SciELO - Scientific Electronic Library Online

vol.35 issue2Cytohesins and centaurins control subcellular trafficking of macromolecular signaling complexes: Regulation by phosphoinositides and ADP-Ribosylation FactorsWnt signaling: A complex issue author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Biological Research

Print version ISSN 0716-9760

Biol. Res. vol.35 no.2 Santiago  2002 

Biol Res 35: 267-275, 2002

Extracellular signals, cell interactions and transcription
factors involved in the induction of the neural crest cells


Millennium Nucleus in Developmental Biology, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile. 1: Both authors contributed equally to this work


The neural crest is induced at the border between the neural plate and the epidermis. A complex set of signals is required for the specification of the crest cells between the epidermis and the neural plate. Here we discuss evidence supporting a model for neural crest induction in which different signals contribute in a sequential order. First, a gradient of bone morphogenic proteins (BMPs) is established in the ectoderm that results in segreggation into neural plate, neural folds and epidermis at increasing levels of BMP activity. Thus, the neural folds are induced at a precise threshold concentration of BMP, but this neural fold has an anterior character. In a second step, these anterior neural folds are transformed into prospective neural crest by posteriorizing signals due to fibroblast growth factor, Wnts and retinoic acid. Finally, the induced cells interact to complete neural crest induction by a process that requires Notch/Delta signaling. Once neural crest formation has been induced by this combination of extracellular and intracellular signals, a cascade of transcription factors is activated in these cells that culminates in the ultimate steps of neural crest differentiation.

Key terms: neural crest, induction, BMPs, Wnts, FGF, retinoic acid, Notch, delta


The neural crest was discovered by His in 1868 and has since remained a focus of intense research in Developmental Biology. The neural crest is comprised of a very particular set of cells, which segregate from the dorsal portion of the neural tube and migrate through the embryo to generate a prodigious array of cell types, including neurons, support cells for the peripheral nervous system, pigment cells, smooth muscle, cranofacial cartilage, as well as bone and fin in amphibian and fishes (for a review on neural crest cells see: Le Douarin and Kalcheim, 1999; La Bonne and Bronner-Fraser, 2000; Aybar and Mayor, 2002)

Much of the work discussed here focuses on neural crest development in Xenopus laevis embryos, where detailed knowledge of cellular and molecular aspects of the early development process is available. Results from work on Xenopus embryos has contributed substantially to our current understanding of the inductive process, both in terms of the cellular and molecular interactions involved. More recently, an increasing number of genes expressed in the neural crest of this species have been identified. In this review, we will analyze certain aspects concerning the induction of the neural crest in Xenopus, chicken and zebrafish. Also, we will discuss the role of different diffusible and membrane bound molecules in activation of the genetic program required for neural crest specification.

A double gradient Model of neural crest induction

Experiments in Xenopus and zebrafish support a BMP gradient model for neural crest induction (LaBonne and Bronner-Fraser, 1998; Nguyen et al., 1998; Morgan and Sargent, 1997; Marchant et al., 1998; Barth et al., 1999, Mayor et al., 2001). However, recent reports in Xenopus indicated that induction of neural crest markers could not be explained as the simple consequence of lowering BMP activity in the ectoderm, thereby pointing to the need to identify additional signals (LaBonne and Bronner-Fraser, 1998; Wilson et al., 1997). Some of these have now been identified and include fibroblast growth factor (FGF), Wnts, and retinoic acid (RA) (LaBonne and Bronner-Fraser, 1998; Villanueva et al., 2002). In Xenopus, these molecules function, at least in part, as posteriorizing signals that pattern the anterior-posterior axis of the neural fold (Villanueva et al., 2002).

We propose the following model for neural crest induction. Initial neural crest induction is dependent on a gradient of BMP activity established in the ectoderm. The neural plate border, induced at a precise location within the medio-lateral axis of the ectoderm, has anterior character. At a later stage, but still during the early neural crest induction phase, posteriorizing signals originating from the posterior part of the embryo transform a region of the previously induced anterior neural plate border into prospective neural crest cells. These signals could correspond to Wnts, FGFs and RA, some of which are expressed at the correct time and place to act as posteriorizing agents in Xenopus and zebrafish (Lekven et al., 2001; Erter et al., 2001; Kiecker and Niehr, 2001). The anterior neural plate border either does not receive such signals or these are inhibited by other agents produced by the anterior regions of the embryo, such as cerberus, Dkk1 or Dan (Bowmeester et al., 1996; Kasanskaya et al., 2000; Hashimoto et al., 2000; Ogita et al., 2001), three known Wnts inhibitors (for a discussion of Wnt signaling pathways, see Hendriks and Reichmann, 2002). As a consequence, this region does not develop into neural crest. As neurulation proceeds and the neural plate folds into a tube, molecules such as BMPs, Wnts and possibly FGF and RA are expressed in the epidermis and/or in the dorsal part of the neural tube, including the neural crest, where they are required for late induction (Liem et al., 1995; Villanueva et al., 2002; Lekven et al., 2001; Wilson et al., 2001; Jing et al., 2001; Dorsky et al., 1998; Chang et al., 1998; Ikeya et al., 1997). New elements of the Wnt pathway, such as Xfz3 and Kermit, have been shown to be important in neural crest specification and differentiation (Deardorff et al., 2001; Tan et al., 2001). Strong evidence in favor of such a late induction step is available in the chick model, where neural tube cells analyzed at later stages can be transformed into neural crest tissue by the addition of BMPs (Liem et al., 1995; Selleck et al., 1998).

Once the neural plate border has been specifed as prospective neural crest by the signals mentioned, additional intercellular signals are required to complete the induction of the neural crest. Some of these signals depend on the interaction of Notch/Delta.

Notch/Delta signaling pathway

Notch/Delta signaling pathway has been conserved through evolution from nematodes to human. This pathway is involved in local cell-cell inductive processes that specify embryonic cell fates, in particular in the neurogenic region of the blastoderm, in the developing nervous system, and in the compartment boundaries of the Drosophila imaginal discs (Coffman et al., 1990). Also a role during vertebrate development, in processes such as somitogenesis, selection of neuronal precursors in the central nervous system and glial to neuronal transition in the developing retina is well documented (Christ et al., 2000; Baker, 2000; Scheer et al., 2001). Notch and Delta genes code for single membrane-spanning proteins, Notch being the receptor and Delta the ligand of this signaling pathway. It should be mentioned that another ligand for Notch called Serrate exists, which seems to elicit similar responses (Kiyota et al., 2001). Both Notch ligand proteins have extracellular EGF repeats domain which mediate the interaction. In order to be effective, the interaction between Notch and Delta proteins must occur by contact between adjacent cells. If Notch and Delta are expressed on the same cell, an interaction is possible but does not lead to Notch activation. Additionally, Notch requires a co-receptor, called Fringe (or its vertebrate homologue Lunatic Fringe). This protein is suggested to be a glycosyltransferase that modifies the extracellular domain of Notch and thereby permits correct binding of Delta. In addition to these components, Notch undergoes at least three proteolytic events during maturation and signal transduction. First, the protease Furin cleaves Notch at a site in the extracellular domain (site 1) during transport of the receptor to the cell surface; the resulting amino-terminal domain remains associated with a carboxy-terminal transmembrane domain. Second, ligand binding triggers an additional cleavage of the extracellular region of the carboxy terminal domain (site 2), probably by a protease of the ADAM family. This second cleavage step shortens the extracellular region to 12 amino acids. Third, proteolytic cleavage within the transmembrane domain (site 3) releases the intracellular domain (ICD) so that it can translocate to the nucleus and activate transcription of target genes (Struhl and Adachi, 2000; Struhl and Greenwald, 2001). The intracellular domain of Notch poses ankyrin repeats, which behave as transcriptional activators upon interaction with the transcription factor Suppressor of Hairless which is normally found associated with the transcriptional repressor Groucho. The complex Groucho-Suppressor of Hairless represses the activity of several target genes, among them the genes of the Enhancer of Split complex (E(spl), Jennings et al., 1994). Translocation to the nucleus of the intracellular domain of Notch displaces Groucho and transforms the repressor complex to an activator complex, thereby promoting expression of the E(spl) complex genes. As mentioned before, components of this cell-cell signaling pathway have been described in vertebrates and their roles have been characterized in mouse, chicken, zebrafish and Xenopus. Although, the main function attributed to this signaling pathway in vertebrate models has been selection of neuronal precursors in the central nervous system, more recent studies have additionally unraveled a role for the Notch pathway in gliogenesis of the retina (Scheer et al., 2001) and acquisition of glial fate by neural crest stem cells upon transient activation of this pathway (Morrison et al., 2000). These studies suggest that Notch activity maintains cells in an uncommitted state and view Notch as also being part of the gliogenic instructive program. In addition, studies in zebrafish and chicken have implicated the Notch signaling pathway in neural crest development.

Notch/Delta signaling in neural crest induction

In zebrafish, attention has centered on the function of Notch signaling in differentiation of the neural crest cells. In a Delta A mutant fish, Cornell and Eisen (2002) have shown that the population of Rohon-Beard neurons, a group of cells that arise in the most dorsal region of the neural tube, expands at expense of the neural crest cells. Therefore, the activation of Notch favors neural crest cell development and is required to restrict the Rohon-Beard population (Cornell and Eisen, 2002). This study was extended by using specific morpholinos, which block the transcription of down stream genes, to demonstrate that the proneural gene Neurogenin is an essential element required for Rohon-Beard development and inhibition of neural crest fate. In summary, Notch signaling represses Neurogenin that in turn represses neural crest fate (Cornell and Eisen, 2002). Alternatively, inhibition of Notch activity (in a Delta A minus fish) promotes Neurogenin expression and Rohon-Beard development.

