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Revista chilena de cirugía

versión On-line ISSN 0718-4026

Resumen

PEREIRA, Nicolás; GONZALEZ, Esteban  y  CAVIEDES, Pablo. Cryopreservation of adipose derived stromal cells: comparison of different cryopreservation media. Rev Chil Cir [online]. 2016, vol.68, n.4, pp.295-301. ISSN 0718-4026.  http://dx.doi.org/10.1016/j.rchic.2016.03.007.

Aim To obtain stromal cells derived from adipose tissue, to measure and compare viability rates before and immediately after cryopreservation cycle, using different combinations of cryoprotective agents in order to identify the best cryopreservation medium. Material and method Viability rate after cryopreservation of stromal cells derived from adipose tissue were assessed by flow cytometry with propidium iodide. Samples of stromal cells obtained from 5 patients were kept defined, bovine serum-free media (DMEM/Ham-F12), supplemented with one of the following combinations of compounds: 10% dymethylsulfoxide (DMSO); Trehalose 10% DMSO + 7.6%; 10% DMSO + 10% human albumin and 10% DMSO + 7.6% Trehalose + 10% human albumin. Results No statistically significant differences were observed in the viability rates of stromal cells derived from adipose tissue after a cryopreservation cycle. However, we observed a tendency towards improvement of recovery rate when human albumin was added to the medium. Conclusions None of the studied conditions proved superior to others in terms of cell vitality after a cryopreservation cycle. Hence, we conclude that the cryopreservation of stromal cells derived from adipose tissue in an environment that combines DMEM/F12 with 10% DMSO + 7.6% Trehalose + human albumin 10% does not achieve a significantly higher recovery rate than only frozen solely with DMSO 10%.

Palabras clave : Cryopreservation; Adipose derived stromal cells; Stem cells.

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