SciELO - Scientific Electronic Library Online

 
vol.44 número4Nuevos registros y datos biológicos de Lolliguncula (Lolliguncula) argus Brakoniecki & Roper, 1985 (Myopsida: Loliginidae) en el Golfo de Tehuantepec, MéxicoEvaluación de la eficiencia productiva de tres líneas de tilapia con reversión sexual en un sistema de recirculación (RAS) índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

  • En proceso de indezaciónCitado por Google
  • No hay articulos similaresSimilares en SciELO
  • En proceso de indezaciónSimilares en Google

Compartir


Latin american journal of aquatic research

versión On-line ISSN 0718-560X

Resumen

TORRES, Pamela et al. Genotyping and host-specific relationship of infectious pancreatic necrosis virus in Chile. Lat. Am. J. Aquat. Res. [online]. 2016, vol.44, n.4, pp.860-868. ISSN 0718-560X.  http://dx.doi.org/10.3856/vol44-issue4-fulltext-23.

Samples of fish organs (kidney and spleen) from the main salmonid species farmed in Chile were analyzed through the nested polymerase chain reaction (nested PCR) to detect the presence of infectious pancreatic necrosis virus (IPNV). The technique proved to be efficient and sensitive, allowing amplification of viral genetic material for sequencing directly from tissue, even from samples that could not be isolated in cell culture. The phylogenetic analysis showed the two genogroups previously described in the country, i.e., European (Genogroup 5) and American (Genogroup 1), being the IPNV that belong to Genogroup 5 the dominant one (78.8%). It is clear that the Chilean IPN viruses are clustered within the Genogroup 1 forming a sub-group that is separated from the reference strains (e.g., WB, VR-299), hence we propose its denomination as a Chilean genotype. Additionally, a statistically significant host-specific relationship was observed between the genogroups identified: viruses from Genogroup 5 were detected in Salmo salar, while the ones from Genogroup 1 were detected in Oncorhynchus mykiss or O. kisutch (P < 0.001). The association of this host-specific relationship with the virulence can provide important information for the management and control of IPNV in Chile.

Palabras clave : virus; IPNV; PCR anidado; genotipificación; filogenia; relación hospedador-específica; gen VP2; virus; IPNV; nested PCR; genotyping; phylogeny; host-specific relation; VP2 gene.

        · resumen en Español     · texto en Español     · Español ( pdf )

 

Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons