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Latin american journal of aquatic research

On-line version ISSN 0718-560X

Lat. Am. J. Aquat. Res. vol.43 no.5 Valparaíso Nov. 2015

http://dx.doi.org/10.3856/vol43-issue5-fulltext-9 

Research Article

 

Biometrie sexual and ontogenetic dimorphism on the marine catfish Genidens genidens (Siluriformes, Ariidae) in a tropical estuary

Biometría sexual y dimorfismo ontogenético en el bagre marino Genidens genidens (Siluriformes, Ariidae), en un estuario tropical

 

Larissa G. Paiva1, Luana Prestrelo1,2, Kiani M. Sant' Anna1 & Marcelo Vianna1

1Laboratório de Biologia e Tecnologia Pesqueira, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho 373 Bl. A, 21941-902 Rio de Janeiro, RJ, Brazil
2
Fundação Instituto de Pesca do Estado do Rio de Janeiro, Escritório Regional Norte Fluminense II Avenida Rui Barbosa 1725, salas 57/58, Alto dos Cajueiros, Macaé, RJ, Brazil

Corresponding author: Larissa G. Paiva (larissagpbio@hotmail.com)
Corresponding editor: Guido Plaza


ABSTRACT. This paper aims to study the ontogenetic sexual dimorphism of Genidens genidens in Guanabara Bay, southeastern coast of Brazil. Altogether 378 specimens were anayzed (233 females and 145 males) with total length ranging from 13.3 to 43.5 cm. Specimens were measured for 12 body measurements, sex was identified and maturity stages were recorded and classified. Pearson's linear correlation reveled a significant positive correlation between total length and all other body measures, except for base adipose fin, mouth depth and eye depth for immature females. Analyses nested PERMANOVA desing showed significant differences between maturity stages for each sex, between sexes considering or not maturity stages, indicating a variation in morphometric characteristics driven by sexual dimorphism. Differences among all maturity stages were also found, indicating an ontogenetic morphological difference. But immature individuals didn't differ between sexes indicating that differentiation patterns starts with sexual development. The most important measures differing males and females were related to head characteristics, which appears to be key parameters to evaluate sexual differences. Due to male incubation of fertilized eggs and juvenile individuals <59 mm in their oral cavity, head measures are proposed to be sex dimorphism not related to reproduction, but with post reproductive fase due to ecological and biological needs.

Keywords: Genidens genidens, catfish, Teleostei, morphometry, biometry, dimorphism, tropical estuary.


RESUMEN. Se analizó el dimorfismo sexual y ontogenético del bagre marino Genidens genidens en la Baía de Guanabara, costa sureste de Brasil. Se capturó un total de 378 ejemplares (233 hembras y 145 machos) con longitud total entre 13,3 y 43,5 cm. Se realizaron 12 medidas morfométricas, además de identificar el sexo y los estadios de madurez de los individuos. La correlación lineal de Pearson reveló correlación positiva significativa entre la longitud total y otras medidas corporales excepto la base de la aleta adiposa, altura de la boca y altura de los ojos en hembras inmaduras. Los análisis de PERMANOVA anidados mostraron diferencias significativas entre los estados de madurez de cada sexo, entre los sexos, considerando o no, los estados de madurez, lo que indica una variación en las características morfométricas reguladas por el dimorfismo sexual. También se encontraron diferencias entre los estadios de madurez, lo que indica una diferencia ontogenética para el sexo y estadios de madurez. Los individuos inmaduros no difirieron entre los sexos, indicando que los patrones de diferenciación se inician sólo con el desarrollo sexual. Las medidas más importantes que difieren entre machos y hembras se relacionaron con las características de la cabeza que, al parecer, serían los principales parámetros para evaluar las diferencias sexuales. Debido a que los machos realizan la incubación en su cavidade oral de los huevos fertilizados y de individuos juveniles <59 mm, se sugiere que las medidas de la cabeza corresponden a un dimorfismo sexual no relacionado con la reproducción, pero vinculado a una fase post-reproductiva debido a sus necesidades ecológicas y biológicas.

