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Idesia (Arica)

versión On-line ISSN 0718-3429

Resumen

SESSA FIALHO, Gustavo; DOVALE, Julio César; MOREIRA SOBREIRA, Fabricio  y  ROMAIS SCHMILDT, Edilson. Behavior of Laelia tenebrosa (Orchidaceae), seedlings in vitro inoculated in different substrates. Idesia [online]. 2011, vol.29, n.1, pp.103-105. ISSN 0718-3429.  http://dx.doi.org/10.4067/S0718-34292011000100014.

This research studied in vitro development of Laelia tenebrosa Rolfe seedlings under different substrates watered with a nutrient solution. The nutrient solution was the same for all treatments: MS liquid medium + 2 mg of BAP per liter + 0.1 mg of AIA and pH adjusted to 5.8 ± 1.0 before autoclaving at 121 °C , 1 atm. for 20 minutes. The evaluated treatments were: T1 - nutrient solution + 6 g of agar per liter, T2 - nutrient solution + vermiculite and T3 - nutrient solution + rhizome fiber of Pteridium aquilinum Kuhn. The experiment was conduced in a completely randomized design, with 4 seedlings per replicate. When the seedlings reached an average height of 1 cm they were sub-cultivated in a 250 cm3 bottle containing: T1 - 50 mL nutrient solution + 6 g agar per liter; T2 - 15 g nutrient solution + vermiculite and T3 - 15 g nutrient solution + rhizome fiber of Pteridium aquilinum Kuhn. Each gram of substrate of T2 and T3 was watered with 5 ml of nutrient solution. After 180 days of seedling sub-cultivation the following parameters were evaluated: height of aerial part, leaf number and total fresh mass. There was no significant difference between the treatment means for aerial height or leaf number. However, when weight of the fresh mass was evaluated, T1 was superior to T3; it showed an increase of 58.5 mg on the average in the weight of the fresh mass of seedlings.

Palabras clave : Laelia tenebrosa Rolfe; Orchidaceae; in vitro development..

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