SciELO - Scientific Electronic Library Online

vol.29 número2Produção de serapilheira na Caatinga da região semi-árida do Rio Grande do Norte, BrasilNuevos registros de insectos fitófagos asociados a canelo (Drimys winteri Forst.) en La Araucanía índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados




Links relacionados


Idesia (Arica)

versión On-line ISSN 0718-3429

Idesia vol.29 no.2 Arica ago. 2011 

IDESIA (Chile) Volumen 29, Nº 2. Mayo-Agosto 2011, pp. 95-98


Reaction of common bean genotypes to Meloidogyne incognita Race 1

Reação do plantas de feijoeiro a Meloidogyne incognita Raça 1


Fábio R. Alves1; Leonardo N. S. dos Santos1; Willian B. Moraes1; Fernando C. Cosmi1; Pablo D. S. Cabral1; Sebastião Martins Filho2; Frederico De Pina Matta1; Waldir C. Jesus Júnior1

1 Federal University of Espirito Santo, Agriculture Science Center, Plant Production Department, 29500-000, Alegre - ES - Brazil. E-mail:
2 Viçosa Federal University, Center for Mathematical Sciences, Department of Statistics, 36570-000 - Viçosa (MG), Brazil.


The objective was to determine the reaction of 33 Phaseolus vulgaris L. genotypes to parasitism by Meloidogyne incognita race 1.  The genotypes used in this study six included commercially exploited ("IAPAR­- 81", "EL- 22", "Carioca", "Serrano", "BATT- 477" and "Uirapuru") varieties, one susceptible ("Rico- 23") and two resistant ("Aporé" and "Pérola") varieties; the others were provided by farmers of the South of Espírito Santo State. The experiment was carried out in a greenhouse in a completely randomized design with 7 replicates. The plants were inoculated with 4,000 eggs + second-stage juveniles (J2) of nematodes. After 50 days, the final population of nematodes (FP), reproduction factor (RF) and percentage reproduction rate (%RR) were evaluated. The resistance selection was based on the %RR, i.e., 0-25% = highly susceptible (HS); 26-50% = susceptible (SU); 51-75% = little resistant (LR); 76-95% = moderately resistant (MR); 96-99% = resistant (RE); 100% = highly resistant (HR) or immune (IM). Only the genotype "Preto Meia Lua" was highly resistant. The genotypes "Terrinha- 2" and "Mulatinho" were classified as resistant, "EL- 22", "BATT- 477", "Vermelho", "Bate Estrada" and "Paina" as (susceptible) and genotypes "Rico 23" and "Morgado" as highly susceptible.

Key words: Resistance, bean, root-knot nematode.


Objetivou-se com esse trabalho estudar a reação de 33 genótipos de Phaseolus vulgaris L. ao parasitismo de Meloidogyne incognita raça 1 em casa de vegetação. Desses genótipos seis são cultivados comercialmente ("IAPAR­- 81" , "EL- 22" , "Carioca", "Serrano", "BATT- 477" e "Uirapuru"), um é suscetível ("Rico- 23" ), dois são resistentes ("Aporé" e "Pérola") e os demais foram resgatados em propriedades localizadas no Sul do Espírito Santo. O experimento foi conduzido em casa de vegetação em delineamento inteiramente casualisado com 7 repetições. As plantas foram inoculadas com 4.000 ovos + juvenis de segundo estádio (J2) do nematóide. Após 50 dias da inoculação, foram avaliados a população final de nematóides (PF), fator de reprodução (FR) e o percentual de redução do fator de reprodução (% RFR). Os genótipos foram classificados como altamente susceptível (AS), susceptível (SU), pouco resistente (PR), moderadamente resistente (MR), resistente (RE), altamente resistente (AR) ou imune (IM). Apenas o genótipo "Preto Meia Lua" comportou-se como AR. Os genótipos "Terrinha- 2" e "Mulatinho" foram classificados como RE, os genótipos "EL- 22", "BATT- 477", "Vermelho", "Bate Estrada" e "Paina" como SU e os genótipos "Rico- 23" e "Morgado" como AS.

Palavras chave: Resistência, feijoeiro comum, nematóide das galhas.



The bean (Phaseolus vulgaris L.) is cultivated in Brazil + in 5.5 million hectares, but, besides this considerably area, the yields are low compared to other countries (Hungria et al., 2000). Probably, one cause of this reduced production is the action of phytopatogens, as root-knot-nematodes, Meloidogyne spp. (Vieira, 1988).

 Although phytonematodes frequently are not observed by farmers because of its reduced size or to be associated to root system, the consequence of its parasitism can be drastic to bean plants, implying, in some cases, to the plant death (Vieira, 1988; Costa et al., 2001).

According to some authors when bean plants are cultivated in soil highly infested by M. javanica (Treub) Chitwood or M. incognita (Kofoid & White) Chitwood, the yield reduction varies from 50% to 90% (Zaumeyer & Thomas, 1957,  Freire & Ferraz, 1977 and Agudelo, 1980).

Some management methods are used to reduce nematode populations, nevertheless, not always they are applicable in all field conditions. Some of them, as nematicides, are very diffused in agriculture, however, there are dangerous to environment and health human, and are expensive, this way the use of these products are undesirable  (Campos, 1999).

Only resistant varieties are the most economic and practice option to control phytonematodes, many researchers have tried to find resistance genes in bean against root-knot nematodes, however without success (Freire, 1976; Mendes et al., 1978; Menten et al., 1980; Ribeiro & Ferraz, 1983; Carneiro and Ferraz, 1992; Pedrosa et al., 2000;  Simão et al., 2005).

In the search for plants that exhibit this so desirable level of resistance and tolerance to adverse environmental conditions, the man, since its inception, has selected seed plants more rustic and productive (Nass et al., 2001).

Many farmers in the Southern Espirito Santo State have cultivated different bean genotypes for subsistence for a long time; however researchers have never made any scientific assessment of their resistance against root-knot nematodes. This information is crucial because, according to Alves & Campos (2001), environmental conditions such as high temperatures and sandy soils, common in the south of the Espírito Santo, favor the nematodes reproduction.

Material and Methods

The experiment was carried out in greenhouse from February to April 2006 in a completely randomized design with 7 replicates aiming to evaluate the resistance level of bean genotypes rescued in the Southern Espírito Santo against Meloidogyne incognita race 1.

Initially local cultivars of bean plants cultivated for years by farmers were collected in the southern of Espirito Santo State from august to October 2005 in the municipal districts of Alegre, Iconha, Montanha, Marechal Floriano, Mimoso do Sul, Muniz Freire and Vargem Alta.

Twenty-four genotypes were tested, corresponding to "Terrinha- 1", "Macuquinho", "Amendoim Vermelho", "Enxofre", "Terrinha- 2", "Manteigão- 1", "Manteigão- 2", "Painã", "Amarelinho", "Imperial", "Vermelho", "Fortuna", "Rio Doce", "Caeté-Pé-Curto", "Vagem Riscada", "Rosinha", "Bate Estrada", "Baetão", "Morgado", "Mulatinho", "Mamona", "Macuquinho Verdadeiro", "Preto Meia Lua" and "Sangue de Boi", 6 comercial cultivars ("IAPAR­- 81", "EL- 22", "Carioca", "Serrano", "BATT- 477" and "Uirapuru"). One susceptible ("Rico 23") and two resistant ("Aporé and Pérola") cultivars  were included as a control for reference.

Populations of Meloidogyne incognita race 1 were obtained from roots of tomato plants cv. Santa Clara cultivated in bags containing a mixture of soil and sand 1:1 (V:V), previously sterilized in autoclave (121 ºC/2 h in three consecutive days), and maintained in greenhouse (Zauza et al., 2007).

Sixty days after inoculation of tomato plants, the roots were separated from aerial parts, washed, cut into 5 cm pieces and ground in a blender using tap water, obtaining the inoculum. From this suspension three aliquots of 1 mL were taken and the number of eggs + second-stage juveniles (J2) was determinated in a Peters chamber with a binocular microscope.

The bean seeds were germinated in blotters sterilized and placed in a germination chamber with constant temperature of 25 °C until the root emergence. Subsequently, the pre-germinated seeds were transferred to plastic bags containing 2 L of substrate. Each bag received two seeds.

The plant inoculation with nematodes occurred 19 days after planting and, in the previous day, one plant per bag was eliminated. Each plant received an aqueous suspension containing 4000 eggs + J2 of M. incognita race 1, pipetted into the hole on substrate with about 3 to 4 cm deep.

50 days after plant inoculation the final population of nematodes per root system (PF) was determined using the method proposed by Hussey & Barker (1973), modified by Bonetti & Ferraz (1981). This estimate gave support to determine the reproduction factor (RF) calculated by the ratio between the final population and the initial population of nematodes (RF=Pf/Pi), and subsequently the reduction of the reproduction rate (%RR) (Moura & Regis, 1987). The reaction of the hosts is presented in Table 1.

Table 1. Criterion to evaluate percentage of the reproduction rate (%RR)(adopted from Moura & Regis, 1987).

Results and Discussion

In general manner, the genotypes showed low, moderate or no resistance to M. incognita race 1. Over the years many investigators have sought sources of resistance in bean to root-knot nematodes, and often do not succeed (Freire, 1976, Mendes et al., 1978; Menten et al., 1980; Ribeiro & Ferraz, 1983; Carneiro & Ferraz, 1992), which corroborates the results found here. Despite these results, it is noteworthy that in the present work three cultivars present a good potential to be assessed in future works.

The genotypes used as standard nematode-resistance ("Aporé" and "Pérola") were MR (Table 2). As the limit for change of classification from R to MR is close, it can be stated that the information about the level of resistance for these genotypes obtained in this study are consistent. Simão et al. (2005) evaluated the behavior of the common bean cv. Pérola and Iapar 81 against the M. javanica and related that both cultivars were efficient hosts. However, the authors did not evaluate these genotypes against M. incognita.

The genotype used as standard nematode-susceptible (Rich-23), behaved as HS (Table 2).

Among commercial genotypes studied two behaved as SU ("EL-22" and "BATT-477) and the others ("IAPAR-81", "Carioca", "Serrano" and "Uirapuru") as MR (Table 2). Simão et al. (2005) evaluated the genotypes Pérola and "IAPAR-81" for resistance to M. javanica and concluded that the first had higher rates of reproduction compared with the second.

With respect to genotypes rescued it was observed great mix of performance against the nematode. Among the 24 genotypes one behaved as HS, three as SU, eleven as LR, six as MR, two as RE. Only The "Preto Meia Lua "genotype was classified as HR (Table 2).

Table 2. Final population (FP), reproduction factor (RF), percentage of the reproduction rate (%RR)
and reaction of 33 genotypes of common bean after 50 days inoculated with Meloidogyne incognita race 1

1/ HR = highly resistant, RE = Resistant, MR = moderately resistant;
LR = little resistant; SU = susceptible, and HS = highly susceptible.

A larger number of genotypes showed high susceptibility compared to resistant or highly resistant. This difficulty to find genotypes R or HR has also been reported by other authors (Mendes et al., 1978; Carneiro & Ferraz, 1992). According to Fuzzato et al. (1994) the sources of resistance aren't the same for different nematode species, being difficulty to obtain multiple resistance, so it is necessary that these genotypes are also tested for other nematode species, such as M. javanica, common in areas where beans are grown (Vieira, 1988).

Since the most of the genotypes used in this study consisted of rustic plants, selected by farmers for decades (Nass et al., 2001), it is possible that sources of resistance to other diseases are present in these materials. So it is important that these genotypes are tested for resistance to other diseases, including those of aerial part, in order to find sources of resistance to pathogens that restrict beans cultivation in the Espírito Santo State and in other regions of Brazil, and indicate these genotypes for breeding programs, so that these potential resistance genes can be incorporated into commercial cultivars.


The results obtained from this and other previously published studies indicate that there are few resistance genes in bean to M. incognita race 1.

Three cultivars present a good potential to be assessed in future works.

In order to confirm the results, this experiment should be carried out again in field conditions.

Literature Cited  

Agudelo, F.V. 1980. Nematodes. In: Schwartz, H.F. & G.E. Galvez (Eds).  Bean production problems: disease insect, soil and climatic constraints of Phaseolus vulgaris. Cali: CIAT. 315-326.        [ Links ]

Alves, F. R.; Campos, V. P. 2001. Efeito do aquecimento do solo na resistência de plantas Meloidogyne incognita raça 3 e Meloidogyne javanica. Nematologia Brasileira, 25(2): 153-162.         [ Links ]

Bonetti, J. I. S.; Ferraz, S. 1981. Modificação do método de Hussey & Barker para extração de ovos de Meloidogyne exigua em raízes de cafeeiro. Fitopatologia Brasileira, 6: 553 (resumo).         [ Links ]

Campos, V. P. 1999. Manejo de doenças causadas por fitonematóides, Curso de pós-graduação à distância: Manejo de doenças de plantas. Lavras: Editora UFLA. 120.         [ Links ]

Carneiro, R. G.; Ferraz, S. 1992. Relação de cultivares de feijoeiro a Meloidogyne incognita raça 3. Nematologia Brasileira, 16(1-2): 35-40.        [ Links ]

Costa, M. J. N. Da; Oliveira, S.; Coelho, S. J.; Campos, V. P. 2001. Nematóides em plantas ornamentais. Ciência e Agrotecnologia, 25(5):1127-1132.          [ Links ]

Freire, F. C. O.; Ferraz, S. 1977. Nematóides associados ao feijoeiro, na Zona da Mata, Minas Gerais, e efeitos do parasitismo de Meloidogyne incognita e M. javanica sobre o cultivar "Rico 23". Revista Ceres, 24(132): 141-149.        [ Links ]

Freire, F. C. O. 1976. Nematóides associados ao feijoeiro na Zona da Mata, Minas Gerais, e aspectos da relação entre alguns cultivares e as espécies Meloidogyne incognita e M. javanica. Viçosa: UFV,  42 p. (Dissertação de Mestrado).        [ Links ]

Fuzzato, M. G.; Cia, E.; Chiavegato, E. J.  1994. Estabilidade da produção de genótipos de algodoeiro em face da ocorrência de doenças e nematóides. Bragantia, 53(1):  47-52.         [ Links ]

Mendes, B. V.; Cardoso, E. J. B. N.; Fernandes, E. R.  1978. Histopatologia de raízes de Feijoeiro parasitadas por Meloidogyne javanica. Sociedade Brasileira de Nematologia, 3: 109-115.        [ Links ]

Menten, J. O. M.; Lordello, L. G. E.; Tulmann Neto, A.; Ando, A. 1980. Nematóides Associados ao Feijoeiro (Phaseolus vulgaris L.) no estado de São Paulo: Informações preliminares. Sociedade Brasileira de Nematologia, 4: 205-212.        [ Links ]

Moura, R. M.; Regis, E. M. O. 1987. Reações de cultivares de feijoeiro comum (Phaseolus vulgaris) em relação ao parasitismo de Meloidogyne javanica e M. incognita (Nematoda: Heteroderidade). Nematologia Brasileira. 11: 215-225.        [ Links ]

Nass, L. L.; Valois, A. C. C.; Melo, I. S.; Valadares-Inglis, M. C. 2001. Recursos e melhoramento: plantas. Rondonópolis: Fundação MT, 1183 p.        [ Links ]

Pedrosa, E. M. R.; Moura, R. M.; Silva, E. G.  2000. Respostas de genótipos de Phaseolus vulgaris à meloidoginose e alguns mecanismos envolvidos na reação. Fitopatologia Brasileira,  25(2): 190-196.         [ Links ]

Ribeiro, C. A. G.; Ferraz, S. 1983. Resistência varietal do Feijoeiro (Phaseolus vulgaris L.) a Meloidogyne javanica. Sociedade Brasileira de Nematologia, 7: 261-270.        [ Links ]

Simão, G.; Homechin, M.; Santiago, D. C.; Silva, R. T. V.; Ribeiro, E. R. 2005. Comportamento de duas cultivares de feijoeiro em relação a Meloidogyne javanica. Ciência Rural, 35 (2): 266-270.         [ Links ]

Vieira, C. 1988. Doenças e pragas do feijoeiro. 1ª edição, Viçosa: Editora UFV. 231.        [ Links ]

Zaumeyer, W.J.; Thomas, H.R. 1957. A monografic study of bean diseases and methods for their control. Washington: Technical Bulletin, 865: 255.        [ Links ]

Zauza, E.A.V.; Alfenas, A.C.; Mafia, R.G.  2007. Esterilização, preparo de meios de cultura e fatores associados ao cultivo de fitopatógenos. In: Alfenas, A.C. & R.G. Mafia (Ed). Métodos em fitopatologia. Editora UFV, Viçosa MG. 22-51.        [ Links ]

Fecha de Recepción: 15 Octubre 2010. Fecha de Aceptación: 19 Abril 2011.

Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons