SciELO - Scientific Electronic Library Online

 
vol.71 número1Inducción de resistencia en garbanzo (Cicer arietinum L.) contra Ascochyta rabiei por la aplicación de productos químicos y extractos vegetalesComposición química, actividades insecticida y antifúngica de aceites esenciales de frutos de tres especies Zanthoxylum de Colombia índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

Compartir


Chilean journal of agricultural research

versión On-line ISSN 0718-5839

Resumen

ARAYA, Carolina et al. Symptom severity and viral protein or RNA accumulation in lettuce affected by big-vein disease. Chilean J. Agric. Res. [online]. 2011, vol.71, n.1, pp.63-72. ISSN 0718-5839.  http://dx.doi.org/10.4067/S0718-58392011000100008.

Big-vein disease (BVD) is a widespread and economically damaging disease in lettuce (Lactuca sativa L.). Typical symptoms are chlorotic clearing around leaf veins, leaf deformations, and impaired head development. In this research, we studied the relationship between symptom intensity and protein and viral RNA accumulation in infected plants. Naturally infected lettuce plants, from the field or greenhouse, were classified according to their symptomatology:  mild, moderate, severe, and symptomless. Coat protein accumulation was evaluated by a double antibody sandwich/enzyme-linked immunosorbent assay (DAS-ELISA), and RNA levels were studied by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and quantitative RT-PCR. Virus coat protein accumulation did not differ for the two viruses associated with this disease among lettuce plants showing different symptom severity. Similarly, abundance of Mirafiori lettuce big-vein virus (MLBVV)-RNA3 or Lettuce big-vein associated virus (LBVaV)-RNA-2 were not different (P > 0.05) for diverse big vein disease severity rating scales. This suggests that symptom severity expressed by big-vein diseased lettuce plants did not necessarily reflect the accumulation of viruses associated with this disease in the host. Therefore, lettuce plants showing mild symptoms of BVD do not necessarily present lower virus levels than plants showing more severe symptomatology.

Palabras clave : Lettuce big-vein disease; symptom expression.

        · resumen en Español     · texto en Inglés     · Inglés ( pdf )

 

Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons