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International journal of odontostomatology

versión On-line ISSN 0718-381X

Resumen

RAMOS-LOPEZ, M et al. In vitro Evaluation of Four Sealer Cements Biocompatibility with Human Osteoblasts. Int. J. Odontostomat. [online]. 2019, vol.13, n.1, pp.64-68. ISSN 0718-381X.  http://dx.doi.org/10.4067/S0718-381X2019000100064.

Correct apical sealing is an important step during root canal treatment, hence, gutta-percha points and sealant are used. There are several chemical compositions on the market, so it is important to evaluate the effects of these in the periapical healing process. The aim of this study was to evaluate the biocompatibility of four sealer cements with different chemical composition placed in contact with human osteoblast. Different extracts were prepared at two concentrations (10 mg/mL and 40 mg/mL) and two exposure times (10 min and 8 h) these were placed in contact with human osteoblast to evaluate cytotoxicity and proliferation at 24, 48 and 72 h with their respective controls and blanks. A statistical analysis was performed with ANOVA of one factor and Bonferroni post hoc. Results obtained in cytotoxicity and proliferation assays, indicated that the resinbased cement is not biocompatible with osteoblast. The poly-dimethylxilosanbased cement was the only that did not show cytotoxicity at any time of exposure and concentration examined in this study.

Palabras clave : biocompatibility; sealer cements; osteoblasts.

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