INTRODUCTION
Being a mother requires maternal functions such as nursing and breastfeeding pleasure; or else, it is impossible to be a mother in all aspects. Although maternal pleasure has been explained by various neuro-behavioral, philosophical, social, and even theological theories, no satisfying mechanism has been found so far. It is easily hypothesized that breastfeeding pleasure created by a neural network should be available in that maternal biological organization. For example; thousands of papers have argued that stimulation of tongue taste buds provides to food choosing, recognizing, and ingestion, which essential for living. The more interesting one, all animals have propagation instinct; but, unless orgasmic sensations, is it possible that sexual relationship is essential for reproduction? Lately, Aydin et al. (2014) have discovered taste bud-like structures essential for creating orgasmic sensation and stimulated by seminal fructose, in penile tissues. Aydın et al. (2018), have described that the presence of taste rosea stimulated by seminal fructose via pudendal nerves in the urethra and glans penis in rats. If so, mammary tissues secreting various sugars such as sucrose, fructose, glucose, and many useful chemicals (Blass & Shide, 1994) should have taste rosea to initiate and continue maternal actions and behaviors. According to taste rosea related literature, we thought that taste bud-like structures could be available in the mammary glands to provide breastfeeding pleasure of mothers. In our study, we found taste bud-like structures in mammary glands of breastfeeding mothers such as those in the tongue and urethral tissues. Because they are similar to rosea bundle, the newly discovered taste buds of breast were named as taste rosea by own ourselves, and this is the first description in the literature.
MATERIAL AND METHOD
Animals Selection. In this study, twenty-two female Sprague Dawley rats weighing 320-400 g were chosen. Animals were purchased from the Experimental Animal Laboratory of Ataturk University School of Medicine. The rats were housed in standard metal cages and they kept an air-conditioned room with standard humidity under 12/12 hours light/dark cycle. All animals followed for six mating periods, and most of them become pregnant during that period. Rats were subsequently divided into three groups as control (n=6), pregnant (n=6) and breastfeeding (n=10) groups. To observe if there are taste-bud-like structures in breasts, they were sacrificed under general anesthesia, and histopathological examinations were carried out.
Histologic Procedure. The breast and tongue tissues of all subjects were removed. After fixation in 10 % neutral formalin solution, the tissues were passed through alcohol and xylol series and embedded in paraffin blocks. First, tongues and nipples divided horizontally into 10 segments, and then about 20 sections were cut from each block 5mm in thickness with a microtome (Leica RM2125RT, Leica Microsystems, Wetzlar, Germany). Some specimens were stained with Hematoxylin & Eosin (H&E) and modified aldehyde fuchsin stain. Under the light microscope (NikonEclipse E600, Japan), the anterior parts of tongues and nipples of all animals were examined histologically to compare with each other.
Because of Gustducin antibody recognizes taste cells in taste buds, other sections were stained by using immunostaining techniques in order to detect the expression of gustducin in taste buds in tongue and breasts. The sections were placed on immunohistochemistry device (Leica, BonMax), and then processes of deparaffinization, dehydration, antigen retrieval and blocking of endogenous peroxidases were performed. Primary antibody (Anti-gustducin LifeSpan BioSciences, Inc) was dropped on the sections for 60 min. After washing with phosphate buffer solution, sections were applied a secondary antibody and following, DAB+chromogen was added and waited until the brown color was observed. Subsequently, slides were stained with hematoxylin for 5 min and passed through alcohol and xylol series. At the end of the protocol, slides were removed from the device, mouthed with coverslips and evaluated under the light microscope.
Breast Taste Rosea Number Estimation Method. The sections stained with Hematoxylin & eosin stain and antigustducin antibody were examined under the light microscope. To estimate number of taste rosea, the mean lumen diameter of internal ducts in each 20 consecutive sections were shown with ri1,ri2,ri3,…,ri20; and mean external radius values were shown as re1+ re2+re3….re20. The length of the duct was shown as h = h1 +...h20=Σh and accepted as the total height of the cylinder-shaped channel. The volume values of all channels were calculated based on the following formula: V=ΣΠη(re2 - ri2). Taste rosea density (d= n/Duct). The total number of taste rosea/all nipples: ΣΠh=dΣΠη(re2 - ri2) was determined by using physical dissector methods as in our previous article (Aydin et al., 2014; Aydin & Aydin, 2015).
