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Latin american journal of aquatic research

versión On-line ISSN 0718-560X

Resumen

AGUILERA, Victor M; ESCRIBANO, Rubén  y  MARTINEZ-OYANEDEL, José. Electrophoretic protein profiles of mid-sized copepod Calanoides patagoniensis steadily fed bloom-forming diatoms. Lat. Am. J. Aquat. Res. [online]. 2015, vol.43, n.4, pp.798-806. ISSN 0718-560X.  http://dx.doi.org/10.3856/vol43-issue4-fulltext-20.

Recent field and experimental evidence collected in the southern upwelling region off Concepción (36°5'S, 73°3'W) showed an abrupt reduction (<72 h) in the egg production rates (EPR) of copepods when they were fed steadily and solely with the local bloom-forming diatom Thalassiosira rotula. Because diatoms were biochemically similar to dinoflagellate Prorocentrum minimum, a diet which supported higher reproductive outcomes, the fecundity reduction observed in copepod females fed with the diatom may have obeyed to post-ingestive processes, giving rise to resources reallocation. This hypothesis was tested by comparing feeding (clearance and ingestion rates), reproduction (EPR and hatching success) and the structure of protein profiles (i.e., number and intensity of electrophoretic bands) of copepods (adults and eggs) incubated during 96 h with the two food conditions. The structure of protein profiles included molecular sizes that were calculated from the relative mobility of protein standards against the logarithm of their molecular sizes. After assessing the experimental conditions, feeding decreased over time for those females fed with T. rotula, while reproduction was higher in females fed with P. minimum. Electrophoretic profiles resulted similar mostly at a banding region of 100 to 89-kDa, while they showed partial differences around the region of 56-kDa band, especially in those females fed and eggs produced with T. rotula. Due to reproductive volume was impacted while larvae viability, a physiological processes with specific and high nutritional requirements, was independent on food type; post-ingestive processes, such as expression of stress-related proteins deviating resources to metabolic processes others than reproduction, are discussed under framework of nutritional-toxic mechanisms mediating copepod-diatoms relationships in productive upwelling areas.

Palabras clave : diatoms; blooms; food; copepods; reproduction; protein profiles.

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