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Latin american journal of aquatic research

versión On-line ISSN 0718-560X


JIMENEZ-REYES, María Fernanda; YANY, Gabriel  y  ROMERO, Jaime. Protocol for obtaining rainbow trout (Oncorhynchus mykiss) axenic fingerlings. Lat. Am. J. Aquat. Res. [online]. 2017, vol.45, n.5, pp.1064-1069. ISSN 0718-560X.

In this paper a protocol based on the application of Buffodine® disinfectant and a mix of antibacterials for obtaining rainbow trout (Oncorhynchus mykiss) axenic fingerlings is described. This allowed to obtain and maintain axenic trout fingerlings for a period of 26 days. This time allow to achieve a gnotobiotic organism, a fundamental tool in studies of the understanding bacteria-host interaction or host-parasite interaction.

Palabras clave : Oncorhynchus mykiss; gnotobiotic; axenic; fingerling; disinfectant; antibacterial.

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