SciELO - Scientific Electronic Library Online

 
vol.69 issue1Comparison of Root Induction in Mature Filbert (Corylus avellana L.) Explants by Agrobacterium Rhizogenes and Indolbutiric Acid author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Chilean journal of agricultural research

On-line version ISSN 0718-5839

Abstract

CARTES R, Priscila et al. Encapsulated Somatic Embryos and Zygotic Embryos for Obtaining Artificial Seeds of Rauli-Beech (Nothofagus alpina (Poepp. & Endl.) Oerst.). Chilean J. Agric. Res. [online]. 2009, vol.69, n.1, pp.112-118. ISSN 0718-5839.  http://dx.doi.org/10.4067/S0718-58392009000100014.

Somatic and zygotic embryos from mature seeds of rauli-beech, Nothofagus alpina (Poepp. & Endl.) Oerst., were encapsulated in different artificial endosperms in order to generate a cover that fulfills the function of nourishment and protection of the embryos, facilitating their later germination. The content of sodium alginate varied by 4%, 3%, and 2%, as did the immersion time in calcium chloride (CaCl2), which acts as complexing agent. The artificial endosperm components of the Murashige and Skoog medium (MS) were added, supplemented with 0.5 mg L-1 indolacetic acid (IAA), 0.5 mg L-1 naphthaleneacetic acid (NAA), 2 mg L-1 6-benzylaminopurine (BAP) and 30 g L-1 sucrose. The germinative behaviors of encapsulated somatic and zygotic embryos were evaluated after 4 wk. Comparing the percentages of germination reached by encapsulated somatic and zygotic embryos it was observed that they had similar germinative behavior according to the type of encapsulation applied. However, zygotic embryos substantially exceeded the germination levels reached by somatic embryos, 100% vs. 45% respectively.

Keywords : rauli-beech; somatic embryogenesis; synthetic seed; sodium alginate.

        · abstract in Spanish     · text in English     · English ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License