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Revista chilena de historia natural

Print version ISSN 0716-078X

Rev. chil. hist. nat. vol.86 no.4 Santiago Dec. 2013

http://dx.doi.org/10.4067/S0716-078X2013000400012 

NATURAL HISTORY NOTE

 

First record of the invasive algae Didymosphenia geminata in the Lake Nahuel Huapi: Argentina, Patagonia

Primera detección del alga invasiva Didymosphenia geminata en el lago Nahuel Huapi: Argentina, Patagonia

 

GUADALUPE BEAMUD*, GUSTAVO BAFFICO, FERNANDO PEDROZO & MÓNICA DIAZ

Grupo de Calidad de Aguas y Recursos Acuáticos (GECARA), Instituto de Biodiversidad y Medio Ambiente (INIBIOMA, CONICET - Universidad Nacional del Comahue), Quintral 1250, (8400) San Carlos de Bariloche, Río Negro, Argentina
*Corresponding author: beamudsg@comahue-conicet.gob.ar


 

The benthic diatom Didymosphenia geminata (Lyngbye) M. Schmidt is a very aggressive invasive species found in rivers and streams in different parts of the world. It has become a major concern for its tendency to form conspicuous blooms in oligotrophic aquatic systems with potential for detrimental impacts on recreational fishing.

The first blooms of D. geminata in South America were found in May 2010 in the River Futaleufú (Los Lagos Region, Chile) (CIEP 2010). Subsequently, the species was detected upstream, in the River Futaleufú (Chubut Province, Argentina), in August 2010 (Sastre et al. 2013). In November 2011 the diatom was recorded in the River Manso (Río Negro Province, Argentina) (M Alemanni, comm. pers. 2011), and in Chimehuin and Collon Cura rivers (Neuquén Province, Argentina) (Abelli Bonardi et al. 20121). Given its explosive development and the notable appearance in Lake Nahuel Huapi during the austral summer of 2013, our aim was to document the first record for a large (557 km2) lentic waterbody in the Andean Patagonia region.

On January 29, 2013, samples were collected at three sites spaced 20 m apart along the shoreline of Lake Nahuel Huapi (41°06' 20.1'' S, 71°26' 9.6" W, Fig. 1) in the area known as Bahía Serena (BS 1 and BS 2), and from a small stream that connects a wetland ("Mallín") with Lake Nahuel Huapi. Water temperature, pH (Orion 3Star pH meter) and electrical conductivity (ORION 3Star) were determined in situ. Samples for water chemistry were collected at 0.2 m depth and were stored in cleaned plastic bottles. The samples were transported to the laboratory where the following determinations were performed using methods outlined by APHA (1992): silicate (SiO2), total iron (total Fe), sulphate (SO4=), total phosphorus (TP), soluble reactive phosphorus (SRP), nitrate (NO3-), and total nitrogen (TN). The total organic phosphorus (TOP) was estimated as the difference between TP and SRP. The N:P ratio was calculated as TN:TP. The Si:P was calculated as the ratio of the Si-SiO2 and SRP. Algal samples were collected directly from rock substrates with a spatula and placed in plastic vials. Immediately on arrival in the laboratory, subsamples were mounted on slides for identification under a microscope (Olympus BX51) at x1000 magnification. The maximum linear dimension (MLD) of the cells was determined according to Reynolds (2006).

 

Fig. 1: Map of Northern Patagonia showing sites where D. geminata was found. 1: River Futaleufú (Chile), 2: River Futaleufú (Argentina), 3: River Manso (Argentina), 4: River Collon Cura (Argentina), 5: River Chimehuin (Argentina), NH: Lake Nahuel Huapi (Argentina, this study).

Mapa de Patagonia Norte mostrando los sitios donde se encontró D. geminata. 1: Río Futaleufú (Chile), 2: Río Futaleufú (Argentina), 3: Río Manso (Argentina), 4: Río Collon Cura (Argentina), 5: Río Chimehuin (Argentina), NH: Lago Nahuel Huapi (Argentina, este estudio).

Didymosphenia geminata bloomed on the shore of Lake Nahuel Huapi in the public beach at Bahía Serena area (Fig. 2). The littoral zone has two different areas: one is an entirely volcanic bed-rock (Fig. 2A-B) and the other one is composed of sand and cobbles. The species was detected only in the lake and not in the effluent of the wetland (Mallín). The average (± standard deviation) MLD of D. geminata cells in Lake Nahuel Huapi samples was 120 urn (± 12.7 μm SD) and the average width measured was 37.5 μm (± 3.5 μm SD) (Fig. 2C). The stalks of D. geminata were profusely covered by small diatoms, mainly Achnanthidium sp. but also Navicula sp. and Tabellaria sp. The morphology of the cells of D. geminata found in Bahía Serena is consistent with that described by other authors as Sastre et al. (2013) in samples from River Futaleufú in the province of Chubut, and Kilroy (2004) in rivers of the South Island of New Zealand, among others.

 

Fig. 2: (A) Detail of the proliferation of D. geminata, (B) Close up of the development of D. geminata on stones, (C) D. geminata cell. Scale bar = 10 μm.

(A) Detalle de la proliferación de D. geminata, (B) Primer plano del desarrollo de D. geminata sobre las piedras, (C) célula de D. geminata. Escala = 10 μm.

The pH of the sampling sites was neutral and conductivity and P were both low (Table 1). Total organic P, was higher than the inorganic fraction. N concentrations (dissolved and total) were higher than P and SiO2 values. Sulphate concentrations and total Fe were near or below the detection limit of the methods. The effluent from Mallín had higher salt content and nutrients than the lake (Table 1). Lake Nahuel Huapi is in the headwaters of an important hydrological catchment of 4260 km2 and is a waterbody that has been described as ultraoligotrophic (Diaz et al. 2007), a characterization that can be applied broadly to the lake. Because this waterbody is located in a National Park, the uses of the water are mainly recreational. Sport fishing is an important economic activity in the region. River Limay originates in Lake Nahuel Huapi, and 5 reservoirs built along its watercourse, are used for electrical generation, irrigation, recreational purposes, sport fishing and drinking water.

TABLE 1

Environmental conditions of Lake Nahuel Huapi in the coastal area of Bahía Serena (BS1 and BS2) and Mallín effluent at the sampling date.

Condiciones ambientales para la fecha de muestreo en dos sitios de la zona costera de Bahía Serena (BS1 y BS2) y en el efluente del Mallín en el Lago Nahuel Huapi

 

The presence of Didymosphenia geminata in lakes is little known. The species have been described in detail for Lake Baikal (Whitton et al. 2009) and also part of the epilithic community of lentic environments in Turkey (Sahin 2000), Russia (Medvedeva 2001) and the lake District in England (Godward 1937 in Whitton et al. 2009). Our identification in the Lake Nahuel Huapi is the first confirmation of D. geminata blooms for a South American lake. The first blooms of D. geminata in rivers of South America were reported in Chile's Futaleufú watershed. Likewise the first bloom found in Patagonia Argentina occurred in the River Futaleufú. These watersheds including Lake Nahuel Huapi are located along a 600 km North-South axis of Patagonia (Fig. 1) and have similar chemical, physical and biological characteristics (Diaz et al. 2007). The pH, nutrients (Phosphorus and Nitrogen) and the N:P ratio of Lake Nahuel Huapi agree with those reported by Reid & Torres (2013) for the Baker and Aysen Rivers (Chile), where D. geminata mats occur. These Chilean rivers are also oligotrophic, with neutral pH (7.05-7.8) and low conductivity (34.3-62.3 µS cm-1) (Reid & Torres 2013). They also share environmental characteristics with other rivers, where massive blooms of this species have also been detected, such as rivers in New Zealand. In these rivers, the conductivity varied over a wide range, between 29.8 and 1,612 µS cm-1 and the ratio DIN: inorganic P between 3.7 and 98.0 (Larned et al. 2007). Furthermore, in lakes of Turkey with pH values ranging from 7.03-7.15 and values of DIN less than 53 µg N l-1 (Sahin 2000), D. geminata was present in the environment without forming blooms. On Lake Nahuel Huapi, the Si:P ratio was high, situation in which diatoms are more efficient and have a competitive advantage compared to other algal groups (Hecky & Kilham 1988). The success of the initial colonization of D. geminata and the potential for future blooms is defined not only by the introduction of the cells, but also by potential controls that define a suitable window habitat for survival (Cullis et al. 2012). Such controls include: high light availability (Whitton et al. 2009), low nutrient concentrations (Spaulding & Elwell 2007), and low temperatures (Kumar et al. 2009). The chemical characteristics of Lake Nahuel Huapi and the rivers of Patagonia where D. geminata has been found correspond to potential controls that define the window habitat. Thus, the study of these environments and distribution of D. geminata should be a key element of the research efforts in the future to predict the possible occurrence of massive blooms.

ACKNOWLEDGMENTS: This study was supported by funds from the Universidad Nacional del Comahue (Programme 04B166) to Dr. Mónica M. Diaz. We are very grateful to Max Bothwell for the English improvement of the manuscript.

NOTE

1 ABELLI BONARDI M, G BAFFICO, G BEAMUD, L CASTIÑEIRA, DÍAZ M, LAFFITTE L, LAUREN-ZANO B, MORZENTI P, MUÑIZ SAAVEDRA J, PEDROZO F, RIVERA D & P TEMPORETTI (2012a) Programa de vigilancia, seguimiento y control de Didymosphenia geminata (Lyngbye) Schmidt 1899 en la Provincia del Neuquén. 5th Argentinean Congress of Limnology, November 2012, Santa Fé, Argentina.

 

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Editorial responsibility: Bernardo Broitman
Received September 6, 2013; accepted October 21, 2013

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