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Journal of soil science and plant nutrition

versión On-line ISSN 0718-9516

J. Soil Sci. Plant Nutr. vol.15 no.3 Temuco set. 2015  Epub 30-Abr-2015

http://dx.doi.org/10.4067/S0718-95162015005000024 

 

Interaction effects of nitric oxideand salicylic acid in alleviating salt stress of Gossypium hirsutum L.

 

Y.J. Dong1,2,3 , Z.L. Wang 2, J.W. Zhang 2, S. Liu1, Z.L. He3 , M.R. He 2*

1College of Resources and Environment, Shandong Agricultural University, Tai'an 271018, Shandong P.R.China.

2College of Agronomic Sciences, Shandong Agricultural University, Tai'an 271018, Shandong Province, P.R.China.

*Corresponding author: mrhe@sdau.edu.cn

3University of Florida, Institute of Food and Agricultural Sciences, Indian River Research and Education Center, 2199 S. Rock Road, Fort Pierce, FL 34945, United States.


 

Abstract

The effects of sodium nitroprusside (SNP, a nitric oxide donor), salicylic acid (SA), and their combination on some physiological parameters of cotton (Gossypium hirsutum L.) seedlings grown under saline conditions were studied. 100 mM NaCl resulted in a decrease in , and induced an increase in the level of electrolyte leakage, malondialdehyde (MDA) content, O2.- , H2O2 and proline contents.0.1 mM SNP decreased Na+ and Cl- accumulation as well as the lipid peroxidation level and O2.- accumulation in leaves, which contributed to alleviate oxidative stress in cotton leaves. 0.1 mM SA alleviated the oxidative stress by increasing K+ and Mg2+ absorption and osmotic regulators accumulation, and decreasing the H2O2 content. However, the protective action of NO and SA (especially 0.05 mM SNP and 0.05 mM SA) against salt-induced oxidative damage was more efficient than the separate effects of SA and SNP.We observed that the accumulation of osmotic regulators was apparently accelerated by 0.05 mM SNP and 0.05 mM SA. As well, it was observed that they had synergistic effects in antioxidant system and ion absorption. Thus, the combination of SNP and SA can be a new indicator of a protective mechanism to lower lipid peroxidation, improve ion absorption, activate the metabolic of osmotic-regulated substances and facilitate membrane transport to detoxify reactive oxygen species (ROS).

Keywords: Antioxidant enzymes, cotton, nitric oxide, NaCl, oxidative stress, salicylic acid


 

1. Introduction

Salinity is one of the important factors affecting growth and metabolic responses of crops throughout the world (Deinlein et al., 2014). Salt-affected plants deteriorate as a result of ROS production such as the superoxide radical (O2·-), hydrogen peroxide (H2O2), hydroxyl radical (·OH) and singlet oxygen (1O2) that can damage mitochondria and chloroplasts by disrupting cellular structures (Tariq et al., 2011). Excess of ROS triggers phytotoxic reactions such as lipid peroxidation, enzymes inactivation and protein degradation. These changes lead to osmotic and ion-specific effects as well as to imbalances in plant nutrition, which may range from deficiencies in several nutrients (Qadir et al., 2006). To mitigate and repair the damage, plant cells detoxify ROS by the synchronous action of various antioxidants constituted of non-enzymatic and enzymatic components. Superoxide dismutase (SOD), peroxidase (POX), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) are the enzymatic antioxidants, while water soluble components (sugars and proteins) and the lipid-soluble components (carotenoids and α -tocopherol) are the non- enzymatic antioxidants (Sheetal et al., 2012). Hence, the alleviation of the oxidative damage and increased resistance to salinity and other environmental stresses are often correlated with an efficientantioxidative system and an accumulation of water-soluble component.

Nitric oxide (NO) is a small, highly diffusible gas and an ubiquitous bioactive molecule. Its chemical properties make NO a versatile signal molecule that functions through the interactions with cellular targets via either redox or additive chemistry (Lamattina et al., 2003). Undoubtedly, there are many studies show that NO itself is the plant signaling molecule, and today it is known to play a crucial role in regulating many key physiological processes in different plants, such as in rice, cucumber, mung bean and so on (Panda et al., 2011). Various mechanisms contribute to NO-induced increase of salt tolerance. First, NO might be involved in increasing the antioxidant content and antioxidant enzyme activity to scavenge ROS. Second, NO might increase risk element accumulation in root cell walls and decrease the risk element accumulation in the soluble cell fraction to maintain the cellular redox homeostasis and regulation of the leaves (Panda et al., 2011; Manai et al. 2014). Finally, NO could function as a signaling molecule in the cascade of events leading to changes in gene expression under risk element stress (Xiong et al., 2009). However, an access amounts of NO may interact with O2·-to form peroxynitrite (ONOO-), which has been reported to damage lipids, proteins and nucleic acids.Nevertheless, O2.- and H2O2 are more toxic than NO and ONOO-. This type of dual roles of NO as a potent oxidant or effective antioxidant generally depends on the status of the environments (Panda et al., 2011) and also contents.

