Resumen

ARANEDA, Cristian et al. Utility of five SSR markers for genetic diversity and paternity exclusion analysis in the Patagonian toothfish. Lat. Am. J. Aquat. Res. [online]. 2017, vol.45, n.1, pp.188-192. ISSN 0718-560X.  http://dx.doi.org/10.3856/vol45-issue1-fulltext-18.

The Patagonian toothfish or Chilean sea bass (Dissostichus eleginoides), found in the Southern Ocean surrounding Antarctica, is an important fishery species for Chile. This high-value species is regarded as overfished, making it an attractive target for aquaculture. When developing a reproduction program for any aquaculture species, it is important to implement genetic tools to evaluate diversity, inbreeding, and parentage. We calculated genetic diversity and paternity/maternity exclusion probabilities based on five commonly-used microsatellite loci in a natural population of Patagonian toothfish from southern Chile (n = 34) in order to evaluate the potential utility of these five markers in stock management. The observed number of alleles per locus (N_a) and observed heterozygosities (H_O) are within range as described by studies performed in other sub-Antarctic regions. All five loci were strongly polymorphic, with H_O > 0.6 and N_a > 7, and paternity exclusion probabilities were high (PE > 0.99), indicating the potential utility of these loci in paternity/maternity exclusion analyses of Patagonian toothfish.

Palabras clave : Dissostichus eleginoides; Chilean sea bass; heterozygosity; microsatellites; genetic diversity.

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