thus, the cultures were grown in heterotrophic (absence of light) and autotrophic (2 klux of light intensity) system, in bioreactors at pH 7.6, 20 °C, 1 vvm aeration, using a dairy industry effluent, with C/N 20 and N/P 10, and BG11 as culture media. Initial inoculum 200 mg/L. The concentrations of chlorophyll-a andphycobiliproteins in logarithmic and stationary phase were monitored. The highest concentrations of pigment were observed in logarithmic phase: The highest concentrations of chlorophyll-a in autotrophic cultures, and predominance of allophycocyanin and phycoerythrin in heterotrophic cultures. The results show the effect of the variables under study for pigment production, bearing in mind the logarithmic growth phase, suggesting the possibility of producing in heterotrophic systems by Aphanothece microscópica Nageli, around 3,185 ton/year of biomass, and 3,127 kg/year of chlorophyll, 232,825 kg/year of phycocyanin, 47,775 kg/year of allophycocyanin and 7,008 kg/year of phycoerythrin, when dairy effluent is used as culture medium.

Palavras-chave : Biorefnery; chlorophyll; cyanobacteria; dairy waste; phycobiliprotein.

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