Salicylic acid (SA) is another signaling molecule or chemical messenger and its role in the defense mechanism is well-established under stress conditions (Gunes et al., 2005). A large number of studies have reported the effects of exogenous SA on alleviating environment stress, including salt (Misra et al., 2009), heavy metal (Sanjib et al., 2007), water and chilling, by the stimulation of growth. SA has been reported to ameliorate the adverse effects of salinity by improving the functioning of ion uptake and transport, membrane permeability, and antioxidative enzymes (Gunes et al., 2005). Furthermore, SA is also considered to be a signaling molecule that plays a key role in growth, development, and defense responses in plants under stress conditions. Although a few authors have reported the effects of exogenous NO and SA alleviating salt toxicity in different plants by various mechanisms, information is still scant regarding the influences of NO and SA on salt tolerance improvement in cotton seedlings.

Cotton (Gossypium hirsutum L.) has been considered as an important crop for fibre production in several countries. This species can grow well in saline areas, indicating tolerance to salt stresses (Silva et al., 2010; Wang et al. 2014); however, it is sensitive to salt in the seedling stage (Liu et al., 2013). Therefore in the present study, we suppose that NO and SA may ameliorate toxic effects of excess Na on cotton. Furthermore, it is also of a great interest to know whether exogenous NO and SA could act as a regulator of antioxidant intervention strategy in preventing leaf oxidative stress in response to NaCl stress to improve the oxidation resistance capacity, and to get a better understanding of how plants adjust to an adverse environment.

2. Material and Methods

2.1. Plant materials and experimental design

Before sowing, healthy seeds of cotton (Gossypium hirsutum L.) were surface-sterilized with 1% sodium hypochlorite for 10 min, and then vigorously rinsed with distilled water. The seeds were sown in plastic pots filled with matrix. The pots were arranged in a growth chamber (28/20 oC; day/night, light intensity 150 µmol/m2/s, 14-hour photoperiod, 60 % relative humidity) to cultivate. After germination, 7-day-old seedlings were cultivated in a hydroponics system in the growth chamber. 100 mM NaCl (Sigma, USA) without or with SNP (0.05 or 0.1 mM, Sigma, USA) or SA (0.05 or 0.1 mM, Sigma, USA) were added to Hoagland solution during the third week of seedling growth (We determine the concentrations used for SNP, SA and NaCl in this study according to the experiment of ourselves (Liuet al., 2013) and the experiment of other person (Dong et al.,2014).

SNP was used as a donor of NO. The Hoagland solution was daily renewed.

2.2. Chemical treatment

NaCl, SNP and SA treatments were as follows: (1) control, CK; (2) 100 mM NaCl (T1); (3) 100 mM NaCl+0.1 mM SNP (NO, T2); (4) 100 mM NaCl+0.1 mM SA (SA, T3); (5) 100 mM NaCl+0.05 mM SNP+0.05 mM SA (1/2(NO+SA, T4); (6) 100 mM NaCl+0.1 mM SNP+0.1 mM SA (NO+SA, T5). The plants were sampled at 15 days after the treatment. The nutrient solution pH was adjusted to 6.8. Each of the glassware included 5 seedlings and represented one replicate, and there were three replicates per treatment. The treatment solution was changed everyday to maintain constant NaCl concentration.

2.3. Determination of NO levels

NO levels were determined according to the method described by Chen et al., (2010). Fresh samples were cut into approximately 5-mm segments and immediately incubated with 100 U CAT and 100 U SOD for 5 min to remove endogenous ROS before addition of oxyhemoglobin (5 mM). After 3 min of incubation, NO was quantified by spectrophotometric measurement of the conversion of oxyhemoglobin to methemoglobin.

2.4. Determination of SPAD reading

The portable chlorophyll meter (SPAD-502, Minolta Camera Co., Japan) can estimate total chlorophyll amounts in the leaves of a variety of species with a high degree of accuracy, which is a non-destructive method. Measurements were taken at 4 locations on each leaf; two on each side of the midrib on all fully expanded leaves and then averaged (Khan et al., 2003).

2.5. Determination of ion concentration

For determination of ion contents, 100 mg of ground dry material of the leaf, stem and roots were digested by adding 4 mL HClO4 at 200 ¡æ until a colorless liquid was formed. After digestion, each sample was brought up to a 25 mL final volume with distilled-deionized water. The Na+ and K+ contents were determined with a flame photometer. The Ca2+ and Mg2+ contents were measured by flame atomic absorbance spectrometry (SHIMADZU AA-6300, Kyoto, Japan). The Cl- content was measured in accordance with Tsukada (2005).

2.6. Determination of electrolyte leakage and lipid peroxidation

Electrolyte leakage was used to assess the cell membrane permeability as described by Tariq et al., (2011). The leaves were rinsed three times with distilled water. Plant material (5-mm leaf discs) was transferred to each test vial containing 10 mL of double-distilled water. The vials were sealed and then placed on a rotary shaker for 24 h. The electric conductivity (EC1) of the content of the vial was determined, using an electrical conductivity meter (Metzer Optical Instruments, India). The vials were again sealed and autoclaved at 120 °C for 20 min. The contents of the vials were cooled at room temperature, followed by recording the electric conductivity (EC2) again. Membrane damage was calculated using the equation: MD (%) = (EC1/EC2) x100.

Malondialdehyde (MDA) content uses the thiobarbituric acid reaction method (Heath & Packer, 1968). Fresh samples (0.2 g) were ground in 3 mL of trichloroacetic acid (0.1 %, w/v). The homogenate was centrifuged at 10,000xg for 10 min and 1 ml of the supernatant fraction was mixed with 4 mL of 0.5 % thiobarbituric acid (TBA) in 20 % TCA. The mixture was heated at 95 °C for 30 min, chilled on ice, and then centrifuged at 10,000xg for 5 min. The absorbance of the supernatant was measured at 532 nm. The value for non-specific absorption at 600 nm was subtracted. The amount of MDA was calculated using the extinction coefficient of 155 mM-1 cm-1 and expressed as nmol g-1 FW.

2.7. Determination of endogenous ROS production

The second fully expanded leaves of the plants were sampled for enzymatic analysis. Samples were homogenized in 0.05 M phosphate buffer (pH 7.8) by grinding with a mortar and pestle under ice-cold condition. The homogenate was filtered through four layers of muslin cloth and centrifuged at 12,000 xg for 10 min at 4 °C, and the supernatants were used for measurements of O2.-generation rate according to Sui et al. (2007). Fresh samples (1 g) were homogenized in 2 ml ice-cold acetone. Titanium reagent (2 % TiCl2 in conc. HCl) was added to one milliliter of extract supernatant to give a Ti (IV) concentration of 2 %. The Ti-H2O2 complex, together with unreacted Ti, was then precipitated by adding 0.2 ml 17 M ammonia solution for each 1 ml of extract. The precipitate was washed five times with ice acetone by resuspension, drained, and dissolved in 1 M H2SO4 (3 ml). The absorbance of the solution was measured at 410 nm against blanks prepared similarly, however without plant tissue (Sui et al., 2007).

2.8. Determination of proline content

Proline was determined as described by Simaei et al., (2011). Fresh leaf tissues (0.5 g) from each treatment were homogenized in 10 mL of 3 % w / v sulphosalicylic acid and the homogenate was filtrated. The resulting solution was treated with 2.5 % ninhidrine solution and glacial acetic acid. In test tubes, the reaction mixtures were kept in a water bath at 100 °C for 60 min to develop the colors. Soon after removal from the water bath, the test tubes were cooled in ice bath and toluene was added to separate chromophores. Optical density was read at 520 nm using UV-VIS spectrophotometer (Shimadzu UV-2210).

2.9. Determination of soluble sugar content

Plants from each treatment/control were washed thoroughly with distilled water, cut into small pieces, weighed, placed separately in glass vials containing 10 mL of 80 % (v / v) ethanol, and heated at 60 oC for 30 min. The extract was then filtered and diluted with 80 % (v / v) ethanol up to 20 ml. The concentrations of soluble sugar were determined in this extract as described by Giannakoula et al. (2008).

2.10. Enzyme extraction and enzyme assays

For determination antioxidant enzymes, 0.5 g powder of freeze-dried leaves were homogenized with 50 mM Na2HPO4-NaH2PO4 buffer (pH 7.8) containing 0.2 mM EDTA and 2 % insoluble polyvinylpyrrolidone in a chilled pestle and mortar. The slurry was centrifuged at 12,000 xg for 20 min and the supernatant was used for enzyme activities assay. The experiment was carried out at 4 °C.

Total SOD activity was assayed by the photochemical method (Rao and Sresty 2000). The 3 mL reaction mixture contained 50 mM phosphate buffer (pH 7.8), 13 mM methionine, 75 µM nitroblue tetrazolium, 2 mM riboflavin, 10 mM EDTA and 0.1 mL enzyme extract. One unit of the enzyme activity was defined as the amount of enzyme required to result in a 50 % inhibition of the rate of nitro blue tetrazolium (NBT) reduction measured at 560 nm. SOD activity was expressed as U g-1 FW min-1. Guaiacol peroxidase (POD) activity was estimated after Nickel and Cunningham (1969) method. Activity was measured by the increase in absorbance at 470 nm due to guaiacol oxidation. The activity was expressed as U g-1 FW min-1. CAT activity was determined by following the consumption of H2O2 at 240 nm according to the method of Cakmak and Marschner (1992). CAT activity was expressed as nmol H2O2 mg-1 FW min-1.

2.11. Statistical analysis

Each pot was treated as one replicate, and all the treatments were repeated three times. Excel 2003 software to process data and draw the table, the DPS software (DPS 7.05) for statistical analysis, and the least significant difference (LSD) was calculated to compare the differences between means in each treatment.

3. Results

3.1. NO Production

NO concentration in the leaves and roots increased dramatically after 5 days of NaCl treatment relative to the control (Figure 1). T1 increased leaves NO content by 37.86 % compared to CK. T2 and T4 also increased leaves NO content by 98.92 and 109.49 %, respectively. Root NO concentration was similar to that of leaves of NaCl exposure. The NO content was significantly increased in roots under NaCl stress, but this increase was also effectively enhanced by application of exogenous SNP or/and SA, especially T4 treatment.

Figure 1. NO content in leaves and roots of cotton seedlings exposed to NaCl and as affected by SNP and SA. Values are the mean of three replicates

Note: Values are the mean of three replicates. The values followed by the different letter show statistically significant differences at P<0.05. CK-control, T1-100 mM NaCl, T2-0.1 mM SNP, T3-0.1 mM SA, T4-0.05 mM SNP + 0.05 mM SA, T5-0.1 mM SNP + 0.1 mM SA.

To prove that the SNP-released NO, rather than other substances associated with SNP decomposition (such as cyanide and Fe), was responsible for the SNP-induced alleviation effect on NaCl toxicity, NaCl-treated cotton seedlings were treated with 100 μM K4Fe(CN)6 and 100 μM SNP + 100 μM cPTIO separately. And the plant fresh weight and dry weight were measured. The results indicated that treatment with neither 100 μM SNP + 100 μM cPTIO nor 100 μM K4Fe(CN)6 had an ameliorating effect on NaCl-induced inhibition of cotton seedlings growth (Figure 2). This evidence proves the hypothesis that SNP-release NO, rather than other substances of SNP decomposition, accounts for the alleviation effect on NaCl toxicity.

Figure 2. Effects of SNP, cPTIO and K4Fe(CN)6 on FW and DW in cotton seedlings subjected to NaCl stress. FW, fresh weight (g plant-1); DW, dry weight (g plant-1).

Note: Values are the mean of three replicates. The values followed by the different letter show statistically significant differences at P<0.05. CK-control, T1-100 mM NaCl, T2-0.1 mM SNP, T3-0.1 mM SA, T4-0.05 mM SNP + 0.05 mM SA, T5-0.1 mM SNP + 0.1 mM SA.

3.2. SPAD reading

As shown in Figure 3A, the trend of SPAD reading change was determined during ten days. The SPAD reading was significantly decreased in T1 after 6 days, SNP or/and SA treatments can alleviate the symptoms. T1 treatment sharply decreased the SPAD reading by 32.9 % with CK on the tenth day. T2 elevated the decreased the reading by 21.34 % compared with T1 treatment, whereas T4 substantially enhanced concentration of chlorophyll on the tenth day, which was enhanced 36.67 % compared with T1 treatment.

Figure 3. Effects of SNP and SA on SPAD reading (A) in cotton seedlings subjected to NaCl stress in ten days; Effects of SNP and SA for NaCl on proline, soluble protein and soluble sugar contents (B) in cotton seedlings subjected to NaCl stress. proline (µmol g-1, FW); soluble protein (mg g-1, FW); soluble sugar (mg g-1, FW). Values are the mean of three replicates.

Notes: Values are the means of three replicates. The values followed by the different letter show statistically significant differences at P<0.05. CK-control, T1-100 mM NaCl, T2-0.1 mM SNP, T3-0.1 mM SA, T4-0.05 mM SNP + 0.05 mM SA, T5-0.1 mM SNP + 0.1 mM SA.

3.3. Na , K, Ca, Mg, and Cl concentrations

As shown in Table 1, in NaCl-treated plants, Na accumulated more in shoots than in roots. In the presence of SNP or/and SA, Na content decreased in roots and shoots, as compared to T1 treatment. Cl concentration was similar to that of Na in NaCl exposure. However, in contrast to Na accumulation, salinity significantly decreased K, Ca and Mg content of cotton plants. Conversely, there was a slight rise in salt-stressed plants supplemented with SNP or/and SA than NaCl treated only (Table 1).

Table 1. Effects of SNP and SA on Na (%), K (%), Ca (g kg-1), Mg (g kg-1), and Cl contents (mg g-1) in cotton seedlings subjected to NaCl stress

Note: Values are the mean of three replicates. The values followed by the different letter show statistically significant differences at P<0.05. CK-control, T1-100 mM NaCl, T2-0.1 mM SNP, T3-0.1 mM SA, T4-0.05 mM SNP + 0.05 mM SA, T5-0.1 mM SNP + 0.1 mM SA

3.4. Membrane damages and electrolyte leakage

For the control plants, the level of MDA was low, but under T1 treatment, cotton seedlings showed higher concentrations of MDA. Both SNP and SA used in this study caused a decrease in the MDA contents (Table 2). Simultaneously, T4 (the combination of SNP and SA) is remaining comparable to CK.

Table 2. Effects of SNP and SA on MDA content, electrolyte leakage and antioxidant enzymes in cotton seedlings subjected to NaCl stress. MDA content (µmol g-1 FW); Electrolyte leakage (%); O2.- generation rat (µmol g-1 h-1); H2O2 (µmol g-1 FW); CAT, catalase, (µmol H2O2 mg-1 min-1 FW); POD, peroxidase, (U mg-1 min-1 FW); SOD, superoxide dismutase (units g-1 FW).

Notes: Values are the mean of three replicates. The values followed by the different letter show statistically significant differences at P<0.05. CK-control, T1-100 mM NaCl, T2-0.1 mM SNP, T3-0.1 mM SA, T4-0.05 mM SNP + 0.05 mM SA, T5-0.1 mM SNP + 0.1 mM SA.

NaCl treated plants exhibited a significant increase in electrolyte leakage. Application of SNP or/and SA decreased the level of electrolyte leakage in salt stressed plants (Table 2). T1 resulted in a 44.92 % increase in electrolyte leakage as compared with CK whereas the electrolyte leakage was decreased by 24.87 % and 13.85 % in T4 and T5 treated plants as compared with T1.

3.5. Endogenous ROS production and osmotic regulators accumulation

The results show that excess NaCl led to an enhancement in H2O2 content in leaves, up to 0.95 times (p < 0.05) higher than those of CK plants, indicating an evident oxidative stress occurred in the NaCl-stressed cotton leaves (Table 2). Application of SA or SNP, especially T4 treatment, significantly reduced H2O2 accumulation in NaCl-stressed plants. Similar to the moderating effect of H2O2, the SNP or SA could significantly decrease the O2·- generation rate that induced by salt.

Soluble protein in cotton leaves increased with 100 mM NaCl levels (Figure 3B). And it increased in SNP or SA addition more sharply. T4 caused the most accumulation which was especially significant of CK. In addition, total proline showed a similar trend. T1 increased the content of soluble sugar measured in the leaves of salt-treated cotton plants. When compared to CK, salt stress increased soluble sugar of cotton plants by 2.15-fold. When SNP or SA was applied to the plants, soluble sugar in the leaves showed a little decreased. Moreover, T4 almost nullified the effect of stress caused by NaCl and improved the soluble sugar significantly.

3.6. Antioxidant enzymes

Since NaCl treatment caused oxidative stress in cotton plants, we examined the behavior of the antioxidant enzymes next. SOD activity was inhibited by 17.85 % in leaves of cotton plants treated with T1 as compared with CK. When SNP and SA were added to nutrient solution containing NaCl, SOD activity increased by 17.67 % and 27.94 % respectively in leaves. The POD activity in leaves of T1-treated plants decreased by 15.92 % as compared with CK. When NaCl-stressed plants were treated with SA or SNP, POD activity significantly increased in leaves. Whereas treated with T4, in NaCl-treated plants, the POD activity was increased by 17.43 % compared with NaCl-treated alone. The CAT activity in leaves of T1-treated plants was decreased by 38.94 % as compared with CK. When SA and SNP were added to nutrient solution containing NaCl, the CAT activity of leaves and roots increased. In NaCl-treated plants, SNP or SA, especially T4 improved the enzyme activity in comparison to NaCl -treated plants.

4. Discussion

Nitric oxide (NO) as a ubiquitous signal molecule participates in major physiological processes in plant growth and development, and in response to biotic and abiotic stresses (Wilson, 2008). A notable increase of NO content was detected in the cotton seedlings exposed to 100 mM NaCl, whereas there was more increase in that of the SNP treatment under NaCl-stress (Figure 1). NaCl-induced inhibition of fresh and dry weight was significantly reversed when cotton seedlings were treated with SNP (Figure 2), compared with the treatment of the cPTIO and K4Fe(CN)6. These evidences proved the hypothesis that SNP-released NO, rather than other substances of SNP decomposition, accounts for the alleviation effect on NaCl toxicity.

In this work, we analyzed the possible role of exogenous SNP and SA in the modulation of the antioxidant defense system against NaCl stress in cotton seedlings. Evidences have been demonstrated that the interaction between NO andSA had synergistic effects in decreasing of the damages induced by all kinds of abiotic stress (Simaei et al., 2011; Gil¡,vanova et al., 2012). In the present study, we were able to increase the cotton antioxidant capacity and its components under salinity by applying NO and SA. Measurement of SPAD reading under salinity conditions explained that NaCl has the negative effects of chlorophyll contents. Treated with NO or / and SA, especially the combination of SNP and SA, decreased these damages induced by salt (Figure 3A). The decrease in SPAD reading in salt-affected plants might be attributed to the possible oxidation of chlorophyll and other chloroplast pigments coupled with instability of the pigment protein complex under salt stress (Tariq et al., 2003). Exogenous SNP and SA lead to the promotion of protective reactions to the photosynthetic pigments and induce a pre-adaptive response to salt stress (Simaei et al., 2011), which confirms our results.

In addition, the correlations between and among variousnon-enzyme indices (e.g., ions content, leaf electrolyte leakage, MDA content, H2O2 content, O2.- generation rate, proline contents, and soluble sugar) and enzyme indices (e.g., CAT, POD, and SOD) in cotton seedlings subjected to salt stress were analyzed. Results showed that significant cor-relations existed between these non-enzyme indices and enzyme parameters. The contents of Na and Cl increased in leaves, stems and roots of the cotton under NaCl treatment, while an opposite trend for K, Ca and Mg content was obtained (Table 1). Na and Cl, entering into the cytoplasm through the cell membrane, lead to their accumulation in the cytoplasm, decreasing the availability of some of the essential nutrients as well (Deinlein, 2014). On the other hand, the excess of Na in the nutrient media led to the accumulation of H2O2 in cotton leaves and SNP/SA led to eliminating the accumulation (Table 2). High concentration of Na caused membrane disorganization. As the second messenger, Ca in cytosolic was increased, might induce further physiological response including the expression of osmotic responsive genes for salt adaptation in plants. This facilitation could be attributed to the induction of antioxidant responses and elevated Ca uptake that protects the plants from the oxidative damage by SNP and SA (El-tayeb et al., 2005). In addition, alteration of mineral uptake from NO and SA applications may be one mechanism for the alleviation of salt stress.

Also, excessive accumulation of Na in the cytosol has an adverse effect on maintaining a stable electrical conductivity of solution. Electrolyte leakage indicates cell membrane injury when plants are subject to salinity stress. The leaves electrolyte leakage was significantly affected by the salt stress treatments, but not the NO/SA concentrations and their interactions (Table 2). Maintenance of the integrity of cellular membranes under salt stress is considered an integral part of the mechanism of salinity tolerance (Tariq et al., 2003). Studies suggested the SA facilitated maintenance of membrane functions through induction of antioxidant mechanisms and elevated ion uptake, thereby protecting the plants against the oxidative damage (El-tayeb et al., 2005). While, NO effectively reduce the level of ROS generated during stress, and thus, limit oxidative damage in plant cells (Arasimowicz et al., 2007). According to the cited authors, the application of NO and SA, which facilitated maintenance of membrane functions under salt stress, could be an indicator of the build-up of a protective mechanism to reduce cytoplasm fluid leakage induced by salt stress.

Application of NO and/or SA reduced NaCl-induced increase in the content of MDA (Table 2). It was suggested that superoxide anion (O2·-), H2O2, radicals of hydroxyl (OH·), lipid alcoxyl (LO·), and lipid peroxyl (LOO·), which are produced during salinity, can be the major factors responsible for lipid peroxidation. The level of MDA, a product of lipid peroxidation, has been considered an indicator of oxidative damage (Li et al., 2008). Decreased lipid peroxidation against oxidative stress, may assist in overcoming severe stresses and providing a rapid recovery. Li (2008) also indicated that salt stress could cause damages to the integrity of the cellular membrane such as lipids that was sensitive to oxidative stress. SA was reported to inhibit production of hydroxyl radical and to decrease the content of MDA (Gunes et al., 2005). Proline is able to scavenge hydroxyl radical and stabilize the structure and function of macromolecules and structures, such as DNA, protein, and membranes (Misra et al., 2009). It can be clearly seen that NaCl induced proline accumulation (Figure 3B). It has been reported that SA, through the induction of ABA-mediated proline production, can decrease the deleterious effects of salt stress and water deficit in wheat seedlings (Sakhabutdinova et al., 2003). Because MDA and proline are often considered a compatible solute involved in osmotic adjustment under stress, reduction in the level of MDA and increase in the level of proline in (NO+SA)-treated plants indicate the stress amelioration role of NO and SA that might be responsible for maintenance of plant growth under stress.

Salinity has been shown to reduce water uptake by roots because of lowered osmotic potentials of the substrate, and to cause changes in metabolic activities leading to the reduction in plant growth (Jaleel et al. 2007). The overall reduction in the growth of cotton seedlings might be ascribed to the ill effect of salt stress on the various physiological processes such as photosynthesis, ion homeostasis, accumulation of compatible solutes, and activities of antioxidant enzymes. The content of soluble protein is significantly increased in the leaves at the NaCl-treated (Figure 3B). With respect to the salinized plants, soluble protein content increased significantly in leaves with T4 treatment thanother treatments. SA treatment induced more accumulation of soluble sugar of the stressed plants (Khodary et al., 2009).Soluble sugar can be involved in, or related to, ROS-producing metabolic pathways. It might either directly detoxify ROS in chloroplasts and vacuoles or indirectly stimulate the classic antioxidative defense systems Khodary et al., 2009). In this regard, it is assumed that, 1/2 (SNP+SA) may stimulate hydrolysis of proteins, providing a pool of compatible osmolyte, which is important in osmotic adjustment in the presence of sodium.

Furthermore, electron transport processes, such as photosynthesis and respiration, generate basal levels of H2O2, which is increased in response to stress (Simaei et al., 2011). Under salt stress, plants are overloaded with ROS, which inhibit several plant processes and cause damage to plants in different ways. Thus, to maintain metabolic functions under stress, scavenging of ROS is essential. ROS scavenging depends on detoxification mechanisms provided by antioxidant enzymes. The increase in the activities of CAT and SOD in NaCl-treated plants has been documented by various workers investigating salinity stress in several plant species (Tariq et al., 2011). In this regard, CAT appears to play an essential protective role in scavenging ROS in coordination with SOD. SOD initiates detoxification of singlet oxygen by producing H2O2, which is eliminated by its conversion to H2O in subsequent reactions. It was suggested that H2O2 was key regulators of plant responses to a range of endogenous signals, which are involved in developmental processes, such as seed germination, and root development (Khan et al., 2003). SNP and SA-induced CAT and SOD activities increase indicate that they can play a critical role in modulating the cell redox balance, thereby protecting plants against oxidative damage. In our study, the CAT and SOD activities were higher in T5 (0.1mM NO+0.1mM SA) were higher than T4 (0.05 mM SNP + 0.05 mM SA), which maybe associated with promoting the absorption of K, but the reason need further research. Contents of endogenous H2O2 and O2·– were measured to determine the internal ROS status induced in cotton exposure to salinity treatments. Contents of H2O2 and O2·– were higher in plants treated with NaCl, indicating an increase in ROS level of the cells in salt-treated plants. Among the antioxidant enzymes, CAT and SOD are well known ROS scavengers. When the ROS contents exceed their threshold level under stress, they are scavenged by these enzymes. SNP and SA treatment increased CAT and SOD activities to scavenge the elevated level of ROS in this study.

5. Conclusion

In summary, SNP combined with SA (0.05 mM SNP+0.05 mM SA) could play a better role to reduce oxidative stress unlike them alone, improve Ca2+/Mg2+ and decrease Na+ absorption, enhance the activity of antioxidant enzymes, trigger the accumulation of endogenous osmotic regulators for the facilitation of the membrane transport and ROS detoxifying. The data also showed only the optimum concentration of SNP and SA could act directly on oxidative stress to promote salt-tolerance and plant growth.

Abbreviations: CAT-Catalase; DW-Dry weight; FW-Fresh weight; H2O2-Hydrogen peroxide; MDA-Malondialdehyde; NaCl-Sodium chloride; NO-Nitric oxide; O2•−-Superoxide anion; POD-Peroxidases; ROS-Reactive oxygen species; SNP-Sodium nitroprusside; SA -Salicylic acid; SOD-Superoxide dismutase

Acknowledgements

The authors thank English lecturer Xiujuan Wang (College of Foreign Languages, Shandong Agricultural University) for their critical reading and revision of the manuscript. Special acknowledgements are given to the editors and reviewers. This work was financially supported by the Chinese National Basic Research Program (2015CB150404), indigenous innovation project of Shandong province (2014 ZZCX07401) and a Project of Shandong Agricultural University saline and alkaline land (75030).

References

Arasimowicz, M., Floryszak-Wieczorek, J. 2007. Nitric oxide as a bioactive signalling molecule in plant stress responses. Plant Sci. 172, 876-887.         [ Links ]

Chen, F., Wang, F., Sun, H., Cai, Y., Mao, W., Zhang, G., Vincze, E., Wu, F. 2010. Genotype-Dependent Effect of Exogenous Nitric Oxide on Cd-induced Changes in Antioxidative Metabolism, Ultrastructure, and Photosynthetic Performance in Barley Seedlings (Hordeum vulgare). J. Plant Growth Regul. 29, 394-408.         [ Links ]

Cakmak, I., Marschner, H. 1992. Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves. Plant Physiol. 98, 1222-1227.         [ Links ]

Deinlein, U., Stephan, A.B., Horie, T., Luo, W., Xu, G., Schroeder, J.I. 2014. Plant salt-tolerance mechanisms. Trends Plant Sci 19(6):371-379.         [ Links ]

Dong, Y. , Jinc, S., Liu, S., Xu, L., Kong, J. 2014. Effects of exogenous nitric oxide on growth of cotton seedlings under NaCl stress. Journal of Soil Science and Plant Nutrition. 14(1), 1-13.         [ Links ]

El-tayeb, M. 2005. Response of barley grains to the interactive effect of salinity and salicylic acid. Plant Growth Regul. 45, 215-224.         [ Links ]

Giannakoula, A., Moustakas, M., Mylona, P., Ioannis, P., Traianos, Y. 2008. Aluminium tolerance in maize is correlated with increased levels of mineral nutrients, carbohydrates and proline and decreased levels of lipid peroxidation and Al accumulation. J. Plant Physiol. 165, 385-396.         [ Links ]

Gunes, A., Inal, A., Alpaslan, M., Cicek, N., Esra, G., Figen, E., Tutku, G. 2005. Effects of exogenously applied salicylic acid on the induction of multiple stress tolerance and mineral nutrition in maize (Zea mays L.). Archives of Agronomy and Soil Science. 51, 687-695.         [ Links ]

Gil¡,vanova, I.R., Enikeev, A.R., Stepanov, S., Rakhmankulova, Z.F. 2012. Involvement of salicylic acid and nitric oxide in protective reactions of wheat under the influence of heavy metals. Appl. Biochem. Micro. 48(1), 90-94.         [ Links ]

Heath, R.L., Packer, L. 1968. Photoperoxidation in isolated chloroplasts. I: kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics. 125,189-198.         [ Links ]

Jaleel, C.A., Gopi, R., Panneerselvam, R. 2007. Alterations in lipid peroxidation, electrolyte leakage, and proline metabolism in Catharanthus roseus under treatment with triadimefon, a systemic fungicide. Comptes Rendus Biologies. 330(12), 905-912.         [ Links ]

Khan, W., Prithiviraj, B., Smith, D.L. 2003. Photosynthetic responses of corn and soybean to foliar application of salicylates. Journal of Plant Physiology. 160, 485-492.         [ Links ]

Khodary S.E.A . 2009. Effect of salicylic acid on the growth, photosynthesis and carbohydrate metabolism in salt-stressed maize plants. International Journal of Agriculture and Biology. 6, 5-8.         [ Links ]

Lamattina, L., Garcia-Mata, C., Graziano, M., Pagnussat, G. 2003. Nitric oxide: the versatility of an extensive signal molecule. Annu. Rev. Plant Biol. 54, 109-136.         [ Links ]

Li, Q., Niu, H., Yin, J., Wang, M., Shao, H., Deng, D., Chen, X., Ren, J., Li, Y. 2008. Protective role of exogenous nitric oxide against oxidative-stress induced by salt stress in barley (Hordeum vulgare). Colloid Surface B. 65, 220-225.         [ Links ]

Liu, S., Dong, Y., Xu, L.,Kong, J., Bai, X. 2013. Roles of exogenous nitric oxide in regulating ionic equilibrium and moderating oxidative stress in cotton seedlings during salt stress. Journal of Soil Science and Plant Nutrition. 13 (4), 929-941.         [ Links ]

Lu, X., Wang, D., Yin, Z., Wang, J., Fan, W., Wang, S., Zhao, X., Zhang, T., Ye, W. 2014. Genomic DNA Methylation Polymorphism Analysis of Cotton Under NaCl and Na2CO3Stress. Scientia Agricultura Sinica. 47(16), 3132-3142.         [ Links ]

Manai, J., Kalai, T., Gouia, H., Corpas, F.J. 2014. Exogenous nitric oxide (NO) ameliorates salinity-induced oxidative stress in tomato (Solanum lycopersicum) plants. Journal of Soil Science and Plant Nutrition. 14 (2), 433-446.         [ Links ]

Misra, N., Saxena, P. 2009. Effect of salicylic acid on proline metabolism in lentil grown under salinity stress. Plant Science. 177, 181-189.         [ Links ]

Nickel, K.S, Cunningham, B.A. 1969. Improved peroxidase assay method using Leuco 2,3,6-trichloro indophenol and application to comparative measurements of peroxidase catalysis. Ann. Clin. Biochem. 27, 292-299.         [ Links ]

Panda, P., Nath, S., Thorny, C.T., Sharma, G.D., Panda, S.K. 2011. Cadmium stress-induced oxidative stress and role of nitric oxide in rice (Oryza sativa L.). Acta Physiol Plant. 3, 1737-1747.         [ Links ]

Qadir, M., Noble, A.D., Schubert, S., Thomas, R.J., Arslan, A. 2006. Sodicity-induced land degradation and its sustainable management: problems and prospectives. Land Degrad. Dev. 17, 661-676.         [ Links ]

Rao, K.V.M., Sresty, T.V.S. 2000. Antioxidative parameters in the seedlings of pigeon pea (Cajanus cajan L. Millspaugh) in response to Zn and Ni stresses. Plant Sci. 157, 113-128.         [ Links ]

Simaei, M., Khavarinejada, R.A., Saadatmanda, S., Bernardb, F., Fahimia, H. 2011. Interactive Effects of Salicylic Acid and Nitric Oxide on Soybean Plants under NaCl Salinity. Russian Journal of Plant Physiology. 5, 783-790.         [ Links ]

Sanjib, K.P., Hemanta, K.P. 2007. Effect of salicylic acid potentiates cadmium-induced oxidative damage in Oryza sativa L. leaves. Acta Physiol. Plant. 29, 567-575.         [ Links ]

Sakhabutdinova, A.R., Fatkhutdinova, D.R., Bezrukova, M.V., Shakirova, F.M. 2003. Salicylic Acid Prevents the Damaging Action of Stress Factor in Wheat Plants. Bulg. J. Plant Physio. SPECIAL ISSUE. 314-319.         [ Links ]

Sui, N., Liu, X.Y., Wang, N., Fang, W., Meng, Q.W. 2007. Response of xanthophylls cycle and chloroplastic antioxidant enzymes to chilling stress in tomato over-expressing glycerol-3-phosphate acyltransferase gene. Photosynthetica.  45, 447-454.         [ Links ]

Silva, E.N., Ferreira-Silva, S.L., Fontenele, A.V., Ribeiro, R.V., Viégas, R.A., Silveira, J.A.G. 2010. Photosynthetic changes and protective mechanisms against oxidative damage subjected to isolated and combined drought and heat stresses in Jatropha curcas plants. J. Plant Physiol. 167, 1157-1164.         [ Links ]

Sheetal, C., Sunita, J., Veena, J. 2012. Salinity induced oxidative stress and antioxidant system in salt-tolerant and salt-sensitive cultivars of rice (Oryza sativa L.). Journal of Plant Biochemistry and Biotechnology. doi: 10.1007/s13562-012-0107-4.         [ Links ]

Tariq, A., Masroor, M., Khan, A., Jaime, A., Teixeira, D.S., Mohd, I., Naeem, M. 2011. Role of Salicylic Acid in Promoting Salt Stress Tolerance and Enhanced Artemisinin Production in Artemisia annua L.J. Plant Growth Regul. 30, 425-435.         [ Links ]

Tsukada, H., Takeda, A., Hasegawa, H., Ueda, S., Iyogi, T., Radioanal, J. 2005. Nucl. Chem. 263, 773.         [ Links ]

Wang ,X., Mohamed, I., Xia, Y., Chen, F. 2014. Effects of water and potassium stresses on potassium utilization efficiency of two cotton genotype. Journal of Soil Science and Plant Nutrition. 14 (4), 833-844.         [ Links ]

Wilson, I.D., Neill, S.J., Hancock, J.T. 2008. Nitric Oxide synthesis and signaling in plants. Plant Cell Environ. 31, 622-631.         [ Links ]

Xiong, J., An, L.Y., Lu, H., Zhu, C. 2009. Exogenous nitric oxide enhances cadmium tolerance of rice by increasing pectin and hemicellulose contents in root cell wall. Planta. 230, 755-765.         [ Links ]

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