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Idesia (Arica)

versión On-line ISSN 0718-3429

Resumen

BATISTA ZONTA, João; TORRES DE SOUZA, Leandro; FERNANDES DOS SANTOS DIAS, Denise Cunha  y  MANTOVANI ALVARENGA, Eveline. COMPARING THE TETRAZOLIUM TEST METHODOLOGIES FOR SEEDS OF COFFEE SHRUB. Idesia [online]. 2009, vol.27, n.2, pp.17-23. ISSN 0718-3429.  http://dx.doi.org/10.4067/S0718-34292009000200002.

The tetrazolium test is a promising alternative for rapid evaluation of the seed viability. Two described methodologies are available for coffee shrub. Thus, this study was conducted to verify what methodology is more efficient for fast determination of both viability and energy. So, seeds from four lots were subjected to procedures as follows. 1) Soaking for 24 hours at 30 ºC; cutting the portion of the endosperm containing the embryo; exposure of this portion to 0.1% tetrazolium solution for 16 hours at 35 ºC; then, the embryo is removed with the aid of a bistoury for subsequent evaluation. 2) Soaking for 24 hours at 30 ºC; removal of the embryo and its exposure to 0.1% tetrazolium solution for 16 hours at 35 ºC for subsequent evaluation. To comparing the results, the germination test accomplished and the final percent germination and the vigor (1st germination counting) were evaluated. The methodology 2 showed to be more promising for the evaluation of both viability and vigor of coffee shrub seeds, since the obtained values were equal to those of the germination test. The methodology 1 was not efficient, as showing problems concerning to coloration of the embryos, therefore generating doubts during the evaluation of the seed viability.

Palabras clave : Viability; test fast; efficiency.

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