Statistical Analysis. Results were analyzed by using SPSS 21.0 for Windows. Multiple comparisons among groups were done by Kruskal Wallis test. Using the Mann-Whitney U test, binary comparisons between groups were assessed. Values are given as the mean±standard deviation. P-value <0.05 was accepted significant.
RESULTS
Histological Results. The breast taste rosea were examined with taking reference of the lingual taste buds of each animal. Taste buds were located as an intraepithelial sensory organ, especially in circumvallate papillae in the tongue. They are pale stained structures extending along with the epithelium thickness, and their function is to receive taste sensation.
General Features of Taste Buds. Breast taste rosea were located immediately beneath the epithelium as embedded in the connective tissue surrounding the duct. In the light microscopic view, they appeared slightly paler stained than the epithelial cells, and their cells were lined up vertically to the duct epithelium. In the H&E stained sections, cell clusters resembling the taste bud were located close to the secretory units of the mammary gland, and each breast taste rosea was surrounded by a delicate connective tissue. Breast taste rosea cells send membranous fibers to neighboring cells among the breast taste rosea cells and their nerves. In order to determine if there were breast taste rosea in breast tissues, gustducin antibody was used. In specimens, neuronal fibers driving from the taste rosea, which resemble those of the tongue, were observed in all breast taste rosea in the ducts. A small nerve fasciculus that penetrates the basal lamina as spiral convolutions, and cell clusters of the breast taste rosea, same as lingual taste buds were observed with gustducin immunohistochemical staining. The special characteristics of newly described breast taste rosea were summarized in the legends of Figures 1 to 5.
Numerical results. The number of breast taste rosea of lactiferous ducts and statistical significance is as follows: The number of breast taste rosea nearly was 1 or 3 in each cubic millimeter on average, whereas in the distal part of the duct opened to the nipple was 4-8/mm3. Breast taste rosea numbers were estimated as 3±1/mm3 in virgins, 167±27/mm3 in pregnant and 375±63/mm3 in breastfeeding animals. Between virgins/pregnant, pregnant/ breastfeeding, and pregnant/breastfeeding, the p values were p<0.005, p<0.0005, and p<0.00001, respectively.

Fig. 1 Histological appearances of the H&E stained mammary gland in a virgin rat at low and (A, in the green frame, H&E x10) and high magnification (Base, H&E x 20).

Fig. 2 Histological appearances of the mammary gland in a pregnant rat at low and (A, in the green frame, H&E x10) and high magnification (Base, H&E x 20). The arrow indicates the breast taste rosea (TR).

Fig. 3 Histological view of taste rosea of the tongue (A, white arrow, H&E, x10), lactiferous duct (LD) with breast taste rosea (TR) (Base, H&E, x 20) and magnified appearances of breast taste rosea (B, red arrow, Gustducin immunoreactivity, x40) of a breastfeeding rat. TR, breast taste rosea.

Fig. 4 Histological view of taste rosea (green frame) and lactiferous duct (A, H-E, x10), and magnified appearances of breast taste rosea (TR, yellow arrows) (Base, H&E, x 20) of a breast-feeding rat. Another view of taste rosea in B (H&E, x40).
DISCUSSION
The hedonic pleasure mechanism of breastfeeding mothers has not yet been clarified, although there is an essential similarity between taste sensation and hedonic feeling during breastfeeding. In this study, we examined the mammary tissues of breastfeeding rats to investigate whether there was breast taste rosea in lactiferous ducts of breastfeeding mothers.
Taste buds are composed of dark, light, intermediate and basal cells (Suzuki, 2007). The cells of breast taste rosea were similar to intermediate and basal cells. Although the relationship among these cells types is not clear, most authors have expressed that basal cells form to dark cells, and they mature into light cells, light cells then transform to intermediate cells. For reception of sweet, umami, and bitter taste sensations, these cells have taste receptors. Nerve fibers entering into the taste buds form synapses with the taste cells called neuroepithelial cells, and taste sensation is carried to the brain (Gartner, 2015). Taste receptors might be found in intestinal taste sensing cells reacted with a-Gustducin loss in severe colitis (Feng et al., 2018). Karadeniz et al. (2019) showed that olfactory impulses have important effects on mammary gland functions and cytoarchitecture.
Aydin et al. (2014) discovered urethral taste bud resembling cell clusters - such as tongue- awakening with seminal fructose and creating/conveying orgasmic sensation to the central nervous system. Aydın et al. (2018) have described that there are taste rosea stimulated by seminal fructose and innervated by pudendal nerves in the urethra and glans penis in rats. In a recent study, fructose solution was injected into the urethral orifices of rabbits, and the relationship between penile erection and pudendal nerve ganglia was examined. At the end of the study, the existence of the positive correlation between these two situations has been determined. Moreover, it has been reported, that seminal fructose might stimulate taste rosea of the urethra and glans penis via pudendal nerves (Aydin et al., 2018).
Human milk contains many types of bioactive molecules, e.g., oligosaccharides, lactoferrin, Ig, growth factors, and cytokines. These molecules play essential roles in the protection against infections, in organ development and immune system maturation (Jantscher-Krenn et al., 2012; Rigo-Adrover et al., 2017). In another study, it has been reported that sucrose, glucose, and fructose have calming and pain-relieving effects in rats (Blass & Shide, 1994). In addition, we have thought that especially oligosaccharides provide the mother’s pleasure from the breastfeeding process, by stimulating the breast taste rosea located in lactiferous ducts.
In humans, the neurochemical mechanism explained by Liu et al. (2017) reported that milk production augmented a breast specific protein synthesized by breast tissue. Microantomical studies have informed that vago-mammarial network stimulation via baby suckling may contribute to milk synthesis and production during breastfeeding (Quigley et al., 2017). The neurohumoral mechanism declared that oxytocin and increased vagal nerve activity, stimulates milk flow and facilitate lactation (Uvnäs-Moberg et al., 2001). However, in rodents, the mammary gland has been supplied by the afferent/efferent fibers of pudendal nerves coming from the sacral plexus (Kirk & Kitchell, 1988). According to the mentioned literature, we easily have drawn the conclusion that parasympathetic network such as vagal/ pudendal nerves could be essential formilk synthesis, production, secretion, flowing, kind maternal behavior, and breastfeeding pleasure.
We detected that the female lactiferous ducts contain many breast taste rosea opened to the lactiferous ducts and had a similar shape of the lingual taste buds. Breast taste buds also have neuron-like extensions at the apical ends of taste buds in the wall of lactiferous ducts. Probably, both of the urethral and breast taste rosea, are innervated by the autonomous pelvic nerves in animals, and vagal nerves in humans, respectively. According to our results, breastfeeding pleasure may originate from breast taste rosea and conveyed with the mentioned nerves to the taste information regulating centers in the spinal cord and brain. We assumed that breast taste rosea stimulated by fructose-galactose found in milk and pelvic-pudendal nerves, may play an important role in the formation of the hedonic sensation of breastfeeding mothers which until now, has not been fully examined.
Readers may wonder if which cellular sensation informed vagal or pudendal nerves about the functions of the breastfeeding mothers. Our response to curious readers is that newly described breast taste rosea, stimulated by milk sugars, is the first stimulated cellular structure. It was shown that taste rosea could be innervated by pudendal nerves in rats. Taste rosea-pudendal network may convey breastfeeding pleasure to spinal cord, milk producing neuroendocrine and other integrative brain centers. In summary, we concluded that the described neural network could regulate function among all milk production related organs.
CONCLUSION
It is estimated that the vagal/pudendal nerve innervates these breast taste rosea and convey the hedonic sensation of breastfeeding mothers from the breast taste rosea to taste information regulating and computing in the higher brain centers which has not been mentioned in the literature so far.
Limitation. If further neuropsychological examinations, biochemical analysis, electrophysiological studies could be performed, the article documents may show better results.
Future Insight. This analysis will open new horizons in the understanding of maternal breastfeeding, rational mechanism of the milk-producing factory, the importance of mother/ infant nutrition, protection of mother/infant life and prevention of psychological, neurological, metabolic, malign and infectious diseases. Taste rosea will have many beneficial effects on mammary gland transplantation, vagal/pudendal nerve stimulation, to restore milk production following various disorders in the future.