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Biological Research

Print version ISSN 0716-9760

Biol. Res. vol.40 no.1 Santiago  2007

http://dx.doi.org/10.4067/S0716-97602007000100001 

 

Biol Res 40: 5-12,2007

ARTICLES

 

Quantitative genetic variation of metabolism in the nymphs of the sand cricket, Gryllus firmus, inferred from an analysis of inbred-lines.

 

ROBERTO F NESPOLO1, LUIS E CASTAÑEDA1 and DEREK A ROFF2

1Instituto de Ecología y Evolución, Facultad de Ciencias, Universidad Austral de Chile, Casilla 567 Valdivia, Chile; 2 Department of Biology, University of California, Riverside, CA 82521, USA.


ABSTRACT

Compared with morphological and life history traits, quantitative genetic variation of metabolic and related traits in animals has been poorly studied. We used flow-through VC02 respirometry and simultaneous activity measurement on nymphs of the sand cricket {Gryllus firmus) from inbred lines to estimate broad-sense heritability of four metabolic variables. In addition, we measured a number of linear dimensions in the adults from the same inbred lines. There were significant multivariate effects of inbred lines for all traits and broad-sense heritability for physiological traits was 4.5%, 5.2%, 10.3% and 8.5% for average, resting, minimum and maximum C02 production in nymphs, respectively. Though the MANOVA indicated significant genetic variation among inbred lines in adult morphology, the broad-sense heritabilities were relatively low ranging from 0-18%. Our results indicate that the heritabilities of metabolic measures are large enough to potentially respond to selection.

Key terms: broad-sense heritability, metabolic rate, insects, inbred lines, through-flow C02 respirometry.


INTRODUCTION

The potential to respond to natural selection depends on the heritable variation of a trait (Falconer and Mackay, 1997; Lynch and Walsh, 1998). A measure of this variation is heritability, the ratio between the additive genetic and phenotypic variances. Traits can be classified as morphological, physiological, life historical and behavioral (Mousseau and Roff, 1987; Hoffman, 2000). The amount of genetic variation in these types of traits, as well as in different kind of organisms, has been the subject of considerable research and debate (Roff and Mousseau, 1986; Mousseau and Roff, 1987; Price and Schluter, 1991; Houle, 1992; Roff, 1997). Overall, the heritabilities of physiological traits are similar to life history and behavioural traits but lower than morphological traits (0.31, 0.26, 0.37 and 0.51 for physiological, life history, behavioural and morphological traits respectively. Data from table 3 of Mousseau and Roff [1987]). In Drosophila the heritability of physiological traits appears to be somewhat higher and comparable to morphological traits (Roff and Mousseau, 1986; Hoffman, 2000). However, whereas there are numerous heritability estimates of morphological (n=140) and life history traits (n=79), there is a relative scarcity of heritability estimates for physiological traits (n=39, data from table 3 of Mousseau and Roff [1987]). The distribution of estimates is very uneven, with a few animals such as Drosophila, mice, humans and several bird species having been relatively well studied (Mousseau and Roff, 1987), but the majority of species being virtually unknown from any quantitative genetic perspective. Further, physiological traits are not functionally consistent across species. For some animals, many physiological traits (e.g., dessication resistance in insects, Hoffman, 2000) are functionally very different than traits classified as "physiological" in other animals (e.g., oxygen carrying capacity by haemoglobin in mammals, Chappell and Snyder, 1984). Hence, it is likely that estimates of the heritability of particular categories of physiological traits cannot be readily used to infer values in other categories. Thus the scarcity of estimates and the breadth of variation within physiology make it difficult to reach any conclusions with respect to the range of heritabilities in this type of trait. For instance, in Drosophila heat resistance, desiccation resistance and starvation are classified as physiological traits but h2 ranges from 0.18 to 0.9 with considerable variation across studies (Hoffman, 2000). Similarly in mammals, body temperature, thermal conductance and basal metabolic rate exhibit a 100% variation in heritabilities (Nespolo et al., 2003,2005).

These complications in the analysis of the heritability of physiological traits could be avoided or at least ameliorated by: (1) comparing estimators of different traits obtained from experiments in the same individuals, (2) considering a common physiological trait in very different organisms (e.g. metabolic rate, a general proxy of the "intensity" of functioning of every metacellular animal, represents nearly the same state variable in very different animals). The repeatability of standard metabolic rate is high in several species of insects (Rogowitz and Chappell, 2000; Nespolo et al, 2003a; Terblanche et al, 2004), which is consistent with a high heritability. On the other hand, both broad-and narrow-sense heritabilities of metabolic rate appear to be relatively low in vertebrates. For instance, Dohm et al, (2001) presented only negative estimates for Mus, Nespolo et al (2003b) report h2 = 0.14 (non-significant) for basal metabolic rate in a field rodent. The purpose of the experiments reported herein was to determine if the broad-sense heritability of metabolic rate in the sand cricket, Gryllus firmus is consistent with the high repeatabilities estimated in invertebrates or with the low heritabilities reported in vertebrates. Specifically, we used flow-through VC02 respirometry to measure the metabolic rates in the nymphs of four highly inbred lines of G. firmus. To gain resolution, we extracted several metabolic variables from the VC02 record, and compared after scaling with morphological variables. Since morphology of adults of these inbred lines is known (Roff and Sokolowska, 2004), we also measured morphological traits as control.

MATERIAL AND METHODS

Insects

The inbred lines used in the present experiment were derived from a stock culture that originated from approximately 20 males and 20 females collected in northern Florida in 1981. The stock culture is maintained with a standing adult population of several hundred individuals (generally 100-500 adults, with occasional bottlenecks in which the population may have declined to about 50 adults). To prevent diapause the temperature is maintained in excess of 25°C. Nymphs and adults in both the stock and the experiment were fed Purina rabbit chow.

The inbred lines were created from the stock population by 14 generations of brother-sister mating, full details of which are given in Roff (2002). After generation 14 the seven extant inbred lines were maintained separately in the same manner as the stock culture. Three inbred lines were lost due to accident leaving four extant lines at the time of the present experiment. As with the original stock, the inbred lines are maintained at population levels of several hundred per cage, with two cages per inbred line. A total of 120 nymphs were drawn haphazardly from the stock cages of the four inbred lines (30 per line), with approximately equal numbers coming from each of two cages per line. Densities within the cages were not controlled and hence heritabilities obtained in the present analysis are likely to be reduced relative to previous analyses in which initial densities were controlled.

Morphometric measurements

In all individuals (15 adults and 30 nymphs per line), in addition to body mass, we measured head width, head length, prothorax length, and abdomen length. Whereas body mass changes with adult age, all the other measures remain constant after eclosión into the adult morph.

Respirometry

Our respirometry system was similar to Lighten and Turner (2004) and Rogowitz and Chappell (2000). In brief, carbon dioxide production was measured continuously with an infra-red C02 analyzer (LI-COR LI6251) capable of resolving differences of 0.2 part per million (ppm) of C02 in air. The analyser was calibrated periodically against a precision gas mixture (there was almost no drift between calibrations). Flow rates of dry, C02-free air were maintained at ±1% by a Sierra mass flow controller. Air was drawn from the ambient, vapour and C02 water scrubbed with a Drierite-Ascarite column, and flow rate controlled at 95 ml miir1. The metabolic chamber was a 40 ml glass-cylinder. To monitor movement we used a Sable System AD-1 activity detector. Each cricket was measured during 45 minutes but we considered only the last 20 minutes. Each record was automatically transformed by a macro program recorded in the Datacan software (Sable Systems), in order to (1) correct the six-second lag introduced by the distance between the analyzer and the chamber and then to match the activity record with the VC02 record, and (2) to transform the measure from parts per-million to ml-C02 per hour, taking into account the flow rate. For this, we used the standard equation when flowmeter is downstream and C02 is scrubbed prior to flow measurement: VC02 = STP * (FeC02 - FiC02) * FR / (1 - FeC02 + FiC02/RQ), where STP = standard temperature and pressure correction (equal to one with mass-flow controllers), FeC02 = excurrent fractional concentration of C02 , FiC02 = incurrent fractional concentration of C02 , FR = flow rate, RQ = respiratory quotient, which for herbivorous animals is assumed to be 0.85.

We took four respirometric variables from each individual: average, resting, maximum and minimum rates. As a "general" measure of metabolism, we took the complete average of each transformed record (=VC02avg). In addition, we used the activity measurements to detect "resting" periods in each record. Most of the individuals presented a fairly clear pattern of resting and active periods, revealed by the conspicuous drops in VC02 during periods of undetectable activity (Fig. 1). Resting metabolism (VC02rest) was computed as the average of the one-minute steady state VC02 consumption during periods of inactivity. In addition, we considered the single maximum (VC02max) and minimum (VC02min) values of VC02 during the record.


Figure 1: Representative record of C02 production (black line) and activity measurement (red line, arbitrary units). Black bars at the bottom denote periods of inactivity, evidenced by the match between low activity and low VCO2

The usual approach for the analysis of VC02 or V02 times-series is either to take averages, single values, or a combination of both for parts of whole records. As described above, we followed such procedures but we believe that some information, related to the pattern of peaks and valleys of the record itself is not detected by this procedure. A common approach to capture quantitatively this kind of pattern from time-series is Fast Fourier Transform (FFT). In brief, FFT is used to produce frequency analysis of discrete non-periodic signals. For the case of metabolic time-series, FFT permits adjustment of the peaks and valley structure of the record to a plot of amplitude versus frequency. By this procedure, noisy records (i.e., records with many small peaks of high frequency) produce FFT plots with a low slope (Fig 2). Records with a larger variety of peaks (i.e., big peaks and drops, combined with environmental noise) produce a larger (negative) slope of the FFT plot (Fig. 2). Consequently, in addition to the effects of inbred lines on the magnitude of the different measures of VC02 described above, we examined the variation in the slope of FFT in relation to body mass.


  Figure 2: Comparison of FFT plots between a very noisy record (A) and a record with a great variety of medium and large peaks (B).

Statistics

For adults we had two main categorical predictor variables, sex and inbred line, and a single continuous variable, body mass. Because in small nymphs sex cannot be determined, we did not record sex for the nymphs. Broad sense heritabilities were estimated using variance components estimated from a one way analysis of variance. Approximate standard errors for the estimates were computed from the intraclass correlation coefficient variance for full sibs (Falconer and Mackay 1997, pp. 180). Additionally, we tested for overall variation among lines in morphology using MANOVA. We log transformed the metabolic measurements to comply with the assumptions of ANOVA. Heritability estimates were made for the adult morphological measurements but, because the age of the nymphs was unknown and linear measurements change with nymphal age, we did not estimate heritabilities for these traits. However, because body mass is expected to have a large phenotypic effect on metabolic rate, we estimated the heritability of the metabolic variables after correction for body mass effects.

RESULTS

Adult morphology

The MANOVA indicated highly significant variation among lines in overall morphology (Wilks λ = 0.469, F18; 1447=2.465, P = 0.0016, Table 1) but no single trait, after the Tukey a-posteriori test, was significantly variable among lines (Table 1). This was also reflected in low broad-sense heritabilities for some traits: h2 = 0, 0.02 ± 0.04, 0.05 ± 0.06, 0.18 ± 0.11, (prothorax length, head width, head length, and prothorax width, respectively). These results indicate that there is genetic variation among lines in morphology, as found previously, but some of the estimations were considerably small (Roff and Sokolovska 2004).


Nymphal metabolic rate

There were significant effects of inbred lines on the four respirometric variables (Wilks'λ, = 0.787, F12,228 = 1.8, P = 0.049, MANCOVA with body mass as a covariate, Table 2). The Tukey post-hoc test, revealed significant differences among inbred lines in all variables (Table 2). The broad-sense heritabilities were 0.045 ± 0.04 for VC02avg, 0.052 ± 0.06 for VC02rest, 0.10 ± 0.06, for VC02min and 0.085 ± 0.05 for VC02max. The slope of FFT was significantly correlated with body mass (r = 0.83 P= 0.001) but there were no significant effects attributable to inbred line (F3,115 = 1.22; P = 0.30, ANCOVA).


DISCUSSION

One of the least known aspects of insect physiology is the genetic variance of physiological traits, especially metabolic rate (Hoffmann, 2000; Nespolo et al, 2003a). This is rather surprising as flow-through respirometry has been intensely used to study a very wide range of factors correlated with metabolic rate, such as age (Hack, 1997), diet composition (Zanotto et al, 1997), reproductive cost (Prestwich and Walker, 1981; Hack, 1997, 1998), flight and locomotion (Rogowitz and Chappell, 2000), and adaptive features such as seasonal acclimation (Forlow and MacMahon, 1988), dry habitat adaptations (Cooper, 1993), altitude (Hadley and Massion, 1985), latitude across populations (Rourke, 2000) and species differences (Davis et al, 2003). All these patterns of physiological adaptation are potential consequences of past genetic variation in metabolic rate, making genetic variance a very important quantity. But, so far as we are aware, no study has addressed the heritability of metabolic rate. Repeatability studies suggest that metabolic rate is consistent over time in insects, and is consistent with a high heritability (Nespolo et al, 2003a; Chappell and Rogowitz, 2000; Terblanche et al 2004). The low broad sense heritabilities we found in morphological traits of adults (0-0.18) are lower than obtained in previous studies in adults of the seven inbred stocks, (Roff and Sokolovska 2004). This fact could be in part explained by the reduction sample size and because of the absence of density control in the cages (see methods). It is known that rearing conditions such as density and temperature could affect adult morphology in insects (Begin et al., 2004). This effect in cages would inflate the environmental variance thus reducing computed heritability. Nevertheless, the MANOVA did demonstrate genetic variation for overall adult morphology among the four inbred lines. Given the low variance in the morphological traits, the heritabilities of metabolic rate measures (0.04-0.10) are perhaps underestimated, but this observation is only qualitative. For the case of FFT slopes (i.e., the pattern of the metabolic time-series), no genetic effects were detected, but a strong body mass dependence. The biological interpretation of this finding is that larger individuals exhibit also larger peaks and valleys than small ones, which exhibit more continuous patterns. These bursts of C02 in large crickets could be due to some form of cyclic gas exchange, such as in carabid beetles and moth pupae (Chown et al., 2006). However, this needs to be confirmed since no cricket has exhibited DGC to date. Overall, these data show that metabolic rate is an "evolvable" trait within populations, but further experiments are required to determine how much of the variance is due to additive effects. This picture is somewhat inconsistent with the information available from other animals: mice and wild rodents appear to have a very low heritability of metabolic rate (negative estimate in Dohm et al 2001; 0.14 in Nespolo et al 2003b; 0.01 in Bacigalupe et al 2004). Also, physiological traits other than metabolic rate (i.e., heat and cold resistance, ethanol resistance and dissecation resistance) in Drosophila present narrow-sense heritabilities below 0.2 (Hoffman, 2000). However, as we advanced in the introduction, physiology is a very heterogeneous category and generalizations within this category may be misleading. The difficulties of measuring metabolic rate in animals have impeded accurate estimates of heritabilities, as reflected in generally low sample sizes: for example, the cited studies above were all performed using sample sizes of less than 26 families (Dohm et al 2001; Nespolo et al 2003b; Bacigalupe et al 2004). Estimates of heritabilities of metabolism in mammals obtained with larger sample sizes appear to give large and significant narrow-sense heritabilities (Nespolo et al., unpublished results; Koteja, personal communication).

The existence of genetic variation in metabolism in the nymphs of the sand cricket suggests that selection over energy processing capacities would generate an evolutionary response (although it will depend on the magnitude of the selection differential). The implications of this are important for several reasons. Energy consumption during larval period is a proxy for energy processing efficiency (Gouveia et al 2000), which in turn determines growth rate and possibly adult size. Given the tradeoff between growth rate and reproduction (Roff, 2000), it would be reasonable to hypothesize that the metabolic rate of the nymphs could be a determinant of the reproductive output of adults (eg., larger females could produce more eggs and larger males could have an advantage in time spent calling and hence attracting females, Roff, 2000). An alternative scenario is an energetic trade-off, with a higher metabolic rate reducing the energy allocated to growth. In adult crickets, energetic trade-offs have been observed in the cost of wing maintenance, where macropters due to their massive flight muscles have a higher metabolic rate than micropters, and divert less energy to gonads (affecting fecundity) (Crnokrak and Roff, 2002). Such a trade-off could not occur in the nymphs as they do not have wings and mature gonads, but metabolic rates in the nymphs may translate into constraints in the adults via genetic correlations between metabolic rates at different ages. The important finding of the present study is that metabolism (or energetic efficiency) is genetically variable and hence can be a target of natural selection.

 

ACKNOWLEDGEMENTS

This work was partially supported by a MECESUP fellowship and Fondecyt grant No 3030032 to Roberto Nespolo, and funds supplied to DAR by an initial complement grant from UCR, to Derek Roff. Luis Castañeda acknowledges a Conicyt fellowship.

 

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Received: November 16, 2005. Accepted: May 30, 2006

 

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