SciELO - Scientific Electronic Library Online

 
vol.15 número3Effects of irrigation with treated agro-industrial wastewater on soil chemical characteristics and fungal populations during processing tomato crop cycleEvaluation of the phosphate solubilization potential of Trichoderma strains (Trichoplus JCO) and effects on rice biomass índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

  • En proceso de indezaciónCitado por Google
  • No hay articulos similaresSimilares en SciELO
  • En proceso de indezaciónSimilares en Google

Compartir


Journal of soil science and plant nutrition

versión On-line ISSN 0718-9516

Resumen

PADMAVATHI, T. Optimization of phosphate solubilization by Aspergillus niger using plackett-burman and response surface methodology. J. Soil Sci. Plant Nutr. [online]. 2015, vol.15, n.3, pp.781-793. ISSN 0718-9516.  http://dx.doi.org/10.4067/S0718-95162015005000053.

Phosphorus is one of the major components required for the metabolic activities and for the growth of any organism. Many soil organisms are known to solubilize inorganic phosphates. Aspergillus niger isolated from the soil showed extensive solubilization of Tri-calcium phosphate.It was observed that the solubilization was due to drop in pH.Acidification was due to production of organic acids by the fungi. The effect of different medium components on the solubilization of phosphate was determined using the Packett-Burman design. It was observed that glucose and ammonium sulphate had significant effect on phosphate solubilization.Considering the Plackett-Burman results, the response surface methodology was used for optimization of these medium components along with tri-calcium phosphate on P-solublization. The analysis from RSM revealed that the optimum values for the tested variables were glucose - 2g/50ml, ammonium sulphate -0.2g/50ml and tri-calcium phosphate - 1g/50ml. Phosphate solubilization of 3.64 mg/ml was observed as comparison to original level of 1.88mg/ml, which was a 1.93-fold increase was obtained. From the HPLC analysis it was observed that oxalic acid and lactic acid were the major acids responsible for enhancing the P solubilization.

Palabras clave : Phosphate solubilizing fungi; Plackett-Burman; response surface methodology; organic acids.

        · texto en Inglés     · Inglés ( pdf )

 

Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons