SciELO - Scientific Electronic Library Online

 
vol.15 número2Improving selenium status in plant nutrition and qualityRhizosphere-induced heavy metal(loid) transformation in relation to bioavailability and remediation índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

Links relacionados

  • Em processo de indexaçãoCitado por Google
  • Não possue artigos similaresSimilares em SciELO
  • Em processo de indexaçãoSimilares em Google

Compartilhar


Journal of soil science and plant nutrition

versão On-line ISSN 0718-9516

Resumo

LAGOS, L et al. Current overview on the study of bacteria in the rhizosphere by modern molecular techniques: a mini‒review. J. Soil Sci. Plant Nutr. [online]. 2015, vol.15, n.2, pp.504-523.  Epub 30-Abr-2015. ISSN 0718-9516.  http://dx.doi.org/10.4067/S0718-95162015005000042.

The rhizosphere (soil zone influenced by roots) is a complex environment that harbors diverse bacterial populations, which have an important role in biogeochemical cycling of organic matter and mineral nutrients. Nevertheless, our knowledge of the ecology and role of these bacteria in the rhizosphere is very limited, particularly regarding how indigenous bacteria are able to communicate, colonize root environments, and compete along the rhizosphere microsites. In recent decades, the development and improvement of molecular techniques have provided more accurate knowledge of bacteria in their natural environment, refining microbial ecology and generating new questions about the roles and functions of bacteria in the rhizosphere. Recently, advances in soil post‒genomic techniques (metagenomics, metaproteomics and metatranscriptomics) are being applied to improve our understanding of the microbial communities at a higher resolution. Moreover, advantages and limitations of classical and post‒genomic techniques must be considered when studying bacteria in the rhizosphere. This review provides an overview of the current knowledge on the study of bacterial community in the rhizosphere by using modern molecular techniques, describing the bias of classical molecular techniques, next generation sequencing platforms and post‒genomics techniques.

Palavras-chave : Microbial ecology; molecular techniques; rhizosphere; next‒generation sequencing.

        · texto em Inglês     · Inglês ( pdf )

 

Creative Commons License Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons