SciELO - Scientific Electronic Library Online

 
vol.41 issue5Effects of temperature and salinity on growth and survival of the Pacific red snapper Lutjanusperu (Pisces: Lutjanidae) juvenileIntertidal copepods (Crustacea) in Chilean oceanic islands: a standpoint by null models and island biogeography author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Latin american journal of aquatic research

On-line version ISSN 0718-560X

Abstract

SPANO, Carlos  and  FLORES, Verónica. Staining protocol for the histological study of sea anemones (Anthozoa: Actiniaria) with recommendations for anesthesia and fixation of specimens. Lat. Am. J. Aquat. Res. [online]. 2013, vol.41, n.5, pp.1019-1024. ISSN 0718-560X.  http://dx.doi.org/103856/vol41-issue5-fulltext-23.

Many of the characteristics used in sea anemone taxonomy can only be examined through histological sections. Since there is no standardized procedure for this purpose, various anesthesia and fixation techniques applied to specimens of the intertidal species Anthopleura hermaphroditica and Bunodactis hermafroditica are discussed. Additionally, further modifications are proposed to the Masson's trichrome method according to the results obtained on these species. The combined effect of the short application of menthol crystals, together with small doses of MgCl2 were the most satisfactory anesthetics for maintaining the specimens expanded. The best preparations were obtained from samples fixed for several months in 8% seawater formalin; however, in order to achieve a good differentiation of the tissue, mordanting the samples with Bouin's fixative was necessary. Besides being a fast method, the modified Masson's trichrome gives very good contrasts between the epithelia and the mesoglea, and allows controlling the timing of differentiation during staining. The present paper includes suggestions and precautions and thus offers practical help for the histological study of sea anemones.

Keywords : Actiniidae; Anthopleura; Bunodactis; Masson's trichrome; histology.

        · abstract in Spanish     · text in English     · English ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License