In chicken, neural crest cells are induced at the junction of the neural plate and the epidermal ectoderm. The current model of chicken neural crest induction involves interaction between the neural plate and the epidermis. This event induces Bmp-4 transcription at the border of the neural plate, thereby inducing neural crest in this region. In a recent paper, Endo and colleagues have shown that at the time when neural crest cells are induced Delta1 (a Notch ligand) is expressed in the epidermis and prevents Notch1 expression in the neural folds. The contact produced by the cells expressing Notch and Delta1 is able to induce the expression of Bmp-4 in a row of cells in the epidermis and represses Slug expression in the same cells. In this manner, Notch activation promotes the formation of neural crest cells through BMP-4 induction and thereby specifies sites at which neural crest cells are induced by suppression of Slug transcription (Endo et al., 2002). However, this model does not account of all the experimental data. First, previous results in chicken have shown that overexpression of Lunatic Fringe, the Notch co-receptor, leads to excessive proliferation of neural crest cells (Nellemann et al., 2001). Second, and more importantly, overexpression of the intracellular domain of Notch, enhances Notch activity, and represses Bmp-4 transcription (Endo et al., 2002).

As in other vertebrates, activation of the Notch signaling pathway in Xenopus promotes the selection of neuronal precursors within the central nervous system (Coffman et al., 1990) and participates in somitogenesis. By the time the neural crest cells are induced, Notch1 is expressed homogeneously in the neural plate and probably in part of the neural folds. On the other hand, Delta1 and Serrate are expressed in a dynamic pattern surrounding the anterior part of the prospective domain where the neural crest is induced. Interestingly, Hairy 2A, one of the genes that belongs to the vertebrate Enhancer of Split complex and lies downstream of Notch, is expressed in the neural folds including the territory where the neural crest develop. This expression profile strongly suggests a role for Notch signaling in Xenopus neural crest development. Our experiments demonstrate that Notch signaling activates and is necessary for Xslug, as well as Xmsx1 expression, a target gene of the BMP4 signaling pathway. In addition, chicken Notch activity is required for Bmp-4 expression and, in Zebrafish, the Notch signaling pathway participates in differentiation of Rohon-Beard neurons where it controls Neurogenin expression (Silva et al., 2001; Glavic, unpublished results). Thus, in Xenopus the Notch signaling pathway is involved in early stages of neural crest development and later on in neural crest differentiation. In this manner, local cell-cell interactions through the Notch/Delta pathway help define the complex genetic program required for formation of neural crest cells.

Transcriptional activity in the neural crest during induction

A number of genes that are expressed at the neural plate border have been identified in the recent years. These genes are expressed at different stages of neural crest development, in the prospective neural crest, in migratory neural crest or during their differentiation. In this section, we will briefly discuss how gene expression territories are established in a timely manner and then outline a possible sequence of the transcriptional activation events required during induction of the neural crest.

The ectodermal tissue can be divided into three principal domains (neural, neural fold and epidermis) in order to better explain the expression territories and action of the different transcription factors determining neural crest development. The two principal molecules involved in establishing gradients with positional information in the ectoderm at the early gastrula stage are BMP and Shh. In response to these signals, the expression of transcription factors is activated in each ectodermal domain or in superimposed domains. A broader discussion of transcriptional activity in the neural and epidermal domains exceeds the scope of this review, so we will only consider some aspects that influence neural crest formation. In the neural domain, pre-pattern genes of Gli and Zic families of transcription factors are successively activated in response to Shh and low levels of BMP (for a review on hedgehog/Gli signaling; see Ruiz Altaba , 1999, Brewster et al., 1998; Ruiz iAltaba et al., 2002). The combination of Gli and Zic genes activates pro-neural genes in the neural domain, which later on, once activated, are able to repress neural fold genes. The expression of Zic genes in the neural fold region may be controlled by the Meis1/Pbx1 genes, as over-expression of XMeis is able to upregulate Zic3 and Gli3 expression (Maeda et al., 2001; 2002). At the end of gastrulation, Zic1-3 disappears from the neural plate (Nakata et al., 1997; 1998, Mizuseki et al., 1998) and residual expression of Zic3 remains at the neural fold and is probably required to induce Zic5, which is expressed in a territory more restricted to the neural fold than other members of the Zic family. Over-expression of Zic5 induces neural crest genes in the absence of anterior neural markers (Nakata et al., 2000). In this sense, Zic5 seems to be downstream of other Zic genes but is likely to control more specifically the early specification of neural crest cells.

At the epidermal border of the neural folds, a series of ventral or epidermal genes are expressed, such as Msx, Dlx, Xvent, Gata, etc. Many of these genes are expressed as a result of elevated levels of BMP, and some of them are assumed to be direct targets of this secreted molecule. Recent studies have pointed to Msx-1 as a major factor in the initial specification of epidermis (Bendall and Abate-Shen, 2000; Maeda et al., 1997; Yamamoto et al., 2000; Takeda et al., 2000). Dlx3, Dlx5 and Dlx6 transcription factors are required for the development of the epidermis, and act as repressors of neural crest genes defining the lateral boundary for the cranial neural crest (Luo et al., 2001a, 2001b). By the end of gastrulation, the transcription factor AP-2 is restricted to prospective epidermis where it controls epidermal development by regulating the transcription of cytokeratin genes (Luo et al., 2002). AP-2 is later expressed in the pre-migratory neural crest but its function in this tissue remains unknown. The dynamic expression patterns of Msx1 and AP-2 genes suggest they could have dual functions, early in epidermal induction and later also in the neural crest induction. Thus, a cascade of different genes is activated at both sides of the neural fold, in the neural plate and the epidermis, which regulates the expression of neural crest genes.

On the other hand, in the neural fold domain a cascade of genes is activated, which positively controls the development of the neural crest. One of the earlier genes expressed in this cascade is Pax-3, although its transient expression at the border of the neural plate is not exclusive of the neural fold (Bang et al., 1997; 1999). When over-expressed, Pax-3 is able to up-regulate Xslug expression (Mayor et al. 2000). The best-characterized transcription factors expressed in the neural fold of Xenopus are the zinc finger proteins Xsnail and Xslug (Sargent and Bennett, 1990; Mayor et al., 1995), two representative members of the Snail superfamily (for review see Nieto, 2002). The earliest gene expressed specifically in the prospective neural crest region is Xsnail (Essex et al., 1993, Mayor et al., 1993; Linker et al. 2000; Aybar and Mayor, 2001; Aybar et al., 2002). This gene controls the transcription of Xslug and other transcription factors expressed in the neural crest such as Xtwist, FoxD3, Zic-5, Ets-1 (Linker et al., 2000; Mayor et al., 2000; Aybar et al., 2002). Xsnail upregulates these genes in the absence of expression of neural or mesodermal markers, strongly suggesting that Xsnail alone is sufficient to convert presumptive ectodermal cells into neural crest cells. Thus, Xsnail is likely to be one of the upstream gene groups in the genetic cascade controlling transcription at the neural fold. A new Xslug gene (Xslugß) has been identified recently in Xenopus (Vallin et al., 2001). Although both Xslug genes seem to yield almost identical protein products, they have very different 5´-flanking regions. The biological significance in neural crest development of these two very similar genes controlled by different promoters remains unknown. However, it has been demonstrated that induction of Xslug requires intermediate BMP levels and posteriorizing signals such as FGF, Wnt and Retinoic Acid (Villanueva et al., 2002). This indicates that the neural fold tissue could integrate different signaling pathways to define appropriate expression of tissue markers. Experiments employing over-expression, antisense oligonucleotides or dominant negatives of Xsnail and Xslug showed that both genes play a key role in neural crest development in different animal models (Mayor et al., 2000; LaBonne and Bronner-Fraser, 1998; LaBonne and Bronner-Fraser, 2000; Carl et al., 1999; Nieto et al., 1994; Del Barrio and Nieto, 2002; Aybar et al., 2002; Tribulo and Mayor, 2001).

FoxD3, a member of the Forkhead box- (Fox) or winged-helix class of transcription factors, was also found to play a role in neural crest specification. This gene is expressed in premigratory and migratory neural crest of Xenopus, chick and zebrafish. Over-expression of FoxD3 in Xenopus induces different neural crest markers and that expression of FoxD3 also ocurrs in response to BMP and Wnt signals (Sasai et al., 2001). Studies in chicken showed that FoxD3 is not able to induce Slug or RhoB (Dottori et al., 2001) but rather participates in establishing neural crest lineages by repressing melanogenesis (Kos et al., 2001,).

The basic Helix-Loop-Helix (bHLH) class transcription factor Twist is also expressed in the pre-migratory and migratory neural crest cells but very little is known about its function in the neural crest (Hopwood et al., 1989; Linker et al., 2000). SRY-like HMG box (Sox)-containing genes, such as Sox9 of zebrafish (Li et al., 2002) and Xenopus (Spokony et al., 2002), are expressed in the neural fold. In Xenopus, Sox9 expression persists as development proceeds in migrating cranial crest cells that populate the pharyngeal arches. However, in zebrafish, neural crest cells cease to express Sox9b expression during migration (Li et al., 2002). Depletion of Sox9 protein in developing Xenopus embryos, using morpholino antisense oligos, causes a dramatic loss of neural crest progenitors and an expansion of the neural plate (Spokony et al., 2002). The transcription factor Sox10 is required for proper development of various neural crest-derived cell types. Melanocytes, autonomic and enteric neurons, and all subtypes of peripheral glia are missing in mice homozygous for Sox10 mutations and in zebrafish with the same Sox10 mutations (Dutton et al., 2001a; 2001b; Kelsh and Eisen, 2000; Kelsh et al., 2000). The available experimental evidence indicates that Sox10 is required in neural crest stem cells prior to lineage segregation (Paratore et al., 2001).

One other transcription factor expressed in the neural crest of zebrafish, chicken and Xenopus is the bHLH factor Id3 (Dickmeis et al., 2002; Reynaud-Deonauth et al., 2002; Kee and Bronner-Fraser, 2001a). The presence of Su(H) binding sites in both the enhancer and the promoter of XId3, suggest that Notch-dependent control of differentiation may involve activation of transcription of Id genes (Reynaud-Deonauth et al., 2002). In chick the Id4 gene is expressed in subsets of migrating neural crest cells (Kee and Bronner-Fraser, 2001b); however the function of this and other members of Id gene family in the neural crest is not yet understood.

Lecture overview

In this lecture, results from our laboratory concerning development of the neural crest will be discussed. A new model for induction of the neural crest that involves a gradient of BMP in combination with posteriorizing signals will be presented (Marchant et al., 1998; Mayor and Aybar, 2001; Villanueva et al., 2002; Aybar and Mayor, 2002). This model is based on experiments analyzing the expression of specific markers for the anterior neural fold (cpl1, Xag) or the neural crest (Slug, Twist) under different conditions. In addition, gain- and loss- of function experiments were preformed to determine the role of FGF, BMPs and Retinoic Acid. The contribution of the wnt pathway and the role of Notch/Delta signaling were explored using different mutants of these molecules or their respective targets. Finally, we will discuss how transcription factors are activated in crest cells. In particular, we will concentrate on the role the two zinc finger genes Snail and Slug play during early specification and migration of crest cells.


Work in RM laboratory is currently supported by an International Research Scholar Award from the Howard Hughes Medical Institute to RM and by grants from Fondecyt (#1020688 to RM, #3010061 to MJA), Fundación Antorchas (#13953-3 to MJA) and the Millennium Program (P99-137F to RM).


AYBAR MJ, MAYOR R (2001) Participación del gen Xsnail en el desarrollo de la cresta neural de Xenopus laevis. Biol Res 34:R44         [ Links ]

AYBAR MJ, MAYOR R (2002) Early induction of neural crest cells: lessons learned from frog, fish and chick. Curr Opin Genet Dev 12:452-548         [ Links ]

AYBAR MJ, NIETO MA, MAYOR R (2002) Snail precedes Slug in the genetic cascade required for the specification and migration of the Xenopus neural crest. Submitted         [ Links ]

BAKER NE (2000) Notch signaling in the nervous system. Pieces still missing from the puzzle. Bioessays 22:264-273         [ Links ]

BANG AG, PAPALOPULU N, KINTNER C, GOULDING MD (1997) Expression of Pax-3 is initiated in the early neural plate by posteriorizing signals produced by the organizer and by posterior non-axial mesoderm. Development 124:2075-2085         [ Links ]

BANG AG, PAPALOPULU N, GOULDING MD, KINTNER C (1999) Expression of Pax-3 in the lateral neural plate is dependent on a Wnt-mediated signal from posterior nonaxial mesoderm. Dev Biol 212:366-380         [ Links ]

BARTH KA, KISHIMOTO Y, ROHR KB, SEYDLER C, SCHULTE-MERKER S, WILSON SW (1999) BMP activity establishes a gradient of positional information throughout the entire neural plate. Development 126:4977-4987         [ Links ]

BENDALL AJ, ABATE-SHEN C (2000) Roles for Msx and Dlx homeoproteins in vertebrate development. Gene 247:17-31         [ Links ]

BOUWMEESTER T, KIM S, SASAI Y, LU B, DE ROBERTIS EM (1996) Cerberus is a head-inducing secreted factor expressed in the anterior endoderm of Spemann's organizer. Nature 382:595-601         [ Links ]

BREWSTER R, LEE J, RUIZ I ALTABA A (1998) Gli/Zic factors pattern the neural plate by defining domains of cell differentiation. Nature 393:579-583         [ Links ]

CARL TF, DUFTON C, HANKEN J, KLYMKOWSKY MW (1999) Inhibition of neural crest migration in Xenopus using antisense slug RNA. Dev Biol 213:101-115         [ Links ]

CHANG C, HEMMATI-BRINVALOU A (1998) Neural crest induction by Xwnt7B in Xenopus. Dev Biol 1998 194:129-134         [ Links ]

CHRIST B, HUANG R, WILTING J (2000) the development of the avian vertebral column. Anat Embriol 202(3):179-194         [ Links ]

COFFMANN C, WILIAM H, KINTNER C (1990) Xotch, the Xenopus homolog of Drosophila Notch. Science 249:1438-1441         [ Links ]

CORNELL RA, EISEN JS (2002) Delta/Notch signaling promotes formation of zebrafish neural crest by repressing Neurogenin 1 function. Development 129:2639-2648         [ Links ]

DEARDORFF MA, TAN C, SAINT-JEANNET JP, KLEIN P (2001) A role for frizzled 3 in neural crest development. Development 128:3655-3663         [ Links ]

DEL BARRIO MG, NIETO MA (2002) Overexpression of Snail family members highlights their ability to promote chick neural crest formation.Development 2002 129:1583-1593         [ Links ]

DICKMEIS T, RASTEGAR S, LAM CS, AANSTAD P, CLARK M, FISCHER N, ROSA F, KORZH V, STRAHLE U (2002) Expression of the helix-loop-helix gene id3 in the zebrafish embryo. Mech Dev 113:99-102         [ Links ]

DORSKY RI, MOON RT, RAIBLE DW (1998) Control of neural crest cell fate by the Wnt signalling pathway. Nature 396:370-373         [ Links ]

DOTTORI M, GROSS MK, LABOSKY P, GOULDING M (2001) The winged-helix transcription factor Foxd3 suppresses interneuron differentiation and promotes neural crest cell fate. Development 128:4127-4138         [ Links ]

DUTTON KA, PAULINY A, LOPEZ SS, ELWORTHY S, CARNEY TJ, RAUCH J, GEISLER R, HAFFTER P, KELSH RN (2001a) Zebrafish colourless encodes Sox10 and specifies non-ectomesenchymal neural crest fates. Development 128:4113-4125         [ Links ]

DUTTON K, DUTTON JR, PAULINY A, .KELSH RN (2001b) A morpholino phenocopy of the colourless mutant. Genesis 30:188-189         [ Links ]

ENDO Y, OSUMI N, WAKAMATSU Y (2002) Bimodal functions of Notch-mediated signaling are involved in neural crest formation during avian ectoderm development. Development 129:863-873         [ Links ]

ERTER CE, WILM TP, BASLER N, WRIGHT CV, SOLNICA-KREZEL L (2001) Wnt8 is required in lateral mesendodermal precursors for neural posteriorization in vivo. Development 128:3571-3583         [ Links ]

ESSEX LJ, MAYOR R, SARGENT MG (1993) Expression of Xenopus Snail in mesoderm and prospective neural fold ectoderm. Dev Dyn 198:108-122         [ Links ]

HASHIMOTO H, ITOH M, YAMANAKA Y, YAMASHITA S, SHIMIZU T, SOLNICA-KREZEL L, HIBI M, HIRANO T (2000) Zebrafish Dkk1 functions in forebrain specification and axial mesendoderm formation. Dev Biol 217:138-152         [ Links ]

HENDRIKS, B AND REICHMANN E (2002) Wnt signaling: a complex isuue. Biol Res 35: 277-286         [ Links ]

HOPWOOD ND, PLUCK A, GURDON JB (1989) A Xenopus mRNA related to Drosophila twist is expressed in response to induction in the mesoderm and the neural crest. Cell 59:893-903         [ Links ]

IKEYA M, LEE SM, JOHNSON JE, MCMAHON AP, TAKADA S (1997) Wnt signalling required for expansion of neural crest and CNS progenitors. Nature 389:966-970         [ Links ]

JENNINGS B, PREISS A, DELIDAKIS C, BRAY S (1994) The Notch signaling pathway is required for Enhancer of Split bHLH protein expression during neurogenesis in the Drosophila embryo. Development 120:3537-3548         [ Links ]

JING EJ, ERICKSON CA, TAKADA S, BURRUS LW (2001) Wnt and Bmp signaling govern lineage segregation of melanocytes in the avian embryo. Dev Biol 233:22-37         [ Links ]

KAZANSKAYA O, GLINKA A, NIEHRS C (2000) The role of Xenopus dickkopf1 in prechordal plate spercification and neural patterning. Development 127:4981-4992         [ Links ]

KEE Y, BRONNER-FRASER M (2001a) The transcriptional regulator Id3 is expressed in cranial sensory placodes during early avian embryonic development. Mech Dev 109:337-340         [ Links ]

KEE Y, BRONNER-FRASER M (2001b) Id4 expression and its relationship to other Id genes during avian embryonic development. Mech Dev 109:341-345         [ Links ]

KELSH RN, EISEN JS (2000) The zebrafish colourless gene regulates development of non-ectomesenchymal neural crest derivatives. Development 127:515-525.         [ Links ]

KELSH RN, SCHMID B, EISEN JS (2000) Genetic analysis of melanophore development in zebrafish embryos. Dev Biol. 225:277-293         [ Links ]

KIECKER C, NIEHRS C (2001) A morphogen gradient of Wnt/beta-catenin signalling regulates anteroposterior neural patterning in Xenopus. Development 128:4189-4201         [ Links ]

KIYOTA T, JONO H, KURIYAMA S, HASEGAWA K, MIYATANI S, KINOSHITA T (2001) X-Serrate-1 is involved in primary neurogenesis in Xenopus laevis in a complementary manner with X-Delta-1. Dev Genes Evol 211(8-9):367-376         [ Links ]

KOS R, REEDY MV, JOHNSON RL, ERICKSON CA (2001) The winged-helix transcription factor FoxD3 is important for establishing the neural crest lineage and repressing melanogenesis in avian embryos. Development 128:1467-1479         [ Links ]

LABONNE C, BRONNER-FRASER M (1998) Neural crest induction in Xenopus: evidence for a two-signal model. Development 1998 125:2403-2414         [ Links ]

LABONNE C, BRONNER-FRASER M (2000) Snail-related transcriptional repressors are required in Xenopus for both the induction of the neural crest and its subsequent migration. Dev Biol 221:195-205         [ Links ]

LE DOUARIN NM, KALCHEIM C (1999) The neural crest. Cambridge, UK: Cambridge University Press         [ Links ]

LEKVEN AC, THORPE CJ, WAXMAN JS, MOON RT (2001) Zebrafish Wnt8 encodes two Wnt8 proteins on a bicistronic transcript and is required for mesoderm and neurectoderm patterning. Dev Cell 1:103-114         [ Links ]

LI M, ZHAO C, WANG Y, ZHAO Z, MENG A (2002) Zebrafish Sox9b is an early neural crest marker. Genes Dev Evol 212:203-206         [ Links ]

LIEM KF, TREMML G, ROELINK H, JESSELL TM (1995) Dorsal differentiation of neural plate cells induced by BMP-mediated signals from epidermal ectoderm. Cell 82:969-979         [ Links ]

LINKER C, BRONNER-FRASER M, MAYOR R (2000) Relationship between gene expression domains of Xsnail, Xslug, and Xtwist and cell movement in the prospective neural crest of Xenopus. Dev Biol 2000 224:215-225         [ Links ]

LUO T, MATSUO-TAKASAKI M, LIM JH, SARGENT TD (2001a) Differential regulation of Dlx gene expression by a BMP morphogenetic gradient. Int J Dev Biol 45:681-684         [ Links ]

LUO T, MATSUO-TAKASAKI M, SARGENT TD (2001b) Distinct roles for Distal-less genes Dlx3 and Dlx5 in regulating ectodermal development in Xenopus. Mol Reprod Dev 60:331-337         [ Links ]

LUO T, MATSUO-TAKASAKI M, THOMAS ML, WEEKS DL, SARGENT TD (2002) Transcription factor AP-2 is an essential and direct regulator of epidermal development in Xenopus. Dev Biol 245:136-144         [ Links ]

MAEDA R, KOBAYASHI A, SEKINE R, LIN JJ, KUNG H, MAENO M (1997) Xmsx-1 modifies mesodermal tissue pattern along dorsoventral axis in Xenopus laevis embryo. Development 124:2553-2560         [ Links ]

MAEDA R, MOOD K, JONES TL, ARUGA J, BUCHBERG AM, DAAR IO (2001) Xmeis1, a protooncogene involved in specifying neural crest cell fate in Xenopus embryos. Oncogene. 20:1329-1342         [ Links ]

MAEDA R, ISHIMURA A, MOOD K, PARK EK, BUCHBERG AM, DAAR IO (2002) Xpbx1b and Xmeis1b play a collaborative role in hindbrain and neural crest gene expression in Xenopus embryos. Proc Natl Acad Sci USA 99:5448-5453         [ Links ]

MAYOR R, AYBAR M (2001) Induction and development of neural crest in Xenopus laevis. Cell & Tissue Research 305:203-209         [ Links ]

MAYOR R, VILLANUEVA S, GLAVIC A, BASTIDAS F, TRIBULO C (2001) Gradientes de moléculas involucradas en la inducción de las crestas neurales en Xenopus. Biol Res 34:R23         [ Links ]

MARCHANT L, LINKER C, RUIZ P, GUERRERO N, MAYOR R (1998) The inductive properties of mesoderm suggest that the neural crest cells are specified by a BMP gradient. Dev Biol 198:319-329         [ Links ]

MAYOR R, ESSEX LJ, BENNET MF, SARGENT MG (1993) Distinct elements of the Xsna promoter are requiered for mesodermal and ectodermal expression. Development 119:661-671         [ Links ]

MAYOR R, MORGAN R, SARGENT MG (1995) Induction of the prospective neural crest of Xenopus. Development 121:767-777         [ Links ]

MAYOR R, GUERRERO N, YOUNG RM, GOMEZ-SKARMETA JL, CUELLAR C (2000) A novel function for the Xslug gene: control of dorsal mesendoderm development by repressing BMP-4. Mech Dev 97:47-56         [ Links ]

MIZUSEKI K, KISHI M, MATSUI M, NAKANISHI S, SASAI Y (1998) Xenopus Zic-related-1 and Sox-2, two factors induced by chordin, have distinct activities in the initiation of neural induction. Development 125:579-587         [ Links ]

MORGAN R, SARGENT MG (1997) The role in neural patterning of translation initiation factor eIF4AII; induction of neural fold genes. Development 124:2751-2760         [ Links ]

MORRISON S, PEREZ S, QIAO Z, VERDI J, HICKS C, WEINMASTER G, ANDERSON D (2000) Transient Notch activation initiates an irreversible switch from neurogenesis to gliogenesis by neural crest stem cells. Cell 101:499-510         [ Links ]

NAKATA K, NAGAI T, ARUGA J, MIKOSHIBA K (1997) Xenopus Zic3, a primary regulator both in neural and neural crest development. Proc Natl Acad Sci USA 94:11980-11985         [ Links ]

NAKATA K, NAGAI T, ARUGA J, MIKOSHIBA K (1998) Xenopus Zic family and its role in neural and neural crest development. Mech Dev 75:43-51         [ Links ]

NAKATA K, KOYABU Y, ARUGA J, MIKOSHIBA K (2000) A novel member of the Xenopus Zic family, Zic5, mediates neural crest development. Mech Dev 99:83-91         [ Links ]

NELLEMANN C, BELLARD ME, BAREMBAUM M, LAUFER E, BRONNER-FRASER M (2001) Excess Lunatic Fringe causes cranial neural crest over-proliferation. Dev Biol 235:121-130         [ Links ]

NGUYEN VH, SCHMID B, TROUT J, CONNORS SA, EKKER M, MULLINS MC (1998) Ventral and lateral regions of the zebrafish gastrula, including the neural crest progenitors, are stablished by a bmp2/swirl pathway of genes. Dev Biol 199:103-110         [ Links ]

NIETO MA (2002) The Snail superfamily of zinc-finger transcription factors. Nat Rev Mol Cell Biol 3:155-166         [ Links ]

NIETO MA, SARGENT MG, WILKINSON DG, COOKE J (1994) Control of cell behavior during vertebrate development by Slug, a zinc finger gene. Science 264:835-839         [ Links ]

OGITA J, ISOGAI E, SUDO H, SAKIYAMA S, NAKAGAWARA A, KOSEKI H (2001) Expression of the Dan gene during chicken embryonic development. Mech Dev 109:363-365         [ Links ]

PARATORE C, GOERICH DE, SUTER U, WEGNER M, SOMMER L (2001) Survival and glial fate acquisition of neural crest cells are regulated by an interplay between the transcription factor Sox10 and extrinsic combinatorial signaling. Development 128, 3949-3961         [ Links ]

REYNAUD-DEONAUTH S, ZHANG H, AFOUDA A, TAILLEFERT S, BEATUS P, KLOC M, ETKIN LD, FISCHER-LOUGHEED J, SPOHR G (2002) Notch signaling is involved in the regulation of Id3 gene transcription during Xenopus embryogenesis. Differentiation 69:198-208         [ Links ]

RUIZ I ALTABA A (1999) Gli proteins and Hedgehog signaling: development and cancer. Trends Genet 15:418-425         [ Links ]

RUIZ I ALTABA A, PALMA V, DAHMANE N (2002) Hedgehog-Gli signalling and the growth of the brain. Nat Rev Neurosci 3:24-33         [ Links ]

SAINT-JEANNET JP, HE X, VARMUS HE, DAWID IB (1997) Regulation of the dorsal fate in the neuraxis by Wnt-1 and Wnt-3a. Proc Natl Acad Sci USA 94:13713-13718         [ Links ]

SARGENT MG, BENNETT MF (1990) Identification in Xenopus of a structural homologue of the Drosophila gene snail. Development 109:967-973         [ Links ]

SASAI N, MIZUSEKI K, SASAI Y (2001) Requirement of FoxD3-class signaling for neural crest determination in Xenopus. Development 128:2525-2536         [ Links ]

SCHEER N, GROTH A, HANS S, CAMPOS-ORTEGA JA (2001) An instructive function for Notch in promoting gliogenesis in the zebrafish retina. Development 128:1099-1107         [ Links ]

SELLECK MAJ, GARCÍA-CASTRO MI, ARTINGER KB, BRONNER-FRASER M (1998) Effects of Shh and Noggin on neural crest formation demonstrate that BMP is required in the neural tube but not ectoderm. Development 125:4919-4930         [ Links ]

SILVA F, GLAVIC A, MAYOR R (2001) Análisis de la función Notch/Delta sobre el desarrollo de las crestas neurales de Xenopus laevis. Biol Res 34: R75         [ Links ]

SPOKONY RF, AOKI Y, SAINT-GERMAIN N, MAGNER-FINK E, SAINT-JEANNET JP (2002) The transcription factor Sox9 is required for cranial neural crest development in Xenopus. Development 129:421-432         [ Links ]

STRUHL G, ADACHI A (2000) Requirement for Presenilin-dependent cleavage of Notch and other transmembrane proteins. Mol Cell 6:625-636         [ Links ]

STRUHL G, GREENWALD I (2001) Presenilin-mediated transmembrane cleavage is required for Notch signal transduction in Drosophila. Proc Natl Acad Sci USA 98:229-235         [ Links ]

TAKEDA M, SAITO Y, SEKINE R, ONITSUKA I, MAEDA R, MAENO M (2000) Xenopus msx-1 regulates dorso-ventral axis formation by suppressing the expression of organizer genes. Comp Biochem Physiol B Biochem Mol Biol 2000 126:157-168         [ Links ]

TAN C, DEARDORFF MA, SAINT-JEANNET JP, YANG J, ARZOUMANIAN A, KLEIN P (2001) Kermit, a frizzled interacting protein, regulates frizzled 3 signalling in neural crest development. Development 128: 3665-3674         [ Links ]

TRIBULO C, MAYOR R (2001) Análisis de la apoptosis durante el desarrollo de la cresta neural en Xenopus laevis. Biol Res 34: R76         [ Links ]

VALLIN J, THURET R, GIACOMELLO E, FARALDO MM, THIERY JP, BRODERS F (2001) Cloning and characterization of three Xenopus Slug promoters reveal direct regulation by Lef/ß-catenin signaling. J Biol Chem 276:30350-30358         [ Links ]

VILLANUEVA S, GLAVIC A, RUIZ P, MAYOR R (2002) Posteriorization by FGF, Wnt, and Retinoic Acid is required for neural crest induction. Dev Biol 2002 241:289-301         [ Links ]

WILSON PA, LAGNA G, SIZUKI A, HEMMATI-BRINVALOU A (1997) Concentration-dependent patterning of the Xenopus ectoderm by BMP4 and its signal transducer Smad1. Development 124: 3177-3184         [ Links ]

WILSON SI, RYDSTRÖM A, TRIMBORN T, WILLERT K, NUSSE R, JESSELL TM, EDLUND T (2001) The status of Wnt signaling regulates neural and epidermal fates in the chick embryo. Nature 411:325-330         [ Links ]

YAMAMOTO TS, TAKAGI C, UENO N (2000) Requirement of Xmsx-1 in the BMP-triggered ventralization of Xenopus embryos. Mech Dev 91:131-141         [ Links ]

Corresponding author: Roberto Mayor. tel: 56 2 6787351. fax: 562 271 2983. e-mail:

Received: May 30, 2002. In revised form: June 13, 2002. Accepted: July 11, 2002

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License