Palabras clave: Genidens genidens, bagre, Teleostei, morfometría, biometría, dimorfismo, estuario tropical.


 

INTRODUCTION

It is well known that morphology is directly related to species life history and habitat use. Thus fish morphometric analysis represents an important tool to determine their systematic, growth variation, population parameters and environmental relationships (Kováč et al., 1999; Pathak et al., 2013; Sampaio et al., 2013; Souza et al., 2014). Morphometry cover several study fields such as: ecomorphology, relating species morphology with environment characteristics and evaluating the role of environmental preasures on shaping species diet, feeding behavior, ecological strategies, niche partitioning, habitat use and trophic structure (Peres-Neto, 1999; Haas, 2010; Manimegalai et al., 2010; Palmeira & Monteiro-Neto, 2010; Souza et al., 2014) population ecology and metapopulations studies, investigating differences in body shape among populations spatially isolated (Gunawickrama, 2007; Mwanja, 2011; Santos & Quilang, 2012; Sampaio et al., 2013; Souza et al., 2014) and taxonomy, to differ and describe species and taxonomic groups based on internal and external features, which can result in misidentification if the individual is of diferent life stage than the ones used for classification (Marceniuk, 2005a, 2005b). However, intraspecific characteristics are often forgotten in studies investigating species morphological diversity, mainly in taxonomic studies. When this occurs, males and females of the same species may be identified as different species, therefore information about morphological sexual variation is important to avoid species misleading identification (Rapp Py-Daniel & Cox-Fernandes, 2005; Marceniuk, 2007).

Sexual dimorphism can be an important evolutionay adaptation mechanism, conditioning sexual selection and diminishing intraspecific competition by encreasing nich partioning (Hedrick & Temeles, 1989; Herler et al., 2010), being an important study fill whithin morphometric research. An organism must function properly in all life stages and function and form are strictly related (Galis et al., 1994). Ontogenetic changes can determinate the success of an individual due to the importance of morphological features, environment adaptation and reproductive selection. Thus clarify how morphological changes develop throughout individuals growth is important to establish the relationship between morphology and behaviour, elucidating possible ontogenetic nich shifts and the evolutionary plasticity of an organism (Galis et al., 1994).

Marine catfish of the genus Genidens (Siluriformes, Ariidae) are endemic of South Atlantic coasts and are commercially important estuarine fishes in Brazil (Mendoza-Carranza & Vieira, 2009; Silva-Junior et al., 2013). Due to Ariidae benthic habits and broad diet, they have good potential for biomonitoring studies (Azevedo et al., 2012). Furthermore studies with Genidens genidens (Curvier, 1829) in Guanabara Bay and Santos-São Vicente estuary suggested that they should be used as estuarine sentinels, due to their tolerance to eutrophication and others anthropogenic changes (Azevedo et al., 2012; Silva-Junior et al., 2013). In Guanabara Bay, a Brazilian tropical estuary, G. genidens is one of the most abundant species, occupying shallow waters with low salinity and low water transparency (Rodrigues et al., 2007; Silva-Junior et al., 2013). Guanabara Bay has great economic and social importance due to fishing and navigation activities, industrial surrounding areas and ecological relevance due its importance for many marine and freshwater lifecycle (Vasconcelos et al., 2010). Despite their ecological importance G. genidens's morphological sexual and ontogenetic variations are little known. This paper is the first study aiming to evaluate G. genidens's morphological changes between sexes and through ontogenetic development. It will provide more detailed biometric information on the species which could assist further studies on its biology and ecology.

MATERIALS AND METHODS

Guanabara Bay is located in Rio de Janeiro State, southeastern Brazil (22°40'S, 43°02'W) (Fig. 1). It has an area of 384 km2, 7.6 m of mean depth and has an average water residence time of 20 ± 5 days. It is an estuarine environment with minimum salinity of 25 and maximum of 34.5, low hydrodynamics, small grain size bottom and great influence of freshwater runoff and domestic sewage resolting in a major eutrophicated system (Kjerfve et al., 1997; Quaresma et al., 2000).

 

Figure 1. Study location highlighting Guanabara Bay in Rio de
Janeiro state, southeastern coast, Brazil.

 

Specimens of G. genidens (voucher MNRJ 42040) were collected, twice a month, from August 2010 to September 2011. Samples were collected covering the three most important local fisheries (gill net, bottom trawl and stationary pound net) and all Guanabara Bay habitat zones. All specimens were cooled on ice and then measured for 11 body measures (by convention, always on the left side) using a ichtyometer and a electronic caliper rul with precision of 0.01 mm, without being fixed, according to metric measures proposed by Marceniuk (2005a) and Souza & Barrella (2009) (Fig. 2) (Table 1). Measures were standardized as a percentage of total length (TL), excluding total length's effect on body measures. The determination of sex and sexual maturity stages were made by gonads macroscopic observation through an adaptation of Vazzoler (1996): A = immature, B = in maturation process, C+D = mature.

 

Figure 2. Body measures used to characterize Genidens genidens's biometrics. TL: total
length, AF: base adipose fin, CF: base caudal fin, BL: barbeus length, HL: head length,
CL: upper caudal fin lobe length, ID: interorbital distance, BD: maximum body depth,
ED: eye depth, BW: mouth width, MD: mouth depth. This image is a modification of
Figueiredo & Menezes (1978).

 

Table 1. Description of body measures used to characterize Genidens genidens's biometrics.

 

The degree of association between G. genidens size and body proportions, within sex and maturity stages were tested with Pearson's linear correlation analyses using Statistica 8 software, with the significance level of 0.05.

A multivariate nested PERMANOVA design was used to evaluate differences in maturity stages within each sex [maturity (sex)] (ontogenetic differences) and between sexes within maturity stages [sex (maturity)] (sexual dimorphism). A pair-wise post-hoc test was performed to further investigate differences between groups. This test uses an ANOVA experimental design on the basis of any distance measure, using Monte Carlo permutation method (Anderson, 2005) and provides which factor was most important for data differences. A pair-wise post-hoc test was performed to analyze differences among male and female maturity stages. SIMPER analysis was performed to evaluate witch metric measurements were most important for determining group dissimilarity. These analyses were performed using Primer 6 + PERMANOVA software (Clarke & Gorley, 2006).

RESULTS

A total of 378 specimens were captured, 233 females (immature (A) = 9, in maturation process (B) = 68, mature (C+D) = 156) and 145 males (immature (A) = 27, in maturation process (B) = 53, mature (C+D) = 65). Total length ranged from 13.3 to 43.5 cm (Fig. 3).

 

Figure 3. Frequency distribution of total length, of
Genidens genidens,
for female and male, in Guanabara
Bay, Rio de Janeiro, Brazil.

 

Morphometric features and Pearson's linear correlations are presented in Table 2. Body measures were significant correlated with total length (TL), except for base adipose fin (AF), mouth depth (MD) and eye depth (ED) of immature females (Table 2). Morphometric measures showed significant differences for maturity stages within sex, sex within maturity stages and between sexes, but not for maturity stages alone (Table 3a), indicating a possible alteration in morphometric characteristics driven by sexual dimorphism along with maturation process. The post-hoc test showed a significant difference between sexes for all maturity stages except A, indicating that imamture individuals did not have morphological differences and the differentiation only starts at the beginning of sexual development (Table 3b). SIMPER analysis indicated maximum body depth (BD), upper caudal fin lobe length (CL), head length (HL), barbeus length (BL), interorbital distance (ID) and mouth depth (MD) as the six most important metric measurements descrimitating females from males and the group A from B and C+D.

 

Table 2. Morphometric characteristics, of Genidens genidens, and Pearson's linear
correlation (r), for the associations between total lenght and body proportions, within
sex and maturity stages. Range: minimum and maximum values; ͞x = mean values
(± standard deviation). *P < 0.05. **P < 0.001.

 

Table 3. a) Nested PERMANOVA results,
df: degrees of freedom, F: test value,
P
(MC): Monte Carlo asymptotic P-values.
Significance level 95% (P < 0.05), b)
Post-hoc
pair-wise test between male
and female for each maturity stage.

 

DISCUSSION

The present study evaluated morphological onthogenetic changes in Genidens genidens and found a well defined sexual dimophism reveled through changes in head measures. Those differences are related to the maturity process responsible for differing male from female individuls due to their reproductive role. Sexual dimorphism may be an evolutionary adaptative mechanism favouring sexual selection, acting on males when females for choosing partners for mating and in mate competition, enhancing selection towards certain male traits (Hedrick & Temeles, 1989). When compered with other vertebrates, teleosts have a wide range of sexual dimorphism described, including color, size, shape and feeding mechanisms (Kitano et al., 2007; Herler et al., 2010; Mcgee & Wainwright, 2013). For G. genidens sexual dimorphism has been observed in pelvic fins, that are higher in females compared to males (Barbieri et al., 1992), but it had never been evaluated to ontogenetic variations along with sexual changes until this study.

Ontogenetic differences have been described by most studies based on morphology but they are often related to feeding habits and habitat use (Zimmerman et al., 2009; Lima et al., 2012) and not to diffenreces between sex. We observed differences between male and female in head measures (BD, CL, HL, BL, ID, MD), which we suggest to be the key parameters to evaluate sexual dimorphism in this species. Head measures where bigger in male than female probably due to their different roles on the reproductive process, since marine catfish have parental care where male incubates fertilized eggs and larvae in their mouth (Velasco et al., 2006; Silva-Junior et al., 2013). We know that G. genidens, beyond fertilized eggs, the incubators males may present in their oropharyngeal cavity larvae up to 59 mm (Chaves, 1994). Changes in head measures also have been observed in fish species, related with oral incubation behavior. Herler et al. (2010), investigated the sexual dimorphism in populations of the cichlid genus Tropheus, oral incubators fishes, in Lake Tanganyika, in Africa, and found significant differences in mean shape between sexes among the seven populations analyzed related to oral landmarks with larger head length and ventral area (buccal region) in females (the oral breeders). Barnett & Bellwood (2005) investigated the sexual dimorphism in seven fish species with incubation behaviour of eggs and larvae in the oral cavity, in Lizard Island, and observed that males had bigger oral volumes than females in five of them.

For some fishes, clear morphometric differences between sexes appear only in certain gonadal stages in specific body measurements, as observed in this study. Manimegalai et al. (2010) studied the ciclhid Etroplus maculates in India and observed significant correlation between body length growth and morphometric measure and suggested that body measures increases as a function of total growth. Kitano et al. (2007) studied Gasterosteus aculeatus (Gasterosteidae) and also observed sexual dimorphism only after the fish became reproductively mature. Lima et al. (2012) studied the development and allometric growth patterns of the Ariidae catfishes Cathorops spixii and C. agassizii and observed that after hatching, mouth-breeded free embryos of both species grow isometric in all body regions, suggesting that they already bear most of characteristics of adult fish. They suggested that the quick growth of morphometric features related to sensorial organs before hatching, reflect the developmental priorities during the earliest stages when important sensorial organs are being developed for juvenile survival strategies. Our study suggests that the priorities in development of specific body features of G. genidens returns when it reaches maturity and the proportional development of body measures and individual growth can occur differently between male and female especially due different reproduction roles between sexes. Thus when sexual maturity starts males concentrate their growth in head features increasing breeding capacity and reproduction success while female maintain their normal growth.

Our study showed the importance of considering ontogenetic changes related to sex in G. genidens since changes in morphological measures observed around the mouth region is a feature related to male's mouth breeding behavior during reproductive period. Thus the observations made in the present study related to changes in morphometric characteristics in male G. genidens are important to assist future studies about species biology and morphology. Moreover our study also highlight that the same ontogenetic changes may occur with other Ariidae species due to the family's similar reproductive behavior (Figueiredo & Menezes, 1978), thus further studies should be develop aiming to elucidate this pattern which can be essential for a better understanding of the biology, behaviour and life history of those species as well as to determine differences between populations (Kitano et al., 2007).

Marceniuk (2005a, 2005b) discribed Ariidae species of Brazilian coast based on morphological characteristics but didn't observed sexual variations to differenciate species groups. Those studies considered morphological characteristics, neurocranium patterns and vomero-palatine tooth patches. Despite the sex and ontogenetic variation observed in our study for G. genidens, Marceniuk's works are important identification guides for Ariidae family since they are based on other body characteristics besides morphometric measures.

Morphometric estimates differ in degree of precision due to variation in fixation and storage methods and sometimes body structures are injured, making accurate measurements difficult. Nevertheless, due to the importance of these structures for reproduction, we assume that this bias is minimal. The parameters obtained here are realistic and our analysis showed an important role of head measurements in sex dependent ontogenetic differentiation driven by species behavior. We suggest that these measurements are secondary sexual characters not directly related to reproduction.

ACKNOWLEDGEMENTS

To all directly or indirectly involved in this study, especially to colleagues of the Laboratory of Fishery Biology and Technology for their assistance in collecting and biometrics, the fishermen who supplied fish for the study, the Long Term Ecological Program -CNPq (PELD program, process 403809/2012-6) and FAPERJ (Thematic Programme, process E-26/110. 114/2013).

 

REFERENCES

Anderson, M.J. 2005. Permanova: a Fortran computer program for permutational multivariate analysis of variance. Department of Statistics, University of Auckland, Auckland, 24 pp.         [ Links ]

Azevedo, J.S., J.E.S. Sarkis, M.A. Hortellani & R.J. Ladle. 2012. Are catfish (Ariidae) effective bioindicators for Pb, Cd, Hg, Cu and Zn? Water Air Soil Pollut., 223(7): 3911-3922.         [ Links ]

Barbieri, L.R., R.P. Santos & J.V. Andreata. 1992. Reproductive biology of the marine catfish, Genidens genidens (Siluriformes, Ariidae), in the Jacarepaguá Lagoon system, Rio de Janeiro, Brazil. Environ. Biol. Fish., 35(1): 23-35.         [ Links ]

Barnett, A. & D.R. Bellwood. 2005. Sexual dimorphism in the buccal cavity of paternal mouthbrooding cardinalfishes (Pisces: Apogonidae). Mar. Biol., 148: 205-212.         [ Links ]

Chaves, P.T.C. 1994. Eggs and larvae mouthbreeding in Genidens genidens (Valenciennes) (Siluriformes, Ariidae) from Guaratuba Bay, Paraná, Brazil. Rev. Bras. Zool., 11(4): 641-648.         [ Links ]

Clarke, K.R. & R.N. Gorley. 2006. Primer v6: user manual/tutorial. Plymouth, Plymouth Marine Laboratory, 190 pp.         [ Links ]

Figueiredo, J.L. & N.A. Menezes. 1978. Manual de peixes marinhos do sudeste do Brasil. Teleostei (1). MZUSP, São Paulo, 113 pp.         [ Links ]

Galis, F., A. Terlouw & J.W.M. Osse. 1994. The relation between morphology and behaviour during ontogenetic and evolutionary changes. J. Fish Biol., 45(A): 13-26.         [ Links ]

Gunawickrama, K.B.S. 2007. Morphological heterogeneity in some estuarine populations of the catfish Arius jella (Ariidae) in Sri Lanka. Ceylon J. Sci. Biol. Sci., 36(2): 101-108.         [ Links ]

Haas, T.C., M.J. Blum & D.C. Heins. 2010. Morphological response of a stream fish to water impoundment. Biol. Lett., 6: 803-806.         [ Links ]

Hedrick, A.V. & E.J. Temeles. 1989. The evolution of sexual dimorphism in animals: hypotheses and tests. Trends Ecol. Evol., 4(5): 136-138.         [ Links ]

Herler, J., M. Kerschbaumer, P. Mitteroecker, L. Postl & C. Sturmbauer. 2010. Sexual dimorphism and population divergence in the lake Tanganyika cichlid fish genus Tropheus. Front. Zool., 7: 4.         [ Links ]

Kitano, J., S. Mori & C.L. Peichel. 2007. Sexual dimorphism in the external morphology of the threespine stickleback (Gasterosteus aculeatus). Copeia, 2007(2): 336-349.         [ Links ]

Kjerfve, B., C.H.A. Ribeiro, G.T. Dias, A.M. Filippo & V.S. Quaresma. 1997. Oceanographic characteristics of an impacted coastal bay: Baía de Guanabara, Rio de Janeiro, Brazil. Cont. Shelf Res., 17(13): 1609-1643.         [ Links ]

Kováč, V., G.K. Copp & M.P. Francis. 1999. Morphometry of the stone loach, Barbatula barbatula: do mensural characters reflect the species life history thresholds? Environ. Biol. Fish., 56(1-2):105-115.         [ Links ]

Lima, A.R.A., M. Barletta, D.V. Dantas, F.E. Possato, J.A.A. Ramos & M.F. Costa. 2012. Early development and allometric shifts during the ontogeny of a marine catfish (Cathorops spixii-Ariidae). J. Appl. Ichthyol., 28(2012): 217-225.         [ Links ]

Manimegalai, M., S. Karthikeyeni, S. Vasanth, S. Arul-Ganesh, T. Siva-Vijayakumar & P. Subramanian. 2010. Morphometric analysis - A tool to identify the differents in a fish species E. maculatus. Int. J. Environ. Sci., 1(4): 52-56.         [ Links ]

Marceniuk, A.P & N.A. Menezes. 2007. Systematics of the family Ariidae (Ostariophysi, Siluriformes), with a redefinition of the genera. Zootaxa, 1416: 3-126.         [ Links ]

Marceniuk, A.P. 2005a. Redescrição de Genidens barbus (Lacépède, 1803) e Genidens machadoi (Miranda-Ribeiro, 1918), Bagres marinhos (Siluriformes, Ariidae) do Atlântico Sul Occidental. Pap. Avulsos Zool., 45(11): 111-125.         [ Links ]

Marceniuk, A.P. 2005b. Key for identification of the sea catfishes species (Siluriformes, Ariidae) of the Brazilian coast. Bol. Inst. Pesca, São Paulo, 31(2): 89-101.         [ Links ]

Mcgee, M.D. & P.C. Wainwright. 2013. Sexual dimorphism in the feeding mechanism of threespine stickleback. J. Exp. Biol., 216: 835-840.         [ Links ]

Mendoza-Carranza, M. & J.P. Vieira. 2009. Ontogenetic niche feeding partitioning in juvenile of white sea catfish Genidens barbus in estuarine environments, southern Brazil. J. Mar. Biol. Assoc. U.K., 89(4): 839-848.         [ Links ]

Mwanja, M.T., V. Muwanika, S. Nyakaana, C. Masembe, D. Mbabazi, R. Justus & W.W. Mwanja. 2001. Population morphological variation of the Nile perch (Lates niloticus, L. 1758), of East African lakes and their associated waters. Afr. J. Environ. Sci. Technol., 5(11): 941-949.         [ Links ]

Palmeira, L.P. & C. Monteiro-Neto. 2010. Ecomorphology and food habits of teleost fishes Trachinotus carolinus (Teleostei: Carangidae) and Menticirrhus littoralis (Teleostei: Sciaenidae), inhabiting the surf zone off Niterói, Rio de Janeiro, Brazil. Braz. J. Oceanogr., 58(4): 1-9.         [ Links ]

Pathak, N.B., A.N. Parikh & P.C. Mankodi. 2013. Morphometric analysis of fish population from two different ponds of vadodara city, Gujarat, India. J. Anim. Veter. Fish. Sci., 1(6): 6-9.         [ Links ]

Peres-Neto, P.R. 1999. Alguns métodos e estudos em ecomorfologia de peixes de riachos. Oecol. Bras., 6: 209-236.         [ Links ]

Quaresma, V.S., G.T.M. Dias & J.A. Baptista. 2000. Characterization of side-scan sonar and high resolution seismic (3.5-7.0 kHZ) reflection patterns along the southern margin of the Guanabara Bay-RJ. Rev. Bras. Geofis., 18(2): 201-214.         [ Links ]

Rapp Py-DanieL, L.R. & C. Cox-Fernandes. 2005. Sexual dimorfism in amazonian siluriformes and gymnotiformes (Ostariophysi). Acta Amaz., 35(1): 97-110.         [ Links ]

Rodrigues, C., H.P. Lavrado A.P.C. Falcão & S.H.G. Silva. 2007. Distribuição da ictiofauna capturada em arrastos de fundo na Baía de Guanabara, Rio de Janeiro, Brasil. Arq. Mus. Nac., 65: 199-210.         [ Links ]

Sampaio, A.L.A., J.P.A. Pagotto & E. Goulart. 2013. Relationships between morphology, diet and spatial distribution: testing the effects of intra and interspecific morphological variations on the patterns of resource use in two Neotropical cichlids. Neotrop. Ichthyol., 11(2): 351-360.         [ Links ]

Santos, B.S. & J.P. Quilang. 2012. Geometric morphometric analysis of Arius manillensis and Arius dispar (Siluriformes: Ariidae) populations in Laguna de Bay. Philipp. J. Sci., 141(1): 1-11.         [ Links ]

Silva-Junior, D.R., D.M.T. Carvalho & M. Vianna. 2013. The catfish Genidens genidens (Cuvier, 1829) as a potential sentinel species in Brazilian estuarine waters. J. Appl. Ichthyol., 9(6): 1297-1303.         [ Links ]

Silva-Junior, L.C., A.C. Andrade & M. Vianna. 2011. Length-weight relationships for elasmobranchs from southeastern Brazil. J. Appl. Ichthyol., 27(6): 1408-1410.         [ Links ]

Souza, C.E. & W. Barrella. 2009. Atributos ecomorfológicos de peixes do Sul do Estado de São Paulo. Rev. Electron. Biol., 2(1): 1-34.         [ Links ]

Souza, M.A., D.C. Fagundes, C.G. Leal & P.S. Pompeu. 2014. Ecomorphology of Astyanax species in estrems with diferente substrates. Zoologia, 31(1): 42-50.         [ Links ]

Vazzoler, A.E.A.M. 1996. Biologia da reprodução de peixes teleósteos: teoria e prática. EDUEM, Maringá, 196 pp.         [ Links ]

Vasconcelos, R.P., P. Reis-Santos, A. Maia, V. Fonseca, S. França, N. Wouters, N.M.J. Costa & H.N. Cabral. 2010. Nursery use patterns of commercially important marine fish species in estuarine systems along the portuguese coast. Estuar. Coast. Shelf Sci., 86(4): 613-624.         [ Links ]

Velasco, G., E.G. Reis & J.P. Vieira. 2006. Calculating growth parameters of Genidens barbus (Siluriformes, Ariidae) using length composition and age data. J. Appl. Ichthyol., 23(1): 64-69.         [ Links ]

Zimmerman, M.S., S.N. Schmidt, C.C. Krueger, M.J. Van der Zanden & R.L. Eshenroder. 2009. Ontogenetic niche shifts and resource partitioning of lake trout morphotypes. Can. J. Fish Aquat. Sci., 66: 1007-1018.         [ Links ]

 


Received: 28 November 2014;
Accepted: 18 August 2015

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