SciELO - Scientific Electronic Library Online

vol.37 número4CALCIUM SIGNALING: A UNIVERSAL MECHANISM OF CELLULAR COMMUNICATIONFunctional implications of RyR-DHPR relationships in skeletal and cardiac muscles índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados




Links relacionados


Biological Research

versión impresa ISSN 0716-9760

Biol. Res. v.37 n.4 Santiago  2004 


Biol Res 37: 497-505, 2004


Calcium signaling: A historical account


Department of Biochemistry, University of Padova, and Venetian Institute of Molecular Medicine (VIMM), 35121 Padova, Italy

Key words: Calcium signaling, calcium sensors, calcium transporters.

Dirección para Correspondencia


The idea that Ca2+ could be a carrier of signals originated over 100 years ago with the observation by S. Ringer that isolated hearts could only be made to contract if Ca2+ was added to the perfusion medium (50). The demonstration that the effect of Ca2+ was intracellular and cation specific only came after a long interval of time: it emerged from work performed in 1947 by Heilbrunn and Wiercinski who elicited the contraction of frog muscles by injecting Ca2+ into them, whereas no contraction followed the injection of Na+, K+ or Mg2+ (29). While these results unequivocally linked intracellular Ca2+ to muscle contraction, the extension of the concept of Ca2+ signaling to processes other than muscle contraction was slow in coming. In 1973 Miledi induced neurotransmitter release by injecting Ca2+ into the pre-synaptic terminal of squid axons (42), and others elicited responses by injecting Ca2+ into mastocytes (33), salivary gland cells (49), or oocytes (61). In parallel with these studies, several reports linked the control of the Ca2+ signals to the reversible complexation of the cation by specific proteins, showing that cells contained soluble proteins that buffered Ca2+ and, especially, membrane-linked proteins that rapidly and efficiently shifted its concentration within cells by transporting it across membranes. The membrane-intrinsic Ca2+-transporting systems in the plasma membrane, mitochondria, endoplasmic reticulum, the Golgi system, and the nucleus, will all be briefly described here, but a succinct discussion of the soluble (or non-membrane intrinsic) Ca2+-binding proteins which process Ca2+ information will precede the description.


The most important proteins able to bind Ca2+ with the affinity and specificity required for the regulation of its concentration in the intracellular environment belong to the EF-hand family (34), which now contains hundreds of members; some only regulate only one Ca2+-dependent process (enzyme), e.g., recoverin, troponin C, while others are not target-specific, e.g., calmodulin, which is the most thoroughly studied EF-hand protein. The structural principles underlying the Ca2+-binding function of calmodulin (which corresponds to that of all other proteins of the family) had been originally extrapolated from the crystal structure of parvalbumin (35) but were validated directly by the solution of its three dimensional structure (3). In the structure of EF-hand proteins, domains made of two orthogonal a-helices are interrupted by a 10-12 amino acid loop that coordinates Ca2+ to oxygens of some invariant residues. This helix-loop-helix Ca2+ binding motif, which is normally repeated several times in the molecules, has been optimized in the course of evolution and it is now found in hundreds of proteins. However, the Ca2+ buffering function of EF-hand proteins is secondary to their ability to process the Ca2+ signal. The decoding process is performed by two sequential conformational changes (31, 40). The first exposes hydrophobic domains on the surface of the protein upon binding Ca2+, enabling it to interact with targets. The second collapses the elongated structure of the protein (calmodulin) around its binding domain in the target enzyme, transmitting to it the Ca2+ information.

A quantitative argument must be considered at this point: the total amount of EF-hand proteins (and of other types of proteins that may process the Ca2+ signal, e.g., annexins or gelsolin) is finite and could become quantitatively inadequate in cases of large swings in cellular Ca2+ concentration. These quantitative restrictions do not apply to membrane-intrinsic Ca2+ binding (and transport) proteins, which complex Ca2+ at one membrane side, transport it across, and discharge it to the other side, continuously repeating the operation. These proteins thus play the most important role in the buffering of intracellular Ca2+, satisfying both the demands for rapid and high-affinity regulation and for lower affinity regulation. For high-affinity Ca2+ regulation, cells depend on ATP-driven pumps; for lower affinity regulation, they have more options.


1. The plasma membrane

The plasma membrane possesses several types of channels which control the downhill diffusion of Ca2+ into cells, and two systems that extrude it: a high-affinity, low-capacity pump, and a lower affinity, large-capacity Na+/Ca2+ exchanger (Figure 1). The plasma membrane is evidently responsible for the 10,000-fold gradient of Ca2+ normally measured between the extracellular space and the cytoplasm. However, cells exchange across the plasma membrane only a minor portion of the total Ca2+ they need for their activities, using instead for most of their signaling requirements Ca2+ stored in the intracellular organelles. Importantly, however, the limited amount of Ca2+ penetrating through the plasma membrane triggers cascades of events that are vital to cell activity. Another point on the traffic of Ca2+ across the plasma membrane deserves to be mentioned: the very large gradient of Ca2+ across the plasma membrane not only ensures unlimited availability of the cation. It is dynamically beneficial, since in its presence even minor changes in the Ca2+ permeability of the plasma membrane would ensure significant swings in the intracellular Ca2+ concentration. Unfortunately, the large gradient also creates a potential situation of danger. If the Ca2+ permeability barrier of the plasma membrane were to fail, which is a frequent event in pathology, the cell would be inundated by Ca2+ and its reversible messenger function would come to an end.

Many types of Ca2+ channels operate in the plasma membrane: some are gated by ligands (e.g., the channels activated by glutamate in post-synaptic membranes) or by the emptying of the cytoplasmic Ca2+ stores (48), but the most important are the voltage-gated channels, which are typical of excitable tissues, e.g. heart. Of the two plasma membrane Ca2+-exporting systems, the Na+/Ca2+ exchanger has low Ca2+ affinity but high Ca2+ transporting velocity and is particularly active in excitable cells, e.g., heart (see reference 46 for a recent review). The exchanger operates electrogenically, exchanging 3 Na+ for 1 Ca2+, and thus responds both to the gradients of Ca2+ and Na+ across the plasma membrane and to the transmembrane potential.

Three basic exchanger gene products are known: NCX1, NCX2, and NCX3. The first two are ubiquitously distributed in tissues, while NCX3 is restricted to brain. They are organized in the membrane with nine transmembrane domains and a large cytosolic loop separating transmembrane domains 5 and 6. The large loop contains regulatory sites, e.g., a putative calmodulin binding domain and an allosteric Ca2+-binding site. A particular variant of the exchanger, which is active in retinal photoreceptors, exchanges Ca2+ for Na+ and K+.

Figure 1. The control of cellular Ca2+
Three types of channels gated by ligands, by the emptying of cellular Ca2+ stores, and by voltage mediate Ca2+ entry into cells. The intracellular Ca2+ pool is regulated by binding to Ca2+-binding proteins (e.g., calmodulin) and by Ca2+ transport across the plasma membrane and the membranes of organelles. In addition to buffering Ca2+, soluble Ca2+-binding proteins (e.g., calmodulin) also decode its message. Ca2+ enters the sarco(endo)plasmic reticulum through the sarco(endo)plasmic reticulum Ca2+ ATPase (the SERCA pump) and is released through channels that require effectors like inositol-(1,4,5)-trisphosphate (InsP3) or cyclic ADP ribose (cADPr). The Figure does not show the Ca2+-mobilizing messenger NAADP because the store on which it functions is still undefined. The release route from the sarco(endo)plasmic reticulum creates Ca2+ hotspots, which activate the low-affinity electrophoretic uniporter of neighboring mitochondria. Ca2+ exits mitochondria through a Na+/Ca2+ exchanger (a H+/Ca2+ release exchanger has also been described). Ca2+ can also be exchanged between the cytosol and the nucleus. The nuclear envelope is a genuine Ca2+ reservoir that shares transporters and channels with the ER (not shown in the Figure). The dashed arrows in and out of the nucleus highlight the present debate on Ca2+ traffic through the nuclear envelope (permanent passive permeability versus reversible gating of the pores). Ca2+ is expelled from the cell by a high-affinity/low-capacity plasma membrane Ca2+ ATPase (PMCA pump) and a low-affinity/high-capacity Na+/Ca2+ exchanger. The Golgi vesicles accumulate Ca2+ through a SERCA pump and also through an ATPase that differs from both the SERCA and PMCA pumps and release it through channels of which only that gated by InsP3 has so far been certified.


The other Ca2+-exporting system of the plasma membrane is the Ca2+ pump (PMCA), a member of the P-type ion motive ATPase family (see reference 26 for a recent review). The pump has high Ca2+ affinity (Km < 0.5 mM) when complexed with calmodulin. In the absence of calmodulin, it has a Ca2+ affinity comparable to that of the Na+/Ca2+ exchanger. The C-terminal calmodulin binding domain interacts with the cytoplasmic portion of the pump next to the active site and maintains it in a inhibited state until calmodulin removes it, re-establishing full activity of the pump. This inhibition/deinhibition mechanism is reminiscent of that operating in the SERCA pump, where the accessory protein phospholamban maintains the enzyme inhibited until it is removed from the interacting site on the pump by two kinase-mediated phosphorylations. The PMCA pump is organized in the membrane with 10 transmembrane domains, with most of its mass protruding into the cytoplasm.

In animals, the pump is the product of four genes, two (1 and 4) expressed ubiquitously in tissues, two (2 and 3) expressed only in brain and a few other cell types (58). In addition to the four basic gene products, a complex pattern of alternative spicing involving either a domain close to an N-terminal phospholipid interacting site or the C-terminal calmodulin binding domain increases the number of pump isoforms. Of interest are truncated versions resulting from the inclusion of an insert encoded by an extra exon in the C-terminal tail. These truncated isoforms are expected to have lower calmodulin affinity, since the insertion occurs in the middle of the calmodulin binding domain.

2. Intracellular organelles

In the last few years, intracellular organelles—such as the endoplasmic reticulum, the nucleus, the mitochondria, and more recently, the Golgi system—have emerged as crucial to the generation and transduction of Ca2+ signals (52). Ca2+ in the organelles is important not only in the control of the general Ca2+ homeostasis in cells but may also act as a specific regulator of the function of the organelles. A striking example is that of mitochondria, which contain three essential dehydrogenases that are exquisitely sensitive to Ca2+.

2.1 The endoplasmic reticulum

Historically, endoplasmic reticulum (or rather, its muscle cell counterpart, sarcoplasmic reticulum, see below) has played the most important role in the development of the concepts of organellar Ca2+ homeostasis. The landmark contributions by Hasselbach and Makinose, and Ebashi and Lipmann, in the 1960s showed ATP-dependent Ca2+ uptake in muscle microsomal preparations (19, 28). It is now recognized that the maintenance of Ca2+ homeostasis within the ER/SR is essential to a number of cell functions, ranging from cell growth, to protein synthesis and folding, to protein processing and transport. The endoplasmic reticulum (ER) and its tissue-specialized species, sarcoplasmic reticulum (SR), are the main dynamic Ca2+ storage compartment of eukaryotic cells. They contain a protein pump (SERCA-ATPase) for Ca2+ uptake, Ca2+ binding proteins for Ca2+ storage (calsequestrin and calreticulin), and channels for Ca2+ release (the inositol 1,4,5 trisphosphate [InsP3] receptor, InsP3R, and the ryanodine- receptor, RyR) (4, 5, 11).

The SERCA pump has 10 transmembrane domains, and its tri-dimensional structure has been recently solved at atomic resolution in both the Ca2+-bound and the Ca2+-free states (63, 65). The structure has permitted the identification of the location of all important domains, including the ATP binding site and the catalytic aspartyl residue, which are located in a large globular domain protruding between the fourth and fifth transmembrane sectors. All ten transmembrane domains and the cavity that forms the Ca2+ binding sites have also been located.

Among the SERCA pumps isoforms, only the cardiac type (which includes the smooth muscle and the slow-twitch muscle isoforms) is regulated by the small hydrophobic protein phospholamban (37) briefly mentioned above. Dephosphorylated phospholamban binds to the SERCA pump, shifting its Kd for Ca2+ to a lower affinity value and thus inhibiting the pump activity. Phosphorylation of phospholamban by the cAMP and Ca2+/calmodulin-dependent protein kinases dissociates it from its binding site near the active site of the pump (2), relieving the inhibition.

While the InsP3 that will activate the InsP3-sensitive Ca2+-release channels (InsP3R) is generated by phospholipase C acting on phosphatidylinositol 4,5-bisphosphate at the plasma membrane in response to a number of first messengers, activation of Ca2+ release in ryanodine receptors (RyR) in the terminal cisternal of sarcoplasmic reticulum is triggered directly by plasma membrane depolarization, through direct coupling (i.e., charge transfer) with L-type channels in the T-system in skeletal muscles. However, actual influx of external Ca2+ through the T-system L-type channels is instead responsible for the activation for the cardiac RyR. It might be mentioned at this point that, even if they are activated by InsP3, InsP3 receptors are also sensitive to Ca2+, which thus gates its release from all intracellular channels. Recently, the NAD+ metabolite-derivative cyclic ADP-ribose (cADPr), originally discovered as a potent Ca2+ mobilizing agent in sea urchin eggs, has been reported to stimulate Ca2+ release in higher eukaryotic systems as well. It is likely to act on the ryanodine receptors. A NADP metabolite, nicotinic acid adenine dinucleotide phosphate (NAADP) is the newest addition to the family of activators of intracellular Ca2+ channels (23). It discharges a Ca2+ pool located in a membrane compartment different from those sensitive to InsP3 and cADPr (see also Chini, this issue, reference 10).

The genes that encode the InsP3R and the RyR have now been cloned (21, 44, 60). The InsP3R/Ca2+ release channel is a complex of four subunits (7, 38, 41), organized in a square having a pinwheel appearance with fourfold symmetry (possibly, a channel pore). RyRs were purified from skeletal and cardiac muscles long ago and were also found to be multimeric complexes of very large subunits. Also in this case, electron microscopy and metal shadowing techniques have revealed a fourfold symmetric complex having the shape of a square prism with a bump projecting from the center of one face (32, 66). The number of transmembrane domains in the RyRs is controversial, but it is now accepted that each monomer of the tetrameric InsP3R contains six transmembrane domains and a large loop between transmembrane domains 5 and 6 which folds within the membrane to form the Ca2+ channel (see reference 20 for a comprehensive review).

Within the lumen of the ER, Ca2+ is buffered by Ca2+-binding proteins, which ensure the storage of large amounts of the cation. Several have been described, among them calsequestrin and calreticulin. These two proteins are not EF-hand proteins: clusters of acidic residues at their COOH-termini are responsible for Ca2+ binding.

2.2 The mitochondria

The uptake of Ca2+ by mitochondria was postulated by Chance in 1956 based on experiments on the stimulation of electron transport by Ca2+ (8), and was experimentally demonstrated by Vasington and Murphy in 1962 (64). Originally, Ca2+ handling by mitochondria was simply described in terms of active uptake and passive release (9). Experimental measurements of the internally-negative membrane potential predicted by the chemiosmotic theory (43) across the inner membrane showed that the electrical gradient was the driving force for Ca2+ accumulation, which would be mediated by an electrophoretic uniporter (56). It was then discovered that, in addition to the electrogenic uptake uniporter, mitochondria also possess an electroneutral antiporter that extrudes Ca2+ from the matrix in exchange for Na+ (6). Later on a Ca2+:H+ exchange reaction was also discovered.

Subsequent work on isolated mitochondria showed that the electrogenic uniporter had low Ca2+ affinity and only became activated to appreciable levels when cytosolic Ca2+ reached 0.5-1.0 mM (47). Since this concentration was out of the physiological range in resting cells, interest on mitochondria as cytosolic Ca2+ regulators gradually faded, shifting the emphasis to the regulation of intramitochondrial Ca2+, owing to the Ca2+ dependence of some matrix dehydrogenases, i.e., the NAD+-dependent isocitrate-dehydrogenase, the 2-oxoglutarate-dehydrogenase, and the phosphatase that activates pyruvate-dehydrogenase (13).

The extrapolation of the results on mitochondrial Ca2+ transport to the in vivo situation was prevented by the difficulty of measuring intramitochondrial Ca2+ in living cells. Eventually, the targeting of aequorin to mitochondria permitted the reliable measurement of Ca2+ in mitochondria in living cells. Mitochondrial calcium spikes occur in response to receptor agonists that promote Ca2+ release from endoplasmic reticulum or plasma membrane channel activation, which increase the cytoplasmic Ca2+ concentration in the vicinity of mitochondria to the micromolar range, i.e., the concentration required for the optimal activation of the uptake uniporter (51). As a result of these and other findings, interest in mitochondrial Ca2+ transport enjoyed a robust renaissance.

