INTRODUCTION
In recent years, with the continuous development of Micro-Implanted-Anchorage (MIA) materials and technology, MIA in the use of orthodontic increasingly common (Topouzelis & Tsaousoglou, 2012; Sivakumar & Sivakumar, 2014; Khan et al., 2016). Skeletal class II malocclusion patients after removal of premolars, through the MIA retracting anterior teeth to achieve the effect of treatment (Gandhi et al., 2017; Wu et al., 2017). The initial stability of the microscrew implantis closely related to the thickness of the surrounding cortical bone. Studies have shown that the thickness of the cortical bone is at least 1 mm to achieve good results. MIA implantation location and the angle of the different, the thickness of the bone cortical bone will be different, and thus affect the stability of microscrewimplant (Jasoria et al., 2013; Kalarickal et al., 2014). When the implantation site for the oblique bone surface (such as zygomatic alveolar crest, retromolar pad, external oblique ridge, etc.), the implanting nails may slide along the periosteum within the mucous membrane, without entering the cortical bone and slipping off. Incorrect implantation angle can cause the cortical thickness to be too small and cause the MIA to fall off. Therefore, the choice of appropriate implantation site and implantation angle is an important factor affecting the success of MIA. In this study, CBCT measurements of cortical bone thickness at different angles of different height in the maxillary posterior region, which provided a reference for the safety of MIA implantation in Skeletal class II malocclusion.
MATERIAL AND METHOD
Materials selection. CBCT images of 20 patients (10 males and 10 females) were selected from September 2016 to August 2017 in the Department of Radiology, Shanxi Medical University Stomatological Hospital. Inclusion criteria: 1) ages, 18-40 years; 2) skeletal class II malocclusion; 3) ANB≥4.7; 4) maxillary dentition complete (except for the third molar). Exclusion criteria: 1) history of cleft lip and palate, orthodontic treatment or trauma; 2) oral and maxillofacial deformities and other systemic diseases affecting oral and maxillofacial development; 3) maxillary sinus lesions such as cysts, tumors and so on; 4) maxillary sinus floor height less than 8 mm.
Instruments and software. Oral and craniofacial CT (KAVO, German); Invivo 5.0 software; SPSS17.0 software.
Acquisition of CBCT image data. The maxilla was scanned with KAVO 3D eXamVision CBCT, and the scan data was converted to DICOM format.
Three-dimensional image reconstruction. The CBCT scan data was introduced into the In vivo 5 analysis software in a DICOM 3.0 standard file for multi-level reconstruction with a thickness of 0.25 mm layer, including reconstruction and 3D surface reconstruction. Adjust the sagittal plane, the coronal plane and the cross section so that the horizontal observation line and the vertical observation line pass through the alveolar crest and divide the apical area (Fig.1).
Measurement index. The distance from the alveolar crest 4 mm, 6 mm, 8 mm identified as fixed point (Fig. 2); 1) thickness of the cortical bone and its maximum bone mass at the angle of 45°, 60° and 90°, respectively, on the three fixations (Fig. 3); 2) distance between the alveolar crest and the maxillary sinus floor. All measurements were measured by the same person every 2 weeks, a total of three times, and the results were averaged.
Statistical Analysis. SPSS17.0 statistical software was used to analyze the data, and the one-way ANOVA and LSD method were compared.
RESULTS
There was a significant difference in the thickness of the cortical bone obtained by implanting MIA at the different height and different angles, it has statistical significance (P < 0.05). At the same height, the greater the inclination angle of the implanted MIA, the thicker the cortical bone (I >II > III). There was a significant difference in the thickness of cortical bone obtained by implanting MIA at the same angle of different heights, it has statistical significance (P<0.05), the higher the implant site, the thicker the cortical bone thickness a<b<c) (Table I).
There was not a significant difference in the maximum bone mass obtained by implanting MIA at the same height and different angles, it has statistical significance (P<0.05). There was not a significant difference in the maximum bone mass obtained by implanting MIA at the same angle of different heights (P < 0.05) (Table II).
Annotations: I, II, and III represent the 45°, 60°, 90° angle of implanted MIA; a, b, c, represent the distance from the alveolar crest distance of 4 mm, 6 mm, 8 mm;* represent a significant difference between groups (P <0.05).
Table I Measurement of cortical thickness at different angles from alveolar crest at different heights (mm, X±Sn=20).

DISCUSSION
With the widespread application of MIA technology, reasonable and personalized implantation of micro-implant nails are more and more orthodontist attentive. The implantation of microscrew implant requires a certain thickness of cortical bone support, and the implantation site and angle are different, resulting in a different thickness of the cortical bone, which in turn affects the stability of MIA (Lee et al., 2014; Maino et al., 2016; AntoszewskaSmith et al., 2017). From the safety point of view that the implantion position from the junction cemento-enamel farther, the thicker the cortical bone, the greater the risk. The most suitable implant site is from the junction cemento-enamel 2-4 mm, implanted angle greater than 45° to avoid touch the maxillary sinus. Some scholars from the perspective of reducing micro-implant inflammation around the point of view, that the maxillary posterior región of the microscrew implant should be in mucogingival junction or attached gingival, that is, from the alveolar crestabout 4 mm (Tenenbaum & Tenenbaum, 1986; Melsen et al., 2015; Lee et al., 2017). Some scholars measured the cortical thickness of the maxillary posterior root tip to the alveolar crest at 2 mm, 4 mm, 6 mm, 8 mm level, and suggested that the cortical thicker from the alveolar crest can obtain good initial stability, but there is no combination of angle to measure (Duran et al., 2015). In this study, the thickness of cortical bone and bone was measured at 45°, 60° and 90° from the three sites of the alveolar crest at 4 mm, 6 mm and 8 mm, respectively. Measurement of cortical bone thickness and implantation angle in the maxillary posterior region was used to provide reference for the safety of MIAimplantation in skeletal class II malocclusion.
Table I shows that at the same implant angle, the thickness of the cortical bone at 8 mm was significantly greater than at 6 mm and 4 mm. That is, the alveolar crest at the top of the cortical bone is thin, away from the alveolar crest near the base bone bone cortex thicker. The maxillary and mandibular first molars of 43 adult patients were measured from the alveolar crest respectively in the direction of the apical direction. It was found that the thicker the cortical thickness was obtained from the alveolar crest, the greater the mandible than the maxilla (Ono et al., 2008). For the implanted area of patients with adequate bone, can choose to stay away from the alveolar crest site to implant MIA. At the same implantation height, implanted MIA with a tilt angle of 45º to 60º, 90º to obtain the best cortical bone thickness. Laursen et al. (2013) experimented with the implantation of MIA from 90° to 45°, and the contact area of the cortical bone increased by 47 %. The results are consistent with this study (Laursen et al.). Wilmes et al. (2008) from the perspective of torque, displacement stability of the study, also reached a similar point of view. Therefore, when the amount of bone from the maxillary sinus sufficient, the MIA can be selected with a larger angle implantation.
In summary, the greater the thickness of the cortical bone in the maxillary posterior region of skeletal class II malocclusion, the greater the thickness of the cortical bone. The larger the tilt angle is, the thicker the cortical bone is obtained from the alveolar crest. It is advisable to choose a small angle (45°-60°) to implant a MIA or to select a slightly longer micro-implant at a location near the alveolar crest (near the alveolar crest 4 mm). In the case of not touching the maxillary sinus, you can choose to stay away from the alveolar crest site implantation, in order to obtain greater bone cortical thickness in order to facilitate the stability of microscrew implant.