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Idesia (Arica)

On-line version ISSN 0718-3429


GONZALEZ-SOTO, Tania Elisa et al. Inoculation of Trichoderma longibrachiatum in transgenic cotton: change in the phenolics compounds and enzymes of oxidative stress. Idesia [online]. 2017, vol.35, n.1, pp.19-24.  Epub Apr 12, 2016. ISSN 0718-3429.

The objective of the present study was to evaluate the activity of phenylalanine ammonia-lyase (PAL), peroxidase (POX) and the production of phenolic compounds and flavonoids in transgenic cotton plants in response to inoculation with Trichoderma longibrachiatum (ICA-4). Ninety days after inoculation with T. longibrachiatum, leaves of the cotton plants were collected and the enzymes PAL, POX and phenolic compounds were determined. The results showed that inoculation increased the content of phenolic compounds and flavonoids by 18% and 45%, respectively, compared to non-inoculatedplants. The activity of the enzymes peroxidase (4.88 Ux10-5/mg protein), and phenylalanine ammonia-lyase (0.0176 U/mg protein) showed significant differences compared to the enzymatic activity of uninoculated plants (3, 48x10-5 U/mg proteinfor POX and 0.01 U/mg proteinfor PAL). These results suggest the induction of a systemic resistance response induced by seed inoculation with the Trichoderma ICA-4 strain.

Keywords : route phenylpropanoid; biocontrol; Trichoderma; enzymes of oxidative stress.

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