2.3 The nucleus

Although most of the traditional targets of Ca2+ signaling are located in the cytosol or at the plasma membrane (calmodulin, protein kinase C, ion channels, etc.) recent evidence has indicated that several nuclear processes (the breakdown of the nuclear envelope, transcriptional activation, DNA metabolism, etc.) are modulated by changes of Ca2+ in the nucleoplasm (12, 22, 62; also, see references 53 and 54 for recent comprehensive reviews). It has also been shown that nuclear Ca2+ may have roles that are distinct from those of cytosolic Ca2+, the effects being mediated by the translocation of transcription factors (18) and/or protein kinases (14) into the nucleus. Several reports have shown that nuclear Ca2+ activates a number of immediate early genes through the action of calmodulin-dependent kinases (CaMKIV) (25, 27).

The issue of the control of nuclear calcium traditionally has been controversial, mostly due to the existence of large pores in the envelope. The nuclear envelope has been viewed either as a structure that offers no barrier to the movements of calcium, which would thus freely and immediately equilibrate between the cytosolic and the nuclear compartments, or as an effective sieve that instead limits its traffic between the two compartments. However, irrespective of the presence and function of the pores, the nuclear envelope is able to transport Ca2+. A Ca2+-ATPase identical to the SERCA enzyme (36) and InsP3 and Ry (cADPr) receptors (39, 55), have now been documented in the envelope. Several groups have now shown that InsP3 and cADP-ribose may provoke Ca2+ release from the nuclear membrane into the nucleoplasm (24, 45, 55). These findings rationalize earlier work showing that the b-1-isoform of phospholipase C (PLC) is also localized to the inner side of the nuclear membrane, where it may mediate InsP3 generation (15, 16). As for cADPr, evidence also has been provided recently for the presence of its synthesizing enzyme in the nuclear envelope (1). An important problem which is still open in nuclear Ca2+ signaling is that of the transmission of the signals generated at the plasma membrane to the nuclear envelope to activate, for instance, the InsP3-producing machinery. Although contributions on this have begun to appear (17, 57), the field is still largely unexplored.

2.4 The Golgi

This organelle is the latest addition to the list of Ca2+ transporters. It contains a novel P-type ATPase (59) that has extremely high affinity for Ca2+ and which accumulates Ca2+ into the vesicles. The accumulated Ca2+ is released to the cytosol through channels of which so far only that sensitive to InsP3 has been documented.


The development of concepts in the area of cellular Ca2+ signaling has advanced for six or seven decades in isolated steps separated by long intervals. Originally confined to muscle research, Ca2+ as a carrier of information has expanded to other areas. Once the ball started rolling, it all moved at a very rapid pace until Ca2+ as a significant agent eventually permeated all fields of biology. Knowledge on Ca2+ signaling continues to grow exponentially, enlisting topics such as regulation of gene expression, numerous aspects of neuronal function, the quality control of protein trafficking between membranes, and processes linked to the suffering and eventual demise of cells.


The original work by the authors has been supported by grants from the Italian National Research Council (Targeted Project on Biotechnology), from the Armenise Foundation, from the Italian Ministry of Scientific Research (P.R.I.N. 1998, 2000, 2001, 2002), and from the Human Frontier Science Program (HFSP).


1. ADEBANJO OA, ANANDATHEERTHAVARADA HK, KOVAL AP, MOONGA BS, BISWAS G, SUN L, SODAM BR, BEVIS PJR, HUANG CLH, EPSTEIN S, LAI FA, AVADHANI NG, ZAIDI M (1999) A new function for CD38/ADP-rybosil cyclase in nuclear Ca2+ homeostasis. Nature Cell Biol 1: 409-414

2. AMES PH, PRUSCHY M, VORHERR T, PENNISTON JT, CARAFOLI E (1989) Primary structure of the cAMP-dependent phosphorylation site of the plasma membrane calcium pump. Biochemistry 28: 4253-4258

3. BABU YS, SACKS JS, GREEHOUGH TJ, BUGG CE, MEANS AR, COOK WJ (1985) Three-dimensional structure of calmodulin. Nature 315: 37-40

4. BERRIDGE MJ (1993) Inositol trisphosphate and calcium signalling. Nature 361: 315-325

5. BERRIDGE MJ (1997) Elementary and global aspects of calcium signalling. J Physiol (London) 499: 291-306

6. CARAFOLI E, TIOZZO R, LUGLI G, CROVETTI F, KRATZING C (1974) The release of calcium from heart mitochondria by sodium. J Mol Cell Cardiol 6: 361-371

7. CHADWICK CC, SAITO A, FLEISCHER S (1990) Isolation and characterization of the inositol trisphosphate receptor from smooth muscle. Proc Natl Acad Sci USA 87: 2132-2136

8. CHANCE B (1956) On possible mechanisms for the control of electron transport in the respiratory chain. In: LIEBECQC, C (ed) Proc 3rd Intern Congr Biochem. Brussels: Academic Press. pp: 300-304

9. CHANCE B (1965) The energy-linked reaction of calcium with mitochondria. J Biol Chem 240: 2729-2748

10. CHINI E (2004) To be or not to be...Nicotinic acid adenine dinucleotide phosphate (NAADP). A new intracellular second message? Biol Res 37: 559-563

11. CLAPHAM DE (1995) Calcium Signaling. Cell 80: 256-268

12. COLLART MA, TOURKINE N, BELIN D, VASSALLI P, JEANTEUR P, BLANCHARD JM (1991) c-fos gene transcription in murine macrophages is modulated by a calcium-dependent block to elongation in intron 1. Mol Cell Biol: 11 2826-2831

13. DENTON RM, MCCORMACK JG (1980) On the role of the calcium transport cycle in heard and other mammalian mitochondria. FEBS Lett 119: 1-8

14. DEWAARD M, STRUBE C, VILLAZ M (1998) Calcium channels and SNARE complex interacting for neurotransmitters exocytosis. MS Med Sci 14: 764-770

15. DIVECHA N, RHEES SG, LETCHER AJ, IRVINE RF (1993) Phosphoinositide signalling enzymes in rat liver nuclei: Phosphoinositidase C isoform beta 1 is specifically but not predominantly located in the nucleus. Biochem J 289: 617-620

16. DIVECHA N, BANFIC H, IRVINE RF (1994) The nuclear phosphoinositide cycle—does it play a role in nuclear Ca2+ homeostasis? Cell Calcium 16: 297-300

17. DIVECHA N, BANFIC H, IRVINE RR (1991) The polyphosphoinositide cycle exists in the nucleus of Swiss 3T3 cells under the control of a receptor (for IGF-1) in the plasma membrane and stimulation of cycle increases nuclear diacylglycerol and apparently induces translocation of protein kinase C to the nucleus. Embo J 10: 3207-3214

18. DOLMETSCH RE, LEWIS RS, GOODNOW CG, HEALY JI (1997) Differential activation of transcription factors induced by Ca2+ response amplitude and duration. Nature 386: 855-858

19. EBASHI S, LIPMANN F (1962) Adenosine-triphosphate-linked concentration of calcium ions in a particular fraction of rabbit muscle. J Cell Biol 14: 389-400

20. FURUICHI T, MICHIKAWA T, MIKOSHIBA K (1999) Intracellular calcium channels. In: CARAFOLI E, KLEE CB (eds) Calcium as a Cellular Regulator. Oxford: Oxford University Press. pp: 200-248

21. FURUICHI T, YOSHIKAWA S, MIKOSHIBA K (1989) Nucleotide sequence of cDNA encoding P400 protein in the mouse cerebellum. Nucleic Acids Res 17: 5385-5386

22. GAIDO ML, CIDLOWSKI JA (1991) Identification, purification and characterization of a calcium-dependent endonuclease (NUC18) from apoptotic rat thymocytes. J Biol Chem 266: 18580-18585

23. GENAZZANI AA, GALIONE A (1997) A Ca2+ release mechanism gated by the novel pyridine nucleotide NAADP. Trends Pharmacol Sci 18: 108-110

24. GERASIMENKO OV, GERASIMENKO JV, TEPIKIN AV, PETERSEN OH (1995) ATP-dependent accumulation and inositol trisphosphate—or cyclic ADP-ribose-mediated release of Ca2+ from the nuclear envelope. Cell 80: 439-444

25. GREENBERG ME, THOMPSON MA, SHENG M (1992) Calcium regulation of immediate early gene transcription. J Physiol Paris 86: 99-108

26. GUERINI D, CARAFOLI E (1999) The Calcium Pumps. In: CARAFOLI E, KLEE CB (eds) Calcium as a Cellular Regulator. Oxford: Oxford University Press. pp: 249-278

27. HARDINGHAM GE, CHAWLA S, JOHNSON CM, BADING H (1997) Distinct functions of nuclear and cytoplasmic calcium in the control of gene expression. Nature 385: 260-265

28. HASSELBACH W, MAKINOSE M (1961) Die calcium pumpe der "erschlaffungsgrana" des muskles und ihre abhangigkeitvon der ATP-spaltung. Biochem Z 333: 518-528

29. HEILBRUNN LV, WIERCINSKI FJ (1947) The action of various cations on muscle protoplasm. J Cell Comp Physiol 29: 15-32

30. HUMBERT JP, MATTER N, ARTAULT JC, KOPPLER P, MALVIYA AN (1996) Inositol 145-triphosphate receptor is located to the inner nuclear membrane vindicating regulation of nuclear calcium signaling by inositol 145-trisphosphate. Discrete distribution of inositol phosphate receptors to inner and outer nuclear membranes. J Biol Chem 271: 478-485

31. IKURA M, CLORE GM, GROENBORN AM, ZHU G, KLEE CB, BAX A (1992) Solution structure of a calmodulin-targeted peptide complex by multidimensional NMR. Science 256: 632-638

32. INUI M, SAITO A, FLEISCHER S (1987) Purification of the ryanodine receptor and identity with feet structure of junctional terminal cisternae of sarcoplasmic reticulum from fast skeletal muscle. J Biol Chem 262: 1740-1747

33. KANNO T, COCHRANE DE, DOUGLAS WW (1973) Exocytosis (secretory granule extrusion) induced by injection of calcium into mast cells. Can J Physiol Pharmacol 51: 1001-1004

34. KAWASAKI H, NAKAYAMA S, KRETSINGER RH (1998) Classification and evolution of EF-hand proteins. Biometals 11: 277-295

35. KRETSINGER RH, NOCKOLDS CE (1973) Carp muscle calcium-binding protein II: Structure, determination and general description. J Biol Chem 248: 3313-3326

36. LANINI L, BACH O, CARAFOLI E (1992) The calcium pump of the liver nuclear membrane is identical to that of endoplasmic reticulum. J Biol Chem 267: 11548-11552

37. LUO W, GRUPP IL, HARRER J et al (1994) Targeted ablation of the phospholamban gene is associated with markedly enhanced myocardial contractility and loss of beta-agonist stimulation. Circ Res 75: 401-409

38. MAEDA N, KAWASAKI T, NAKADE S, YOKOTA N, TANIGUCHI T, KASAI M, et al. (1991) Structural and functional characterization of inositol 1,4,5 trisphosphate receptor channel from mouse cerebellum. J Biol Chem 266: 1109-1116

39. MALVIYA AN, ROUGE P, VINCENDON G (1990) Stereospecific inositol 1,4,5-[32P]trisphosphate binding to isolated rat liver nuclei: Evidence for inositol trisphosphate receptor-mediated calcium release from the nucleus. Proc Natl Acad Sci USA 87: 9270-9274

40. MEADOR WE, MEANS AR, QUIOCHO FA (1992) Target enzyme recognition by calmodulin: 2.4 A structure of a calmodulin-peptide complex. Science 257: 1251-1255

41. MIGNERY GA, SUDHOF TC (1990) The ligand binding site and transduction mechanism in the inositol 1,4,5-trisphosphate receptor. Embo J 9: 3893-3898

42. MILEDI R (1973) Transmitter release induced by injection of calcium ions into nerve terminals. Proc R Soc London Ser B 183: 421-425

43. MITCHELL P (1966) Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. Bodmin, UK: Glynn Research.

44. NORDQUIST DT, KOZAK CA, ORR HT (1988) cDNA cloning and characterization of three genes uniquely expressed in cerebellum by Purkinje neurons. J Neurosci 8: 4780-4789

45. PETERSEN OH, GERASIMENKO OV, GERASIMENKO JV, MOGAMI H, TEPIKIN A (1998) The calcium store in the nuclear envelope. Cell Calcium 23: 87-90

46. PHILIPSON KD (1999) Sodium-calcium exchange. In: CARAFOLI E, KLEE CB (eds) Calcium as a Cellular Regulator. Oxford: Oxford University Press. pp: 279-294

47. POZZAN T, RIZZUTO R, VOLPE P, MELDOLESI J (1994) Molecular and cellular physiology of intracellular Ca2+ stores. Physiol Rev 74: 595-636

48. PUTNEY JWJ, MCKAY RR (1999) Capacitate calcium entry channels. Bioassays 21: 38-46

49. ROSE B, LOEWENSTEIN WR (1975) Calcium ion distribution in cytoplasm visualized by aequorin: Diffusion in cytosol restricted by energized sequestering. Science 190: 1204-1206

50. RINGER S (1883) A further contribution regarding the influence of different constituents of the blood on the contraction of the heart. J Physiol (London) 4: 29-43

51. RIZZUTO R, SIMPSON AWM, BRINI M, POZZAN T (1992) Rapid changes of mitochondrial Ca2+ revealed by specifically targeted recombinant aequorin. Nature 358: 325-328

52. RUTTER GA, FASOLATO C, RIZZUTO R (1998) Calcium and organelles: A two-sided story. Biochem Biophys Res Comm 253: 549-557

53. SANTELLA L, CARAFOLI E (1997) Calcium signalling in the cell nucleus. FASEB J 11: 1091-1099

54. SANTELLA L, BOLSOVER S (1999) Calcium in the nucleus. In: CARAFOLI E, KLEE CB (eds) Calcium as a Cellular Regulator. Oxford: Oxford University Press. pp: 487-511

55. SANTELLA L, KYOZUKA K (1997) Effects of 1-methyladenine on nuclear Ca2+ transients in meiosis resumption in starfish oocytes are mimicked by the nuclear injection of inositol 1,4,5 trisphosphate and cADP-ribose. Cell Calcium 22: 11-20

56. SCARPA A, AZZONE GF (1970) The mechanism of ion translocation in mitochondria. 4. Coupling of K+ efflux with Ca2+ uptake. Eur J Biochem 12: 328-335

57. SHANKAR G, DAVISON I, HELFRICH MH, MASON WT, HORTON MA (1993) Integrin receptor-mediated mobilization of intranuclear calcium in rat osteoclasts. J Cell Sci 105: 61-68

58. STAUFFER TP, GUERINI D, CARAFOLI E (1995) Tissue distribution of the four gene products of the plasma membrane Ca2+ pump. A study using specific antibodies. J Biol Chem 270: 12184-12190

59. SUDBRAK R, BROWN J, DOBSON-STONE C, CARTER S, RAMSER J, WHITE J, HEALY E, DISSANAYAKE M, LARREGUE M, PERRUSSEL M, LEHRACH H, MUNRO CS, STRACHAN T, BURGE S, HOVNANIAN A, MONACO AP (2000) Hailey-Hailey disease is caused by mutations in ATP2C1 encoding a novel Ca(2+) pump. Hum Mol Genet 9: 1131-40

60. TAKESHIMA H, NISHIMURA S, MATSUMOTO T, ISHIDA H, KANGAWA K, MINAMINO N, et al. (1989) Primary structure and expression from complementary cDNA of skeletal muscle ryanodine receptor. Nature 339: 439-444

61. TIMOURIAN H, CLOTHIER G, WATCHMAKER G (1972) Cleavage furrow: calcium as determinant site. Exp Cell Res 75: 296-298

62. TOMBES RM, SIMERLY C, BORISY GG, SCHATTEN G (1992) Meiosis egg activation and nuclear envelope breakdown are differentially reliant on Ca2+ whereas germinal vesicle breakdown is Ca2+ independent in the mouse oocyte. J Cell Biol 117: 799-811

63. TOYOSHIMA C, NOMURA H (2002) Structural changes in the calcium pump accompanying the dissociation of calcium. Nature 418: 605-611

64. VASINGTON F, MURPHY JV (1962) Ca2+ uptake by rat kidney mitochondria and its dependence on respiration and phosphorylation. J Biol Chem 237: 2670-2677

65. WA H (2000) Crystal structure of the calcium pump of sarcoplasmic reticulum at 2.6 Å resolution. Nature 405: 647-655

66. WAGENKNECHT T, BERKOWITZ J, GRASSUCCI R, TIMERMAN AP, FLEISCHER S (1994) Localization of calmodulin binding sites on the ryanodine receptor from skeletal muscle by electron microscopy. Biophys J 67: 2286-2295

Corresponding author: Ernesto Carafoli, Department of Biochemistry, University of Padova and Venetian Institute of Molecular Medicine (VIMM) 35121 Padova, Italy. Phone: (39-049) 827-6137, Fax: (39-049) 827-6125, E-mail:

Received: January 26, 2004. Accepted: March 15, 2004.


Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons